Association between DNA ploidy and micronucleus frequency in chronic smokers and impact of smoking cessation

Cruz, Beatriz Serralheiro da; Schwarzmeier, Lígia Ângelo Tavares; Carvalho, Beatriz Fernanda; Alves, Mônica Ghislaine Oliveira; Carta, Celina Faig Lima; Balducci, Ivan; Scholz, Jaqueline; Almeida, Janete Días

doi:10.1177/0960327121991904

Cited by 5 publications

(2 citation statements)

References 24 publications

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“…The latter are extracellular cytoplasmic bodies that are formed during anaphase from chromosomal fragments. Several studies showed that there is a correlation between the frequency of MN and severity of the genotoxic damage, these being further used for grading the lesion [26,27]. Therefore, MN count is regarded as an indicator of chromosomal aberrations and is useful in detecting early-stage carcinogenesis [28,29].…”

Section: Introductionmentioning

confidence: 99%

Early Diagnosis of Oral Mucosal Alterations in Smokers and E-Cigarette Users Based on Micronuclei Count: A Cross-Sectional Study among Dental Students

Pop

Coroș

Stoica

et al. 2021

IJERPH

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The presence of micronuclei in oral epithelial cells is considered a marker of genotoxicity, which can be identified using exfoliative cytology. The aim of this study was to investigate cytotoxic damage through the evaluation of micronuclei in the oral mucosa of smokers and e-cigarette users compared to nonsmokers. We obtained smears from the buccal mucosa of 68 participants divided in 3 groups (smokers, e-cigarette users and nonsmokers), which were further processed with Papanicolaou stain. The frequencies of micronuclei and micronucleated cells were recorded and statistically analyzed at a level of significance of p < 0.05. The mean micronuclei values per 1000 cells were 3.6 ± 1.08 for smokers, 3.21 ± 1.12 for e-cigarette users and 1.95 ± 1.05 for nonsmokers. The mean values of micronucleated cells per 1000 cells were 2.48 ± 0.91 for smokers, 2.39 ± 1.07 for e-cigarette users and 1.4 ± 0.68 for nonsmokers. Smokers and e-cigarette users had significantly higher values of micronuclei and micronucleated cells compared to nonsmokers, but there were no significant differences between smokers and e-cigarette users. We concluded that the micronuclei count can be used as an early indicator for alterations of oral mucosa and exfoliative cytology represents an accessible tool which could be applied for mass screening.

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Section: Introductionmentioning

confidence: 99%

Early Diagnosis of Oral Mucosal Alterations in Smokers and E-Cigarette Users Based on Micronuclei Count: A Cross-Sectional Study among Dental Students

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Coroș

Stoica

et al. 2021

IJERPH

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“…We read the manuscript recently published by Cruz et al 1 in the Human and Experimental Toxicology titled "Association between DNA ploidy and micronucleus frequency in chronic smokers and impact of smoking cessation" with much interest. In this article, the authors detected high frequencies of micronucleated cells in chronic smokers when compared to control group.…”

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confidence: 99%

Are cytogenetic assays in exfoliated cells able to predict the development of oral cancer in chronic smokers?

et al. 2021

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Is micronucleus assay a useful marker in gingiva, tongue, and palate for evaluating cytogenetic damage induced by chemical, physical, and biological agents in vivo? A systematic review with meta‐analysis

Pinto,

Takeshita,

Renno

et al. 2024

J of Applied Toxicology

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The present systematic review (SR) aims to evaluate manuscripts in order to help further elucidate the following question: is the micronucleus assay (MA) also a useful marker in gingiva, tongue, and palate for evaluating cytogenetic damage in vivo? A search was performed through the electronic databases PubMed/Medline, Scopus, and Web of Science, all studies published up to December 2023. The comparisons were defined as standardized mean difference (SMD), and 95% confidence intervals (CIs) were established. Full manuscripts from 34 studies were carefully selected and reviewed in this setting. Our results demonstrate that the MA may be a useful biomarker of gingival tissue damage in vivo, and this tissue could be a useful alternative to the buccal mucosa. The meta‐analysis analyzing the different sites regardless of the deleterious factor studied, the buccal mucosa (SMD = 0.69, 95% CI, − 0.49 to 1.88, p = 0.25) and gingiva (SMD = 0.31, 95% CI, − 0.11 to 0.72, p = 0.15), showed similar results and different outcome for the tongue (SMD = 1.19, 95% CI, 0.47 to 1.91, p = 0.001). In summary, our conclusion suggests that the MA can be a useful marker for detecting DNA damage in gingiva in vivo and that this tissue could be effective site for smearing.

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Association between DNA ploidy and micronucleus frequency in chronic smokers and impact of smoking cessation

Cited by 5 publications

References 24 publications

Early Diagnosis of Oral Mucosal Alterations in Smokers and E-Cigarette Users Based on Micronuclei Count: A Cross-Sectional Study among Dental Students

Early Diagnosis of Oral Mucosal Alterations in Smokers and E-Cigarette Users Based on Micronuclei Count: A Cross-Sectional Study among Dental Students

Are cytogenetic assays in exfoliated cells able to predict the development of oral cancer in chronic smokers?

Is micronucleus assay a useful marker in gingiva, tongue, and palate for evaluating cytogenetic damage induced by chemical, physical, and biological agents in vivo? A systematic review with meta‐analysis

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