Assessment of the repeatability and border-plate effects of the B158/B60 enzyme-linked-immunosorbent assay for the detection of circulating antigens (Ag-ELISA) of Taenia saginata

Jansen, Famke; Dorny, Pierre; Berkvens, Dirk; Hul, Anke Van; Broeck, Nick Van den; Makay, Caroline; Praet, Nicolas; Gabriël, Sarah

doi:10.1016/j.vetpar.2016.07.027

Cited by 7 publications

(5 citation statements)

References 12 publications

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“…Additionally, the method worked on 2 different donors, not only as an end-point assay of tissue health, but also as a noninvasive tool for tracking tissue development during the long maturation period (approximately 35 days) (33). Importantly, the accuracy of the algorithms in predicting both TER and VEGFratio was close to the measurement uncertainty for both TER (31) and VEGF (30,34). The generalizability of such metrics across cell lines, regardless of donor, adds to the broad utility of this work, as here we show that even with a limited data set, strong predictive ability, regardless of donor and clone, was shown ( Figure 5).…”

Section: Discussionmentioning

confidence: 99%

Deep learning predicts function of live retinal pigment epithelium from quantitative microscopy

Schaub¹,

Hotaling²,

Manescu³

et al. 2020

Journal of Clinical Investigation

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Section: Discussionmentioning

confidence: 99%

Deep learning predicts function of live retinal pigment epithelium from quantitative microscopy

Schaub¹,

Hotaling²,

Manescu³

et al. 2020

Journal of Clinical Investigation

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“… The most promising antigens are members of the 8 kDa diagnostic antigen family [ 44 , 46 , 50 ], used as fusion proteins/peptides, and recombinant proteins derived from the glycoproteins GPT24 and GP50 [ 47 , 49 , 54 ], employed independently or as “antigen cocktail”. Antigen detection CSF, serum • The antigen-capture ELISAs based on the use of HP10 [ 55 – 57 ] or B158/B60 [ 58 – 61 ] monoclonal antibodies, prepared against T. saginata antigens, are the two diagnostic options employed in both detection and follow-up of NCC patients, mainly in patients with several active cysts and extraparenchymal locations, and in epidemiological studies in endemic regions. DNA detection CSF, biopsy • The amplification protocols (polymerase chain reaction, PCR) are the same described for the taeniosis diagnosis (Table 4 ), but applied to CSF as clinical sample.…”

Section: Discussionmentioning

confidence: 99%

Present status of laboratory diagnosis of human taeniosis/cysticercosis in Europe

Morales

Gárate

Blocher

et al. 2017

Eur J Clin Microbiol Infect Dis

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Human cysticercosis (CC) is a parasitic zoonosis caused by the larval stage (cyst) of the Taenia solium. Cysts can establish in the human central nervous system (neurocysticercosis, NCC) and other organs and tissues; they also develop in pigs, the natural intermediate host. Human taeniosis may be caused by T. solium, Taenia saginata and Taenia asiatica tapeworms; these infections are usually asymptomatic, but show a significant relevance as they perpetuate the parasites’ life cycle, and, in the case of T. solium, they are the origin of (N)CC. In European Union (EU) member states and associated countries, the occurrence of autochthonous T. solium cases is debated, and imported cases have significantly increased lately; the status of T. asiatica has been never reported, whereas T. saginata is prevalent and causes an economic impact due to condemned carcasses. Based on their effects on the EU society, the specific diagnosis of these pathologies is relevant for their prevention and control. The aims of this study were to know the diagnostic tests used in European laboratories for human taeniosis/cysticercosis by means of a questionnaire, to determine potential gaps in their detection, and to obtain preliminary data on the number of diagnosed taeniosis/CC cases.Electronic supplementary materialThe online version of this article (doi:10.1007/s10096-017-3029-1) contains supplementary material, which is available to authorized users.

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“…There is thus a world to win for test developers, routine test laboratories, and authorities. An available IA method is used for research purposes only and not suitable for routine analyses [27].…”

Section: Parasitesmentioning

confidence: 99%

Modernization of Control of Pathogenic Micro-Organisms in the Food-Chain Requires a Durable Role for Immunoaffinity-Based Detection Methodology—A Review

Bergwerff

Debast

2021

Foods

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Food microbiology is deluged by a vastly growing plethora of analytical methods. This review endeavors to color the context into which methodology has to fit and underlines the importance of sampling and sample treatment. The context is that the highest risk of food contamination is through the animal and human fecal route with a majority of foodborne infections originating from sources in mass and domestic kitchens at the end of the food-chain. Containment requires easy-to-use, failsafe, single-use tests giving an overall risk score in situ. Conversely, progressive food-safety systems are relying increasingly on early assessment of batches and groups involving risk-based sampling, monitoring environment and herd/flock health status, and (historic) food-chain information. Accordingly, responsible field laboratories prefer specificity, multi-analyte, and high-throughput procedures. Under certain etiological and epidemiological circumstances, indirect antigen immunoaffinity assays outperform the diagnostic sensitivity and diagnostic specificity of e.g., nucleic acid sequence-based assays. The current bulk of testing involves therefore ante- and post-mortem probing of humoral response to several pathogens. In this review, the inclusion of immunoglobulins against additional invasive micro-organisms indicating the level of hygiene and ergo public health risks in tests is advocated. Immunomagnetic separation, immunochromatography, immunosensor, microsphere array, lab-on-a-chip/disc platforms increasingly in combination with nanotechnologies, are discussed. The heuristic development of portable and ambulant microfluidic devices is intriguing and promising. Tant pis, many new platforms seem unattainable as the industry standard. Comparability of results with those of reference methods hinders the implementation of new technologies. Whatever the scientific and technological excellence and incentives, the decision-maker determines this implementation after weighing mainly costs and business risks.

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Assessment of the repeatability and border-plate effects of the B158/B60 enzyme-linked-immunosorbent assay for the detection of circulating antigens (Ag-ELISA) of Taenia saginata

Cited by 7 publications

References 12 publications

Deep learning predicts function of live retinal pigment epithelium from quantitative microscopy

Deep learning predicts function of live retinal pigment epithelium from quantitative microscopy

Present status of laboratory diagnosis of human taeniosis/cysticercosis in Europe

Modernization of Control of Pathogenic Micro-Organisms in the Food-Chain Requires a Durable Role for Immunoaffinity-Based Detection Methodology—A Review

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