Assignment of the human granulocyte colony-stimulating factor receptor gene (CSF3R) to chromosome 1 at region p35–p34.3

Inazawa, Johji; Fukunaga, Rikiro; Seto, Yoshiyuki; Nakagawa, Hitoshi; Misawa, Shinichi; Abe, Tatsuo; Nagata, Shinji

doi:10.1016/0888-7543(91)90202-p

Cited by 46 publications

(23 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Localisation of DNA markers by FISH FISH was performed as described elsewhere (Inazawa et al, 1991). Metaphase chromosomes were prepared by the thymidine synchronisation-BrdU release technique (Takahashi et al, 1990) for delineation of replicated G-bands.…”

Section: Methodsmentioning

confidence: 99%

Deletion mapping on chromosome 1p in well-differentiated gastric cancer

Ezaki

Yanagisawa²,

Ohta³

et al. 1996

Br J Cancer

View full text Add to dashboard Cite

Summary To define the region on the short arm of chromosome 1 that is thought to include one or more tumour-suppressor genes for gastric cancers, we carried out loss of heterozygosity (LOH) studies in 26 gastric adenocarcinomas, using three restriction fragment length polymorphism (RFLP) markers and nine microsatellite markers. All tumours were informative with at least one locus; three revealed replication errors (RERs) at multiple microsatellite loci, and interstitial or telomeric allelic deletions were observed in 12 cases. Deletion mapping of these tumours defined a commonly deleted region between two loci, DlS201 and DlS197, that are 13 cM apart. As two loci within the commonly deleted region, D1S57 (pYNZ2) and D1S62 (pTHI54), were mapped respectively to lp35 and lp34.3 by fluorescence in situ hybridisation, we conclude that a locus likely to contain a tumour-suppressor gene for gastric cancer is located within a 13 cM region encompassing these two chromosomal bands. (Deng et al., 1987;Kihana et al., 1991) and p53 (Tamura et al., 1991;Strickler et al., 1994) genes. Amplification of the erbB-2 gene and mutations of the APC and ras genes are found frequently in well-differentiated adenocarcinomas but not in poorly differentiated adenocarcinomas (Yokota et al., 1988;Kihana et al., 1991;. In contrast, amplification of the K-sam gene and replication errors (RERs) at microsatellite loci have been detected preferentially in poorly differentiated adenocarcinomas (Hattori et al., 1990;Han et al., 1993). These observations imply that the genetic pathways involved in development of these two histopathologically distinguished forms of gastric adenocarcinoma are likely to be different.Recent results of LOH studies have suggested that loci containing tumour-suppressor genes associated with gastric carcinogenesis exist on chromosomal arms lp, lq, 5q, 7q, 12q 17p and 18q (Sano et al., 1991;Uchino et al., 1992; Kuniyasu et al., 1995). As part of a strategy to identify these putative tumour-suppressor genes we began by attempting to define the region on chromosome lp that is commonly deleted in gastric cancers. Here we report results of LOH studies in 26 well-differentiated adenocarcinomas of the stomach. Materials and methodsPreparation of samples and DNA A total of 26 paired samples of tumours and corresponding normal tissues removed from Japanese patients with welldifferentiated adenocarcinomas of the stomach were obtained at the Cancer Institute Hospital, Tokyo. In 18 cases the tissue samples were fixed in formalin, embedded in paraffin and attached individually to glass slides. Genomic DNA was extracted according to methods described elsewhere (Goelz et al., 1985;Yanagisawa et al., 1991). The remaining eight samples were frozen in liquid nitrogen after surgical resection and stored at -80°C until isolation of DNA. Genomic DNA was extracted from the frozen tissues according to methods described elsewhere (Sato et al., 1990).RFLP markers for LOH analysis The three RFLP markers used in this study, D1S77 (pMCT58), DIS57 (...

show abstract

Section: Methodsmentioning

confidence: 99%

Deletion mapping on chromosome 1p in well-differentiated gastric cancer

Ezaki

Yanagisawa²,

Ohta³

et al. 1996

Br J Cancer

View full text Add to dashboard Cite

show abstract

“…Labeled probes were precipitated with sonicated salmon sperm DNA and E. coli tRNA, and were dissolved in 30 pl of formamide. In situ hybridization and detection of hybridized probes FISH was carried out for assignments of each gene as previously reported (9,10,11,12). The hybridization signals of bio-labeled probes were detected with avidin-FITC (Boehriger) and the metaphase chromosomes were counterstained with propidium iodide (PI) (1 ag/ml) (Sigma).…”

Section: Metaphase Chromosome Preparationsmentioning

confidence: 99%

“…Metaphase chromosomes were prepared by thymidine synchronization, bromodeoxyuridine (BrdU) release technique as previously reported (7,9). In brief, phytohemagglutinin-stimulated human peripheral blood lymphocytes from healthy donors were cultured at 37°C in RPMI1640 medium supplemented with 15% fetal calf serum (FCS).…”

Section: Metaphase Chromosome Preparationsmentioning

confidence: 99%

See 1 more Smart Citation

A Simple G-Banding Technique Adaptable for Fluorescent in situ Hybridization (FISH) and Physical Ordering of Human Renin (REN) and Catepsin E (CTSE) Genes by Multi-Color FISH.

