Assisted ion transfer at micro-ITIES supported at the tip of micropipettes

Shao, Yuanhua; Osborne, Murray D.

doi:10.1016/0022-0728(91)85297-3

Cited by 166 publications

(128 citation statements)

References 14 publications

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“…Simple ion transfer of an ion such as TEA + at the µITIES array results in a steady-state current on the forward sweep and a peak-shaped current on the reverse sweep. The asymmetric voltammograms are the result of radial and linear diffusion profiles during the forward and reverse scans, respectively [38,52,53]. from radial diffusion to the interface from the aqueous phase and the peak-shaped reverse response is due to linear diffusion of the analyte molecules, from the organic phase held within the membrane micropores, back to the aqueous phase.…”

Section: Influence Of Myoglobin On Tea + Transfer At the µIties Arraymentioning

confidence: 99%

Electrochemical behaviour of myoglobin at an array of microscopic liquid–liquid interfaces

O'Sullivan

Arrigan

2012

Electrochimica Acta

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Electrochemistry at liquid-liquid interfaces, or at interfaces between two immiscible electrolyte solutions (ITIES), provides a basis for the non-redox detection of biological molecules, based on iontransfer or adsorption processes. The electroactivity of myoglobin at an array of micron-sized liquidorganogel interfaces was investigated. The µITIES array was patterned with a silicon membrane consisting of an array of eight pores with radii of ~12.8 µm and a pore to pore separation of ~400 µm.Using cyclic voltammetry at the ITIES, the protein was shown to adsorb at the interface and facilitate the transfer of the organic phase electrolyte anions to the aqueous side of the interface. The electrochemical current response was linear with concentration in the range of 1 -6 µM, with corresponding surface coverage of 10 -50 pmol cm -2 . The reverse peak currents was found to be proportional to the voltammetric scan rate, indicating a desorption process. The detection of the protein was only possibly when the pH of the aqueous phase solution was below the pI of the protein. The steady-state simple ion transfer behaviour of tetraethylammonium cation was decreased on the forward sweep, providing a qualitative indication of the presence of adsorbed protein at the interface.Increasing the ionic strength of the aqueous phase resulted in enhanced peak currents, possibly due to aggregation of protein precipitates in the aqueous solution. UV/Vis absorbance spectroscopy was used 1 ISE Member.2 | P a g e to investigate the effects of various aqueous electrolyte solutions on the structure of the protein, and it was shown that at low pH the protein is at least partially denatured. These results provide the basis for label-free detection of myoglobin at the ITIES.

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Section: Influence Of Myoglobin On Tea + Transfer At the µIties Arraymentioning

confidence: 99%

Electrochemical behaviour of myoglobin at an array of microscopic liquid–liquid interfaces

O'Sullivan

Arrigan

2012

Electrochimica Acta

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“…7 109 Unfortunately, the debate was not helped by the use of vocabulary borrowed from the field of classical electrode kinetics and the mechanisms proposed were referred to as E, EC, CE, etc. In order to clarify this ambiguous situation, a new terminology has been introduced 110 and is illustrated in Fig. 9.…”

Section: Facilitated Ion Transfermentioning

confidence: 99%

Charge Transfer across Liquid—Liquid Interfaces

Girault¹

1993

Modern Aspects of Electrochemistry

108

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“…The typical example that can be mentioned here is the facilitated ion-transfers of alkali metal cations by crown ether derivatives. 9 Shao et al 10 put forward various mechanisms for these facilitated ion transfers, involving complexation in the organic phase, in the aqueous phase or at the interface. Finally, electron transfer reactions across the ITIES can be studied when suitable redox couples are dissolved in both phases.…”

Section: Principles and Experimental Arrangementsmentioning

confidence: 99%

“…However, in the 1980s, miniaturised ITIES were introduced by Girault and co-workers, 10,[12][13][14][15][16] first in the form of ITIES formed at the mouths of micropipettes and then in the form of ITIES formed at single or arrays of microholes drilled into polymer sheets and other materials. 17,18 The formation and properties of these µITIES, and subsequently introduced nanoITIES, 19 20, 21 will not be discussed here other than when they have been employed for studies of protein electrochemistry.…”

Section: Principles and Experimental Arrangementsmentioning

confidence: 99%

Voltammetry of proteins at liquid–liquid interfaces

Arrigan

2013

Annu. Rep. Prog. Chem., Sect. C: Phys. Chem.

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Assisted ion transfer at micro-ITIES supported at the tip of micropipettes

Cited by 166 publications

References 14 publications

Electrochemical behaviour of myoglobin at an array of microscopic liquid–liquid interfaces

Electrochemical behaviour of myoglobin at an array of microscopic liquid–liquid interfaces

Charge Transfer across Liquid—Liquid Interfaces

Voltammetry of proteins at liquid–liquid interfaces

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