Association between cytokine gene polymorphisms and squamous cell lung cancer depending on the duration of smoking in men

Abstract

Background. Squamous cell lung cancer (SCLC) is the most common form of lung cancer among men smokers. Mediators and products of inflammation can contribute to the initiation of carcinogenesis in smokers. The aim of study is to investigate the association between IL1B(rs1143634, rs16944), IL1RN (rs2234663), TNFA (rs1800629), IL6 (rs1800795), IL10 (rs1800896) genes and SCLC depending on the duration of smoking in men.
 Materials and methods. We studied 324 patients with SCLC and 168 healthy men smokers. The typing of the IL1RN(rs2234663) gene polymorphism has analyzed by polymerase chain reaction (PCR). Detection of the IL1B (rs1143634) and IL6 (rs1800795) polymorphisms have performed by restriction fragment length analysis, as a restriction enzyme TaqI was used. The IL1B (rs16944), TNFA(rs1800629) and IL10 (rs1800896) genes polymorphisms have determined through TaqMan-real-time PCR.
 Results. The study showed, the age, duration of smoking and the number of cigarettes smoked per day were the main risk factors for SCLC in men (p < 0.0001). The IL6 -174G allele was detected an additional risk factor of SCLC at a smoking duration of less than 35 years (OR = 1.68; 95 % CI: 1.12-2.51; pcor = 0,04). No association of IL1B (rs1143634, rs16944), IL1RN (rs2234663), TNFA (rs1800629), and IL10 (rs1800896) genes with the risk of SCLC in men was identified.
 Conclusion. The duration of smoking and rs1800795 IL6 gene polymorphism may influence on the susceptibility to SCLC in men. Our results can be useful in understanding the molecular mechanisms of development of SCLC.

Role of IL-1β rs1143634 (+3954C>T) polymorphism in cancer risk: an updated meta-analysis and trial sequential analysis

“…Figure 1 outlines the complete study selection process in this meta-analysis. Forty-four studies 17 , 25 – 67 were selected from 970 primary studies acquired from the searched databases following the eligibility criteria. Comprehensive screening of the titles, abstracts and full texts for each study was conducted to include or exclude the studies.…”

“… 34 , 53 This SNP did not show any association with NSCLC and small cell lung cancer in men. 29 , 35 , 41 For CRC, only polymorphism carriers among the Chinese Han population showed an increased risk, 56 but other studies reported a negative association. 44 , 64 Patients with MM carrying CT and TT alleles of the IL-1β +3954C>T polymorphism exhibited improved survival rates and survival conditions compared with the CC allele carriers.…”

Increased Frequencies of the ‒174G and ‒572C IL6 Alleles in Populations of Indigenous Peoples of Siberia Compared to Russians

“…It has been shown that their carriers are more likely to suffer from liver diseases [16] and have an increased risk of tuberculosis [15,17]. Allele -174G is considered a risk factor for squamous cell lung cancer and chronic obstructive pulmonary disease [18], cancer and fundic atrophy of the stomach [19], ovarian cancer [20], intrauterine infection of the fetus [21], development of cerebral arteriovenous malformation [22] and is associated with the risk of developing type 2 diabetes [23,24]. Allele -572С has also been associated with ischemic stroke in indigenous West African men [25].…”