Association between bacterial strain type and host biomarkers in Clostridium perfringens infected goats

Khan, Mumtaz Ali; Durrani, A. Z.; Khan, Sher Bahadar; Khan, Muhammad Arif; Sheikh, Ali Ahmad; Khan, Noor; Prince, Kashif; Ullah, Naimat; Khan, Azmat Ullah

doi:10.1016/j.micpath.2017.09.059

Cited by 5 publications

(6 citation statements)

References 47 publications

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“…Other researchers identified C. perfringens type A, B, C and D in enterotoxaemic sheep, lambs, cattle, and calves respectively [13,16,17]. The highest contamination noticed after C. perfringens type A was type D, B and C [14,18]. C. perfringens type A, D and E were isolated from cow meat which was same with the study conducted by Miyashiro et al [19].…”

Section: Area and Month Wise Distribution Of Different Serotypes Of Csupporting

confidence: 62%

“…Alpha toxin is produced by all types of C. perfringens. C. perfringens type A is frequently found in intestine and feces of different mammalian species such as cattle, sheep and goats [13,14]. Previous studies have reported the prevalence of C. perfringens type A as 54.3% in Japan (Miki et al 2008), 20.4 % in beef samples and 16.6 % in lamb meat samples in USA [15].…”

Section: Area and Month Wise Distribution Of Different Serotypes Of Cmentioning

confidence: 99%

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The prevalence of Clostridium perfringens in retail meat of Mardan, Pakistan

Khan¹,

Khan²,

Khan³

et al. 2020

Turk J Vet Anim Sci

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The most prevailing bacteria in distribution is Clostridium perfringens (C. perfringens) which is ranked 1st among fatal diseases in small ruminants. This rapidly growing food borne pathogen is present in water, food, soil, air, and humans and animals intestinal tract. In the present study, a cross-sectional study was performed to sort out the prevalence of C. perfringens types estimation in various retail meats and areas of Mardan district, Pakistan. 600 fresh meat samples (equally from cows, sheep and goats) were collected from retail shops. C. perfringens was diagnosed in various types of meat however highest prevalence was found in goats 174/200 (87%) followed by sheep 103/200 (51.5%) while the least prevalence was in cow meat 89/200 (44.5%). All obtained isolates were further genotyped through multiplex PCR indicating 93/174 (53.45%) C. perfringens type A, 16/174 (9.19%) type B, 3/174 (1.72%) type C and 62/174 (35.63%) type D in goat isolates. 53/103 (51.46%) C. perfringens type A, 11/103 (10.68%) type B, 3/103 (2.91%) type C and 36/103 (34.95%) type D in sheep isolates. 73/89 (82.02%) C. perfringens type A, 9/89 (10.11%) type D and 7/89 (7.86%) type E in cow isolates. Out of 89 cow meat isolates 4 (4.49%) carried cpe gene, 16 (17.98%) contained cpb2 gene and 1 (1.12%) carried both cpe and cpb2 genes. In 103 sheep meat isolates, 2 (1.94%) carried cpe gene, 8 (7.77%) carried cpb2 gene and 3 (2.91%) carried both cpe and cpb2 genes. In 174 goat's meat isolates, 3 (1.72%) carried cpe gene, 18 (10.34%) carried cpb2 gene while (1.72%) carried both cpe and cpb2 genes. While analyzing prevalence in months and area wise, no significance was observed through chi-square test. It indicates that this pathogen is prevalent in various meat sources in Mardan and could be a threat to public health.

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Section: Area and Month Wise Distribution Of Different Serotypes Of Csupporting

confidence: 62%

Section: Area and Month Wise Distribution Of Different Serotypes Of Cmentioning

confidence: 99%

The prevalence of Clostridium perfringens in retail meat of Mardan, Pakistan

Khan¹,

Khan²,

Khan³

et al. 2020

Turk J Vet Anim Sci

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“…Non-significant changes were observed in packed cell volume and platelet count. Similarly biochemical analysis showed a significant increase in liver enzymes, serum creatinine, total bilirubin, blood urea and glucose in diseased goats [169]. In sheep, mean leukocyte count and packed cell volume was significantly higher in infected than control animals.…”

Section: Clinical Pathologymentioning

confidence: 88%

The Challenges of Diagnosis and Control of Enterotoxaemia Caused by <i>Clostridium perfringens</i> in Small Ruminants

Pawaiya¹,

Kumaresan²,

Gangwar³

et al. 2020

AiM

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“…Pathogenic strains of C. perfringens were identified having more than 104-107 CFU/g colony count on blood agar (Kalender et al 2005). C. perfringens type D isolates were confirmed by PCR using genomic DNA isolated from the pathogenic strains, cpa and etx specific primers F-5-TGC TAA TGTTAC TGC CGT TGA TAG-3; R-5-TGC TAA TGT TAC TGC CGT TGA TAG-3 and F-5-ATT AAA ATC ACA ATC ATT CAC TTG-3; R-5-CTT GTG AAG GGA CAT TAT GAG TAA-3 (Khan et al, 2017).…”

Section: Bacterial Culturesmentioning

confidence: 99%

“…Reported incidence rate of enterotoxemia in small ruminants is 2-8%, while the case fatality rate may reach 100% (Radostits, 2006). A number of factors including improper vaccination schedule or low-quality vaccine, low hygienic condition, no colostrum fed, fatty animals and low quality of feeding and watering management (Veschi et al, 2006;Khan et al, 2017) may cause the outbreaks of enterotoxaemia. To control this malady, it is necessary to provide quality vaccination and proper feeding (Metre, 2010).…”

Section: Introductionmentioning

confidence: 99%