Inazawa

Azuma

Ariyama

et al. 1993

Acta Histochem. Cytochem

Self Cite

View full text Add to dashboard Cite

We developed a simple technique for delineation of refined replication G-band in combination with fluorescent in situ hybridization (FISH). By using this system we localized three genes of pepsinogen C (PGC), renin (REN), and cathepsin E (CTSE) on 6p21.1, 1q32, and 1q32, respectively. Further, we determined the physical order of REN and CTSE on chromosome band 1q32 as cen-(1q32)-REN-CTSE-tel by multi-color FISH.The construction of refined physical and genetic maps is an indispensable step for the eventual sequencing of the entire human chromosome as well as for the " positional cloning" of target chromosome loci responsible for inherited genetic disorders and tumor suppressor genes. The use of in situ hybridization techniques on metaphase chromosomes has contributed to the construction of high-resolution cytogenetic maps (7,14). The technique with isotopelabeled probes is sensitive (8), although it has several disadvantages such as probe instability due to radiolysis of isotopes, long exposure times for detection of signals by autoradiographic procedures, and uncertain localization of signals captured by an emulsion overlay. The development of fluorescent in situ hybridization (FISH) has overcome some of these problems and this technique has been used as a standard tool for chromosomal mapping in recent years.Application of replication-banding techniques to FISH has enabled researchers to determine the precise location of numerous DNA loci directly on banded chromosomes (2, 5, 9, 15). Further, the development of multi-color FISH has strongly enhanced cytogenetic resolution in the ordering of closely located DNA loci on metaphase and interphase chromosomes.We describe here a simple high-resolution G-banding technique adaptable for FISH and further describe the physical mapping of pepsinogen C (PGC), renin (REN) and cathepsin E (CTSE) genes by this technique. MATERIALS AND METHODS Metaphase chromosome preparationsMetaphase chromosomes were prepared by thymidine synchronization, bromodeoxyuridine (BrdU) release technique as previously reported (7,9). In brief, phytohemagglutinin-stimulated human peripheral blood lymphocytes from healthy donors were cultured at 37°C in RPMI1640 medium supplemented with 15% fetal calf serum (FCS). After 48 hr incubation, thymidine was added to the medium at a final concentration of 300 pug/ml and incubation was continued for 16 hr. The cells were rinsed twice in RPMI1640 and then resuspended in supplemented RPMI1640 medium containing BrdU (25 ag/ml) (Sigma) for 6.0 hr. During the last 0.5 hr of this treatment, the cells were exposed to 0.02 ag/ml colcemid (Welcome). They were then harvested and resuspended in 0.075 M KCL at room temperature for 30 min. After fixation in 3 : 1 methanol : acetic acid, slides were prepared by the air-dry method, and fixed at 65°C for 5 hr.

show abstract

“…In situ hybridization and rinsing was carried out according to the protocol described by Takahashi et al (1989Takahashi et al ( , 1990) with slight modification (Inazawa et al, 1991). The hybridization mixture consisted of 0.5 ~g biotinlabeled cDNA, 50~ formamide, 2 • SSC, 10 ~ bovine serum albumin (BSA), and 20~o dextran sulfate (final concentration).…”

Section: Preparation Of High Resolution R-banded Chromosomesmentioning

confidence: 99%

Assignment of the human cytochrome P-450 nifedipine oxidase gene (CYP3A4) to chromosome 7 at band q22.1 by fluorescencein situ hybridization

et al. 1992

Self Cite

View full text Add to dashboard Cite

SummaryWe have used a full length cDNA clone (2.2 kb) for the human cytochrome P-450 nifedipine oxidase (CYP3A4) enzyme as a probe to determine its chromosome localization by fluorescence in situ hybridization. CYP3A4 was mapped on R-banded human prometaphase chromosomes, and the precise localization of CYP3A4 on chromosome 7 was further confirmed by a delineation of G-banded pattern on the same prometaphase chromosomes through a combination of UV-filter. We assigned CYP3A4 to chromosome 7 at q22.1.

show abstract

Assignment of the human granulocyte colony-stimulating factor receptor gene (CSF3R) to chromosome 1 at region p35–p34.3

Cited by 46 publications

References 10 publications

Deletion mapping on chromosome 1p in well-differentiated gastric cancer

Deletion mapping on chromosome 1p in well-differentiated gastric cancer

A Simple G-Banding Technique Adaptable for Fluorescent in situ Hybridization (FISH) and Physical Ordering of Human Renin (REN) and Catepsin E (CTSE) Genes by Multi-Color FISH.

Assignment of the human cytochrome P-450 nifedipine oxidase gene (CYP3A4) to chromosome 7 at band q22.1 by fluorescencein situ hybridization

Contact Info

Product

Resources

About