Association between BMP15 Gene Polymorphism and Reproduction Traits and Its Tissues Expression Characteristics in Chicken

Abstract

BMP15 (Bone morphogenetic protein 15) is an oocyte-secreted growth factor required for ovarian follicle development and ovulation in mammals, but its effects on reproduction in chickens are unclear. In this study, the association between BMP15 polymorphisms and reproduction traits were analyzed, and its expression characteristics in different tissues were explored in LaiWu Black chickens. Three single nucleotide polymorphisms (SNPs) were identified in four hundred LaiWu Black chickens. One SNP (NC_006091.3:g.1773T>C) located in exon 2 which was significantly associated with egg weight at first egg (EWFE) (P = 0.0389), was novel. Diplotypes based on the three SNPs were found to be significantly associated with egg weight at age of 43W (EW43) (P = 0.0058). The chickens with H3H3 diplotype had their first egg 0.57 days later than chickens with H5H5 diplotype and 1.21 days-3.96 days earlier than the other five diplotype chickens. The egg production at age of 43W (E43), egg production at age of 46W (E46) and egg production at age of 48W (E48) for chickens with H3H3 diplotype were the highest among all the chickens, and the E48 of chickens with H3H3 diplotype had 11.83 eggs higher than chickens with H1H5 diplotype. RT-qPCR results showed that the expression level of BMP15 gene in ovarian follicle was in the order of 4 mm>6 mm -8 mm> 15 mm -19 mm> 23 mm -29 mm > 33 mm -34 mm in diameter. The mRNA level in follicles of 4 mm and 6–8 mm in diameter were significantly higher than that in the other follicles (P<0.01). In the same week, the highest mRNA level was found in the ovary, and it was significantly different from that found in the liver and oviduct (P<0.01). Our results indicate that BMP15 plays a vital role in the development of ovary and follicles, especially in the development of primary follicles. H3H3 may be an potential advantageous molecular marker for improving reproduction traits in chickens.

Transcriptome analysis reveals key genes and pathways associated with egg production in Nandan-Yao domestic chicken

“…The correlation analysis showed that the polymorphic loci of g310 A > g, g181 A > T, and g159 C > T were all correlated with the number of eggs in Beijing-You chicken population of different weeks (P < 0.05) [10]. Han HX et al (2015) studied the relationship between BMP15 gene polymorphism and reproductive traits of LaiWu Black chicken and identi ed three single nucleotide polymorphisms (SNPs) [11]. Sex hormone regulation also affects the development of sex organs and egg production.…”

Evolution and conservation of Characidium sex chromosomes

“…nuclear receptor subfamily 4 group A member 1 ( nr4a1 ; Abdou et al, 2013) and retinoic acid receptor, gamma b ( rargb ), closely mapped in LG22. Other important genes related to gonad development, bone morphogenetic protein 15 ( bmp15; Han et al, 2015) and cytochrome P450, family 26, subfamily b, polypeptide 1 ( cyp26b1 ; Saba et al, 2014) were found in LG16, while the estrogen receptor 2b gene ( esr2b ; Delalande et al, 2015) in zebrafish LG13.…”

Genotype frequency distributions of 28 SNP markers in two commercial lines and five Chinese native chicken populations

“…Based on multiple previous reports [17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33], we have chosen 14 characteristic SNP markers strongly associated with growth traits or carcass traits (P < 0.05) including rs13687128, rs317360945, rs14734533, rs314127605, NC_ 006092.4: g.25657391 T > A, NT_464126.1: g.66916G > A, rs314901473, rs316142388, rs314403820, rs14202565, rs16438236, rs13939265, rs15620544, rs16434462 and 14 characteristic SNP markers with strongly association with egg production traits (P < 0.05) including NC_006127.4: g.24021190G > A, rs16349546, NC_006096.4: g22545152 G > C, rs16486559, rs317328077, rs14714701, rs14085822, rs13905010, rs15602813, rs14491030, rs14699480, rs14652932, rs315420959 and rs313187645 for genotype frequency analysis. The specific information of the SNP markers is given together with their genetic background and corresponding traits in Tables 1 and 2.…”

“…To characterize the genetic variations and genetic relationships among different populations using DNA markers, a total of 28 identified SNP markers, including 14 growth or carcass traits associated loci and 14 egg laying related genome positions, were selected from the previous reports [17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33]. By using the MassArray technology, an Agena Bioscience MassARRAY System which is capable of efficiently genotyping tens to hundreds of SNPs with high accuracy, we detected the genotype frequency distributions of the 28 SNP markers in seven populations including four lowly selected as well as one moderately selected Sichuan native chicken populations, one commercial broiler line and one commercial layer line [34,35].…”