Association between cagA and vacA genotypes and pathogenesis in a Helicobacter pylori infected population from South-eastern Sweden

Karlsson, Anneli; Ryberg, Anna; Dehnoei, Marjan Nosouhi; Borch, Kurt; Monstein, Hans‐Jürg

doi:10.1186/1471-2180-12-129

Cited by 31 publications

(32 citation statements)

References 52 publications

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“…129) The proportions of Western CagA species containing one, two, and three EPIYA-C segments are approximately 60–70%, 20–30%, and <5%, respectively. 58) In contrast to EPIYA-C, the EPIYA-D segment rarely duplicates in East Asian CagA species.…”

Section: Caga Polymorphism In Pro-oncogenic Actionmentioning

confidence: 96%

Structure and function of <i>Helicobacter pylori</i> CagA, the first-identified bacterial protein involved in human cancer

Hatakeyama

2017

Proceedings of the Japan Academy. Ser. B: Physical and Biologic

187

143

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Chronic infection with Helicobacter pylori cagA-positive strains is the strongest risk factor of gastric cancer. The cagA gene-encoded CagA protein is delivered into gastric epithelial cells via bacterial type IV secretion, where it undergoes tyrosine phosphorylation at the Glu-Pro-Ile-Tyr-Ala (EPIYA) motifs. Delivered CagA then acts as a non-physiological scaffold/hub protein by interacting with multiple host signaling molecules, most notably the pro-oncogenic phosphatase SHP2 and the polarity-regulating kinase PAR1/MARK, in both tyrosine phosphorylation-dependent and -independent manners. CagA-mediated manipulation of intracellular signaling promotes neoplastic transformation of gastric epithelial cells. Transgenic expression of CagA in experimental animals has confirmed the oncogenic potential of the bacterial protein. Structural polymorphism of CagA influences its scaffold function, which may underlie the geographic difference in the incidence of gastric cancer. Since CagA is no longer required for the maintenance of established gastric cancer cells, studying the role of CagA during neoplastic transformation will provide an excellent opportunity to understand molecular processes underlying “Hit-and-Run” carcinogenesis.

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Section: Caga Polymorphism In Pro-oncogenic Actionmentioning

confidence: 96%

Structure and function of <i>Helicobacter pylori</i> CagA, the first-identified bacterial protein involved in human cancer

Hatakeyama

2017

Proceedings of the Japan Academy. Ser. B: Physical and Biologic

187

143

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“…The vacA gene, present in all strains, covers two different regions, the “s” region (s1 and s2) and the “m” region (m1 and m2) [7]. Another relevant marker of pathogenicity is the cagA gene, present in about 60-70% of the H. pylori strains.…”

Section: Introductionmentioning

confidence: 99%

Gene polymorphism of interleukin 1 and 8 in chronic gastritis patients infected with Helicobacter pylori

Neto

Rasmussen

Lábio

et al. 2014

J Venom Anim Toxins incl Trop Dis

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Background: Epidemiological investigations have indicated that Helicobacter pylori induces inflammation in the gastric mucosa regulated by several interleukins. The genes IL1B and IL8 are suggested as key factors in determining the risk of gastritis. The aim of this paper was to evaluate the association of gene polymorphism of interleukin-1 and interleukin-8 with chronic gastrits in H. pylori infected patients. A total of 60 patients underwent endoscopic procedure. Biopsy samples were collected for urease test, histopathological and molecular exams. The DNA of theses samples was extracted for detection of H. pylori and analysis of the genes mentioned above. Patients with gastritis had a higher frequency of H. pylori-positive samples.

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“…Their findings showed that two or more cagA EPIYA-C motifs are related to atrophy in gastric mucosa [19].…”

Section: Discussionmentioning

confidence: 99%

“…Vacuolating cytotoxin A (vacA), cytotoxin-associated gene A (cagA) protein, and urease enzyme are the most important virulence factors of H. pylori. Recent studies have focused on investigating the relationship between virulence factors and clinical condition [6][7][8]. CagA protein is present 60%-80% in H. pylori strains and is generally accompanied by VacA [9].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of H. pylori in gastric biopsy specimen in the southeastern region of Turkey

Bilman¹,

Özdemir

Baysal

et al. 2016

J Infect Dev Ctries

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Introduction: Helicobacter pylori is a Gram-negative, microaerophilic bacterium that colonizes human gastric mucosa. Gastric ulcer, duodenal ulcer, chronic atrophic gastritis, mucosa-associated lymphoid tissue lymphoma, and stomach adenocarcinoma are associated with H. pylori as the etiological agent. Cytotoxin-associated gene A (cagA), which is one of the most important virulence factors of H. pylori, encodes a 120-145 kDa protein.The prevalence of cagA genes shows differences in H. pylori infections based on geographical area, and cagA-positive H. pylori strains play an important role in pathogenesis of gastric carcinoma. Methodology: The aim of this study was to detect the prevalence of cagA and vacA genes in H. pylori isolates in adult patient groups in the southeastern region of Turkey. The presence of H. pylori was investigated in gastric biopsy specimens using the culture method, and polymerase chain reaction (PCR) analysis was performed to detect the presence of the cagA and vacA s1 genes. Results: H. pylori was detected in 65% (84/129) of patients who had gastrointestinal complaints. The number of vacA s1 and cagA genes of isolates were 44 (74.5%) and 31 (52.5%), respectively. Conclusions: H. pylori infection in southeastern region of Turkey with are comparable to those in developed countries. Patients with cagA-and vacA-positive H. pylori have a higher risk of severe inflammation and atrophy and should therefore be monitored for the development of gastric cancer.

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Association between cagA and vacA genotypes and pathogenesis in a Helicobacter pylori infected population from South-eastern Sweden

Cited by 31 publications

References 52 publications

Structure and function of <i>Helicobacter pylori</i> CagA, the first-identified bacterial protein involved in human cancer

Structure and function of <i>Helicobacter pylori</i> CagA, the first-identified bacterial protein involved in human cancer

Gene polymorphism of interleukin 1 and 8 in chronic gastritis patients infected with Helicobacter pylori

Prevalence of H. pylori in gastric biopsy specimen in the southeastern region of Turkey

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