Assessment of some bread wheat (Triticum aestivum L.) genotypes for drought tolerance using SSR and ISSR markers

Abstract

As a result of the rapid increase in the world population, the need for wheat, which is one of the main nutrition in the human diet, is also rapidly increases. However, due to yield losses caused by abiotic stress factors such as drought, wheat production is not sufficient. Therefore, genetic characterization studies performed on wheat genotypes in terms of drought tolerance are important. In this study, genetic characterization of wheat genotypes regardingdrought tolerance was carried out by using molecular markers associated with drought-tolerance genes. For this purpose, 14 polymorphic markers were used to be able to distinguish between the control groups. Genetic characterization of 27 bread wheat genotypes by using eight ISSR markers revealed a polymorphism rate of 75.8%, and the mean PIC was calculated as 0.55. Based on the results of the genetic characterization performed with six SSR markers, the mean PIC value was 0.77, the mean He was 0.79, and the mean allele number was 6.7. In this study, the characterization of drought-tolerant and sensitive genotypes was carried out, and the potentials of genotypes for breeding studies were revealed. This study also indicates that used SSRs and ISSRs markers are useful in marker-assisted breeding about drought tolerance.

Molecular and Morphological Characterization of Wheat Genotypes under Drought Condition in Nigerian Sudan Savannah

“…They reported the reliability and significance of SSR markers in genetic characterization of drought tolerance in bread wheat. Other studies were conducted using 27 bread wheat genotypes to evaluate their response to drought stress through six SSR and eight ISSR markers which they reported significant genetic diversity among the genotypes which could be utilized for marker assisted selection and other breeding programs (Ates-Sonmezoglu et al, 2022). Yadav et al (2018) reported significant variation using 15 ISSR markers for six drought tolerance and six drought sensitive wheat varieties in which 14 markers gave reproductive bands.…”

Zinc accumulation in wheat, and how it's affected by genetics and sulphate of zinc.

“…[53] and Özlem et al [54] reported that ISSR markers provided greater recurrence, polymorphism, and the ability to distinguish across bread wheat cultivars.…”

Molecular Genetic Diversity of Local and Exotic Durum Wheat Genotypes and Their Combining Ability for Agronomic Traits under Water Deficit and Well-Watered Conditions

“…Fifteen SSR markers were utilized in this study. The applied SSR markers were identified from earlier studies based on their consistent association with drought tolerance in wheat [31][32][33]. The sequences of applied primers are detailed in Table 1.…”