Assessment of the biopotency of follitropin alfa and lutropin alfa combined in one injection: a comparative trial in Sprague-Dawley rats

Abstract

Mixing follitropin alfa RFF and lutropin alfa did not alter the bioactivity of either FSH or LH.

Advances in Recombinant Gonadotropin Production for Use in Bovine Superovulation

“…This could be accomplished by combining pure, recombinant preparations of each protein in a fixed and controllable ratio. Alper et al. (2008) reported that combining FSH and LH into one dose and treating rats did not affect either of the proteins bioactivity.…”

Administration of follitropin alfa and lutropin alfa combined in a single injection: a feasibility assessment

“…The authors found no evidence of pharmacokinetic or pharmacodynamic interactions between follitropin alfa and lutropin alfa. More recently, it has been demonstrated that r-hLH and r-hFSH can be mixed together in the same syringe without compromising the bioactivity of the gonadotrophins [ 14 ], and administered successfully to patients [ 15 ]. Purified urinary gonadotrophin products can also be reconstituted and mixed together in a single syringe without affecting the FSH or LH bioactivity [ 16 ].…”

An activity-calibrated chemical standardization approach for quality evaluation of Salvia miltiorrhiza Bge.

“…8 Our group had established several biological assay methods for the quality control of TCMs, such as purgative activity assays, cardiac activity assays, antiviral activity assays, and antiplatelet aggregation assays. [8][9][10][11][12][13][14][15][16][17][18] Despite the potential advantage of being able to show close association with clinical efficacy, biological assays still have some limitations, such as limited operability, high cost, low precision, and animal ethics issues, when live animals are used in some instances. 2,[19][20][21][22] However, those limitations are usually not limitations for chemical analyses.…”