Association analysis between variants in KITLG gene and litter size in goats

An, Xiaopeng; Hou, Jinxing; Gao, Teyang; Yue, Lei; Song, Yuxuan; Wang, J.G.; Cao, Binyun

doi:10.1016/j.gene.2014.12.058

Cited by 16 publications

(13 citation statements)

References 23 publications

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“…The SNPs of the PRLR gene regulated by bta-miR-302a associated with litter size in goats were analyzed (An et al, 2015b). The genetic polymorphisms of KITLG gene were explored, and the results indicated that three SNPs may play an important role in litter size (An et al, 2013b;Wang et al, 2017). Otherwise, some candidate genes, such as FTH1, GH, and SAA, were significantly associated with high litter size in Jining Grey goats (Feng et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

A novel 12 bp deletion within goat <i>LHX4</i> gene significantly affected litter size

et al. 2018

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Abstract. The LIM homeobox transcription factor 4 (LHX4) gene plays a critical role in regulating the development of the pituitary and the secretion of growth hormone (GH) and prolactin (PRL) associated with reproduction. Thus this gene may affect litter size. Herein, the aim of this study is to detect the novel insertion/deletion (indel) within the LHX4 gene as well as to test its association with litter size in 1149 Shaanbei white cashmere goats. Herein, a novel 12 bp indel (NC_030823.1:g.60001011_60001022delGGGGAGGAGGGG) was firstly found, which was located in the first intron. Meanwhile, three genotypes were detected in Shaanbei white cashmere goats, and the allelic frequencies of I and D were 0.593 and 0.407, respectively. Interestingly, the genotype distributions between mothers of single-lamb (n = 895) and multi-lamb (n = 254) groups within Shaanbei white cashmere goats were significantly different, implying that the 12 bp indel might affect the litter size. Furthermore, the association analysis was carried out to find out that the 12 bp indel was significantly associated with litter size in the analyzed goat population (P < 0.05). The litter sizes of genotype DD and ID individuals were superior to those of genotype II (P < 0.05). These findings suggest that this locus could be considered as a genetic marker for goat breeding, enriching the research category of functional genome of goats.

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Section: Discussionmentioning

confidence: 99%

A novel 12 bp deletion within goat <i>LHX4</i> gene significantly affected litter size

et al. 2018

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“…AR is well-known for its roles in male reproduction and has been recently revealed to be essential for normal female fertility, including follicle health, development, and ovulation (Walters et al 2010). Moreover, four miRNAs ( miR-136 , miR-101 , miR-148a , and miR-9-5p ) co-regulated KITLG , which is a strong candidate gene related to litter size in goats (An et al 2015).…”

Section: Resultsmentioning

confidence: 99%

Comparative mRNA and miRNA expression in European mouflon (Ovis musimon) and sheep (Ovis aries) provides novel insights into the genetic mechanisms for female reproductive success

Yang

Cao

et al. 2018

Heredity

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Prolific breeds of domestic sheep (Ovis aries) are important genetic resources due to their reproductive performance, which is characterized by multiple lambs per birth and out-of-season breeding. However, the lack of a comprehensive understanding of the genetic mechanisms underlying the important reproductive traits, particularly from the evolutionary genomics perspective, has impeded the efficient advancement of sheep breeding. Here, for the first time, by performing RNA-sequencing we built a de novo transcriptome assembly of ovarian and endometrial tissues in European mouflon (Ovis musimon) and performed an mRNA-miRNA integrated expression profiling analysis of the wild species and a highly prolific domestic sheep breed, the Finnsheep. We identified several novel genes with differentially expressed mRNAs (e.g., EREG, INHBA, SPP1, AMH, TDRD5, and ZP2) between the wild and domestic sheep, which are functionally involved in oocyte and follicle development and fertilization, and are significantly (adjusted P-value < 0.05) enriched in the Gene Ontology (GO) terms of various reproductive process, including the regulation of fertilization, oogenesis, ovarian follicle development, and sperm-egg recognition. Additionally, we characterized 58 differentially expressed miRNAs and 210 associated target genes that are essential for the regulation of female reproduction cycles through specific regulatory networks [e.g., (miR-136, miR-374a, miR-9-5p)-(EREG, INHBA)]. Furthermore, our integrated mRNA and miRNA expression profiling analysis elucidated novel direct and indirect miRNA/mRNA causal regulatory relationships related to the reproductive traits of the Ovis species. This study provides in-depth insights into the genomic evolution underlying the reproductive traits of the Ovis species and valuable resources for ovine genomics.

