Assessment of β-d-glucosidase activity of intact cells of two Oenococcus oeni strains with synthetic and natural substrates

Abstract

The β-d-glucosidase (βG) activity of intact cells of Oenococcus oeni SD-2a and 31MBR was assessed with synthetic and natural substrates, also its partial characterisation was investigated. Results showed that intact cells of both strains showed βG activity, SD-2a performing higher than 31MBR, against either synthetic or natural substrate. The high βG activity of SD-2a intact cells under wine-like temperature, pH, ethanol concentration, and glucose concentration could be of great interest for its utilisation in winemaking. SD-2a proved promising for aroma enrichment in winemaking.

Assessment of β-D-glucosidase activity and bgl gene expression of Oenococcus oeni SD-2a

“…O. oeni SD-2a, one patent strain screened in China, possesses important oenological characteristics, particularly being able to perform MLF effectively under winery conditions. In previous study, βG activity of O. oeni SD-2a was assessed and partially characterized [11,26,29]. In order to enable a better understanding of the capacity of O. oeni SD-2a to aroma formation during MLF.…”

“…Numerous studies had been conducted, providing evidence of the potential βG activity of O. oeni strains for flavor enhancement in wines [7,10,[14][15][16][17][18][19][20][21][22][23][24]. These studies demonstrated that possession of βG activity is widespread and extent of βG activity is strain dependent among O. oeni strains [16,21,25,26], and the enzyme activity is mainly affected by pH, temperature, sugar, and ethanol concentration [15][16][17]19,27,28].…”

“…oeni SD-2a is one patent strain screened from spontaneous MLF wines in Yantai, Shandong Province, China, which with an excellent performance in MLF is widely used in winemaking. In previous study, βG activity of O. oeni SD-2a was localized, assessed with synthetic and natural substrates and partially characterized under different physicochemical conditions and abiotic stresses [11,26,29]. Also, one bgl gene of O. oeni SD-2a was cloned, sequenced and analyzed [24].…”

“…These studies demonstrated that possession of β G activity is widespread and extent of β G activity is strain dependent among O . oeni strains [ 16 , 21 , 25 , 26 ], and the enzyme activity is mainly affected by pH, temperature, sugar, and ethanol concentration [ 15 – 17 , 19 , 27 , 28 ].…”

“…In previous study, β G activity of O . oeni SD-2a was localized, assessed with synthetic and natural substrates and partially characterized under different physicochemical conditions and abiotic stresses [ 11 , 26 , 29 ]. Also, one bgl gene of O .…”