Assessment of polycyclic aromatic hydrocarbon biodegradation potential in mangrove sediment from Don Hoi Lot, Samut Songkram Province, Thailand

Abstract

Aims: To assess the biodegradation potential of mixed polycyclic aromatic hydrocarbons (PAHs) in mangrove sediments. Methods and Results: Sediment microcosms were constructed with sediment collected from Don Hoi Lot, Samut Songkram Province, Thailand, by supplementation with a mixture of acenaphthene, phenanthrene and pyrene. At the end of 8 weeks, low molecular weight PAHs, acenaphthene and phenanthrene were completely degraded. PCR-denaturing gradient gel electrophoresis profile suggests that Marinobacter, Enterobacter and Dethiosulfatibacter play important roles in PAH degradation in mangrove sediment. Furthermore, six PAH-degrading bacteria were isolated consisting of novel phenanthrene-degrading Dyella sp. and Luteibacter sp., phenanthrenedegrading Burkholderia sp., acenaphthene-degrading Alcaligenes sp. and pyrenedegrading Ochrobactrum sp. Moreover, dioxygenase genes could be detected both in sediment microcosms as well as in all of the isolated strains. Conclusions: These results demonstrated that indigenous bacteria in the mangrove sediment had the ability to degrade phenanthrene in the presence of mixture PAHs with high efficacy. Significance and Impact of the Study: Culture and nonculture methods were combined to assess PAH biodegradation in mangrove sediment. Two novel phenanthrene-degrading bacteria were isolated. Three genera of bacteria that play important roles in PAH degradation were indicated by nonculture approach. Moreover, dioxygenase genes could be detected. This information is useful for further bioremediation of PAH-contaminated mangrove sediments.

Bioaugmentation and biostimulation as strategies for the bioremediation of a burned woodland soil contaminated by toxic hydrocarbons: A comparative study

“…In particular, among bacteria, Dyella kyunghensis P15, a gram negative soil bacterial strain, was able to transform Congo Red. In this respect, it is worth noting that the potential for hydrocarbon degradation had already been ascertained in the related species D. japonica (Muangchinda et al, 2013). On the other hand, further bacterial isolates, namely Ochrobactrum sp.…”

Treatment of High-Concentration Wastewater from an Oil and Gas Field via a Paired Sequencing Batch and Ceramic Membrane Reactor

“…Furthermore, sequence JX910137.1 exhibited a 94% similarity with Luteibacter sp. characterized by Muangchinda et al [28], and HM187346.1 had a 99% agreement with the HDB-SIST494 16S RNA gene characterized by Lin. [29] in deep oilfield water.…”

“…Furthermore, sequence JX910137.1 exhibited a 94% similarity with Luteibacter sp. characterized by Muangchinda et al [28], and…”

Degradation of Phenolic Compounds in Palm Oil Mill Effluent by Silica‐Immobilized Bacteria in Internal Loop Airlift Bioreactors

“…DNA was extracted from the wastewater samples and the silica‐immobilized bacteria according to the method described by Ausubel et al . The expression of the largest subunit of the phenol hydroxylase (LmPH) gene was quantified using the U1 + U2 + U3F/UR primers as described by Basile and Erijman , while 16S rRNA gene expression was quantified using the 968F/1401R primers as described by Muangchinda et al . The LmPH gene has been used as a molecular marker to assess functional and genetic diversities of phenol‐degrading bacteria in the environment.…”