Association between biofilm formation and susceptibility to antibiotics in Staphylococcuslentus isolated from urinary catheterized patients.

Abstract

Staphylococcuslentus is a coagulase negative gram positive cocci recognized as opportunistic pathogens and rarely biofilm forming and have many virulence factors,but recently causes nosocomial and community infections. Biofilm formation S. lentus may be associated with ability to resistant antibiotics lead to increase in mortality rate due to difficult in eradicate infections To evaluate the biofilm forming capacity in S.lentus and its susceptibility to antibiotics by using phenotypic and genotypic assay. Twenty eight biofilm bacteria among of them S. lentus were isolated and identified from urine catheterized patients who were hospitalized in different department of four Iraqi hospitals (Al-Diwaniyah teaching,Al- Hilla teaching,Al Qassim and Al Hashimiyah hospitals) S. lentus were examined for detection biofilm formation by detecting icaA gene intercellular adhesion gene which express adhesion factor to form biofilm in staphylococci by using PCR method and tested for antimicrobial susceptibility testing by disc diffusion method and VITEK2 system CLSI guidelines. Three isolates of S. lentus revealed the ability 100% to form biofilm phenotypicallywhich contained icaA gene with clearly antibiotics resistance (100% ) to each penicillin,Carbencillin,Gentamicin,Tobramycin,Oxacillin,Vancomycin,Clindamycin and Ciprofloxacin and (0%) to Azithromycin.icaA genes present in Staphylococcuslentus and responsible for biofilm formation which consider as indicator and the biofilm formation is a strong cause of multidrug resistance in bacteria.