Assignment of Colletotrichum coccodes isolates into vegetative compatibility groups using infrared spectroscopy: a step towards practical application

Salman, Ahmad; Shufan, Elad; Lapidot, Itshak; Tsror, Leah; Moreh, R.; Mordechaǐ, S.; Huleihel, Mahmoud

doi:10.1039/c5an00213c

Cited by 13 publications

(10 citation statements)

References 65 publications

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“…The complementation tests are performed in liquid media in 96-well plates, a method that saves materials, labor, and time, compared to conventional pairings on solid media. Another newly developed technique for determining VCGs dispenses with the nit mutant system altogether, instead relying on spectroscopic analysis to group isolates into VCGs [ 58 ]. Though not yet widely used, these new techniques may transform the way vegetative compatibility testing is performed.…”

Section: Vegetative Compatibilitymentioning

confidence: 99%

Two genomes are better than one: history, genetics, and biotechnological applications of fungal heterokaryons

Strom

Bushley

2016

Fungal Biol Biotechnol

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Heterokaryosis is an integral part of the parasexual cycle used by predominantly asexual fungi to introduce and maintain genetic variation in populations. Research into fungal heterokaryons began in 1912 and continues to the present day. Heterokaryosis may play a role in the ability of fungi to respond to their environment, including the adaptation of arbuscular mycorrhizal fungi to different plant hosts. The parasexual cycle has enabled advances in fungal genetics, including gene mapping and tests of complementation, dominance, and vegetative compatibility in predominantly asexual fungi. Knowledge of vegetative compatibility groups has facilitated population genetic studies and enabled the design of innovative methods of biocontrol. The vegetative incompatibility response has the potential to be used as a model system to study biological aspects of some human diseases, including neurodegenerative diseases and cancer. By combining distinct traits through the formation of artificial heterokaryons, fungal strains with superior properties for antibiotic and enzyme production, fermentation, biocontrol, and bioremediation have been produced. Future biotechnological applications may include site-specific biocontrol or bioremediation and the production of novel pharmaceuticals.

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Section: Vegetative Compatibilitymentioning

confidence: 99%

Two genomes are better than one: history, genetics, and biotechnological applications of fungal heterokaryons

Strom

Bushley

2016

Fungal Biol Biotechnol

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“…Using 13 PCs it was possible to achieve a classification success rate in excess of 96%. Keeping in mind that the infections with these herpes viruses are biologically similar, these results are considered very good [ 40 , 79 – 82 ]. In the second strategy, the classification procedure was performed in two stages.…”

Section: Discussionmentioning

confidence: 99%

Detection of Vero Cells Infected with Herpes Simplex Types 1 and 2 and Varicella Zoster Viruses Using Raman Spectroscopy and Advanced Statistical Methods

et al. 2016

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Of the eight members of the herpes family of viruses, HSV1, HSV2, and varicella zoster are the most common and are mainly involved in cutaneous disorders. These viruses usually are not life-threatening, but in some cases they might cause serious infections to the eyes and the brain that can lead to blindness and possibly death. An effective drug (acyclovir and its derivatives) is available against these viruses. Therefore, early detection and identification of these viral infections is highly important for an effective treatment. Raman spectroscopy, which has been widely used in the past years in medicine and biology, was used as a powerful spectroscopic tool for the detection and identification of these viral infections in cell culture, due to its sensitivity, rapidity and reliability. Our results showed that it was possible to differentiate, with a 97% identification success rate, the uninfected Vero cells that served as a control, from the Vero cells that were infected with HSV-1, HSV-2, and VZV. For that, linear discriminant analysis (LDA) was performed on the Raman spectra after principal component analysis (PCA) with a leave one out (LOO) approach. Raman spectroscopy in tandem with PCA and LDA enable to differentiate among the different herpes viral infections of Vero cells in time span of few minutes with high accuracy rate. Understanding cell molecular changes due to herpes viral infections using Raman spectroscopy may help in early detection and effective treatment.

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“…The hypotheses of these studies are based on the capability of infrared spectroscopy to monitor minor biochemical changes in biological samples associated with the initiation of abnormality due to its high sensitivity [36]. Machine learning algorithms were used to analyze the midinfrared absorption spectra and classify them into their appropriate categories [50,[53][54][55]. Machine learning algorithms were used to analyze the midinfrared absorption spectra and classify them into their appropriate categories [50,[53][54][55].…”

Section: The T-cells Are Divided Into Two Types Helper T-cells (Cd +mentioning

confidence: 99%

“…Thus, we hypothesize that the biochemical changes in leukocytes resulting from the different responses of the human immune system to viral and bacterial infections can be monitored by FTIR spectroscopy for FPOPs under chemotherapy treatment. Machine learning algorithms were used to analyze the midinfrared absorption spectra and classify them into their appropriate categories [50,[53][54][55]. In this study, we used infrared spectroscopy with machine learning algorithms to diagnose the etiology of the sample as bacterial or viral.…”

Section: The T-cells Are Divided Into Two Types Helper T-cells (Cd +mentioning

confidence: 99%

Rapid diagnosis of infection etiology in febrile pediatric oncology patients using infrared spectroscopy of leukocytes

Agbaria

Rosen

Lapidot

et al. 2019

Journal of Biophotonics

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Rapid diagnosis of the etiology of infection is highly important for an effective treatment of the infected patients. Bacterial and viral infections are serious diseases that can cause death in many cases. The human immune system deals with many viral and bacterial infections that cause no symptoms and pass quietly without treatment. However, oncology patients undergoing chemotherapy have a very weak immune system caused by leukopenia, and even minor pathogen infection threatens their lives. For this reason, physicians tend to prescribe immediately several types of antibiotics for febrile pediatric oncology patients (FPOPs). Uncontrolled use of antibiotics is one of the major contributors to the development of resistant bacteria. Therefore, for oncology patients, a rapid and objective diagnosis of the etiology of the infection is extremely critical. Current identification methods are time‐consuming (>24 h). In this study, the potential of midinfrared spectroscopy in tandem with machine learning algorithms is evaluated for rapid and objective diagnosis of the etiology of infections in FPOPs using simple peripheral blood samples. Our results show that infrared spectroscopy enables the diagnosis of the etiology of infection as bacterial or viral within 70 minutes after the collection of the blood sample with 93% sensitivity and 88% specificity.

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Assignment of Colletotrichum coccodes isolates into vegetative compatibility groups using infrared spectroscopy: a step towards practical application

Cited by 13 publications

References 65 publications

Two genomes are better than one: history, genetics, and biotechnological applications of fungal heterokaryons

Two genomes are better than one: history, genetics, and biotechnological applications of fungal heterokaryons

Detection of Vero Cells Infected with Herpes Simplex Types 1 and 2 and Varicella Zoster Viruses Using Raman Spectroscopy and Advanced Statistical Methods

Rapid diagnosis of infection etiology in febrile pediatric oncology patients using infrared spectroscopy of leukocytes

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