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“…In the current study, nine SNPs in six genes were strongly linked with sheep litter size, confirming their genetic roles in regulating sheep litter size. Previous research found two SNPs in KIT [9] and ten SNPs in KITLG were significantly associated with goat litter size [10,11,12,13]. A mutation in ADAMTS1 was associated with pig litter size [19].…”

Section: Discussionmentioning

confidence: 99%

“…Two single nucleotide polymorphisms (SNPs) in KIT were linked with goat litter size [9]. Similarly, ten loci in KITLG were significantly associated with goat litter size [10,11,12,13]. ADAMTS1 and NOG were crucial for ovulation [14,15,16,17,18] and a mutation in ADAMTS1 was notably linked with litter size in a new Qingping female line [19].…”

Section: Introductionmentioning

confidence: 99%

Association of Polymorphisms in Candidate Genes with the Litter Size in Two Sheep Breeds

Yuan

Zhang

et al. 2019

Animals

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Simple SummaryIncreasing litter size is critical for the intensive sheep production system. Genetic marker-assisted selection (MAS) based on proven molecular indicators could enhance the efficacy of sheep selection with improving litter size traits. Many single nucleotide polymorphisms (SNPs) linked with litter size in Hu sheep and Small-tailed Han sheep have been identified. However, they usually explain a small portion of genetic variation and additional genetic markers linked with litter size have not been found. The present study investigated the potential SNPs in ten genes as candidate markers for improved litter size in sheep. As a result, nine SNPs in six out of ten genes can be served as useful genetic markers to improve the selection of litter size since they are significantly associated with litter size either in Hu sheep or Small-tailed Han sheep. Further, a combined haplotypes analysis of the two loci (LIFR: g.35862868C>T and LIFR: g.35862947G>T) revealed that H2H3 (CTTT) combined haplotypes had the largest litter size than the rest combined haplotypes and more than those with either mutation alone in Small-tailed Han sheep. Our knowledge is essential to implement the MAS in sheep and further to increase the profitability in the sheep industry.AbstractHu sheep and Small-tailed Han sheep are the most widely raised and most famous maternal sheep breeds in China, which are known for precocious puberty, perennial oestrus and high fecundity (1–6 lambs each parity). Therefore, it is crucial to increase litter size of these two breeds for intensive sheep industry. The objective of this study was to identify potential genetic markers linked with sheep litter size located at ten genes. This study collected blood sample of 537 Hu sheep and 420 Small-tailed Han sheep with litter size of first parity. The average litter sizes in Hu sheep and Small-tailed Han sheep were 2.21 and 1.93. DNA-pooling sequencing method was used for detecting the potential single nucleotide polymorphisms (SNPs) in ten genes related to follicle development and female reproduction. SNPscan® was used for individually genotyping. As a result, a total of 78 putative SNPs in nine out of ten candidate genes (except NOG) were identified. In total, 50 SNPs were successfully genotyped in Hu sheep and Small-tailed Han sheep. After quality control, a total of 42 SNPs in Hu sheep and 44 SNPs in Small-tailed Han sheep were finally used for further analysis. Association analysis revealed that nine SNPs within six genes (KIT: g.70199073A>G, KITLG: g.124520653G>C, ADAMTS1: g.127753565T>C, ADAMTS1: g.127754640G>T, NCOA1: g.31928165C>T, NCOA1: g.32140565G>A, LIFR: g.35862868C>T, LIFR: g.35862947G>T and NGF: g.91795933T>C) were significantly associated with litter size in Hu sheep or Small-tailed Han sheep. A combined haplotypes analysis of the two loci (LIFR: g.35862868C>T and LIFR: g.35862947G>T) revealed that H2H3 (CTTT) combined haplotypes had the largest litter size than the rest combined haplotypes and more than those with either mutation alone in Sma...

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Association analysis between variants in KITLG gene and litter size in goats

Cited by 16 publications

References 23 publications

A novel 12 bp deletion within goat <i>LHX4</i> gene significantly affected litter size

A novel 12 bp deletion within goat <i>LHX4</i> gene significantly affected litter size

Comparative mRNA and miRNA expression in European mouflon (Ovis musimon) and sheep (Ovis aries) provides novel insights into the genetic mechanisms for female reproductive success

Association of Polymorphisms in Candidate Genes with the Litter Size in Two Sheep Breeds

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Association analysis between variants in KITLG gene and litter size in goats

Cited by 16 publications

References 23 publications

A novel 12 bp deletion within goat &lt;i&gt;LHX4&lt;/i&gt; gene significantly affected litter size

A novel 12 bp deletion within goat &lt;i&gt;LHX4&lt;/i&gt; gene significantly affected litter size

Comparative mRNA and miRNA expression in European mouflon (Ovis musimon) and sheep (Ovis aries) provides novel insights into the genetic mechanisms for female reproductive success

Association of Polymorphisms in Candidate Genes with the Litter Size in Two Sheep Breeds

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A novel 12 bp deletion within goat <i>LHX4</i> gene significantly affected litter size

A novel 12 bp deletion within goat <i>LHX4</i> gene significantly affected litter size