Association between estrogen receptor α polymorphisms and equol production, and its relation to bone mass

Katsuyama, Hironobu; Arii, Masayuki; Tomita, Masafumi; Hidaka, Kazuo; Watanabe, Yoko; Tamechika, Yoshie; Okuyama, Toshiko; Fushimi, Shigeko; Maeda, Noriko; Higashimura, Tsutomu; Fukunaga, Masao; Saijoh, Kiyofumi

doi:10.3892/ijmm_00000194

Cited by 4 publications

(4 citation statements)

References 24 publications

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“…According to Do et al ( 2007 ), the daily intake of in natura soybean grains is 2.5 ± 4.9 g ( n = 708 ). In Asian countries—the largest consumers of soybean—the daily intake of soybean and soy-related foods is 23.0 ± 18.2 g (Toyomura and Kono, 2002 ; Do et al, 2007 ; Katsuyama et al, 2009 ). Thus, the amount of Ni in soybean grains found in this study is considered safe and does not pose a threat to human health if direct consumption of grain is taking into account.…”

Section: Discussionmentioning

confidence: 99%

Hidden Nickel Deficiency? Nickel Fertilization via Soil Improves Nitrogen Metabolism and Grain Yield in Soybean Genotypes

et al. 2018

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Nickel (Ni)—a component of urease and hydrogenase—was the latest nutrient to be recognized as an essential element for plants. However, to date there are no records of Ni deficiency for annual species cultivated under field conditions, possibly because of the non-appearance of obvious and distinctive symptoms, i.e., a hidden (or latent) deficiency. Soybean, a crop cultivated on soils poor in extractable Ni, has a high dependence on biological nitrogen fixation (BNF), in which Ni plays a key role. Thus, we hypothesized that Ni fertilization in soybean genotypes results in a better nitrogen physiological function and in higher grain production due to the hidden deficiency of this micronutrient. To verify this hypothesis, two simultaneous experiments were carried out, under greenhouse and field conditions, with Ni supply of 0.0 or 0.5 mg of Ni kg−1 of soil. For this, we used 15 soybean genotypes and two soybean isogenic lines (urease positive, Eu3; urease activity-null, eu3-a, formerly eu3-e1). Plants were evaluated for yield, Ni and N concentration, photosynthesis, and N metabolism. Nickel fertilization resulted in greater grain yield in some genotypes, indicating the hidden deficiency of Ni in both conditions. Yield gains of up to 2.9 g per plant in greenhouse and up to 1,502 kg ha−1 in field conditions were associated with a promoted N metabolism, namely, leaf N concentration, ammonia, ureides, urea, and urease activity, which separated the genotypes into groups of Ni responsiveness. Nickel supply also positively affected photosynthesis in the genotypes, never causing detrimental effects, except for the eu3-a mutant, which due to the absence of ureolytic activity accumulated excess urea in leaves and had reduced yield. In summary, the effect of Ni on the plants was positive and the extent of this effect was controlled by genotype-environment interaction. The application of 0.5 mg kg−1 of Ni resulted in safe levels of this element in grains for human health consumption. Including Ni applications in fertilization programs may provide significant yield benefits in soybean production on low Ni soil. This might also be the case for other annual crops, especially legumes.

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Section: Discussionmentioning

confidence: 99%

Hidden Nickel Deficiency? Nickel Fertilization via Soil Improves Nitrogen Metabolism and Grain Yield in Soybean Genotypes

et al. 2018

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“…These results are consistent with the experimental evidence reported previously. 26,27 The differentiation of osteoblasts was assessed via ALP activity, an early marker of osteoblast differentiation. 28 After 3 days of treatment, the ALP activity of MC3T3-E1 cells was low because the cells were still in the proliferation stage.…”

Section: Discussionmentioning

confidence: 99%

Differentially expressed genes and signalling pathways are involved in mouse osteoblast-like MC3T3-E1 cells exposed to 17-β estradiol

Shang

Sun

et al. 2014

Int J Oral Sci

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Oestrogen is essential for maintaining bone mass, and it has been demonstrated to induce osteoblast proliferation and bone formation. In this study, complementary DNA (cDNA) microarrays were used to identify and study the expression of novel genes that may be involved in MC3T3-E1 cells' response to 17-β estradiol. MC3T3-E1 cells were inoculated in minimum essential media alpha (α-MEM) cell culture supplemented with 17-β estradiol at different concentrations and for different time periods. MC3T3-E1 cells treated with 10−8 mol⋅L−1 17-β estradiol for 5 days exhibited the highest proliferation and alkaline phosphatase (ALP) activity; thus, this group was chosen for microarray analysis. The harvested RNA was used for microarray hybridisation and subsequent real-time reverse transcription polymerase chain reaction (RT-PCR) to validate the expression levels for selected genes. The microarray results were analysed using both functional and pathway analysis. In this study, microarray analysis detected 5 403 differentially expressed genes, of which 1 996 genes were upregulated and 3 407 genes were downregulated, 1 553 different functional classifications were identified by gene ontology (GO) analysis and 53 different pathways were involved based on pathway analysis. Among the differentially expressed genes, a portion not previously reported to be associated with the osteoblast response to oestrogen was identified. These findings clearly demonstrate that the expression of genes related to osteoblast proliferation, cell differentiation, collagens and transforming growth factor beta (TGF-β)-related cytokines increases, while the expression of genes related to apoptosis and osteoclast differentiation decreases, following the exposure of MC3T3-E1 cells to α-MEM supplemented with 17-β estradiol. Microarray analysis with functional gene classification is critical for a complete understanding of complementary intracellular processes. This microarray analysis provides large-scale gene expression data that require further confirmatory studies.

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“…Because of the important roles of estrogen in the differentiation of tooth-forming cells [25], polymorphisms in ESRα and ESRβ may influence the occurrence of DF. Although previous research has reported ESR allele frequencies in different populations [26][27][28], the role of ESR gene polymorphisms in relation to DF has been unclear. Thus, we subsequently hypothesized that ESR gene polymorphic variations would be associated with DF status.…”

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confidence: 99%

Association of Dental Fluorosis with Polymorphisms of Estrogen Receptor Gene in Chinese Children

Zhang

Wang

et al. 2010

Biol Trace Elem Res

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Dental fluorosis (DF) is one of the important performances of endemic fluorosis. Some studies indicated that estrogen receptor (ESR) gene polymorphisms were associated with bone metabolism-related diseases. Therefore, it is possible that the variation in ESR genotypes will be associated with DF status. A case-control study was conducted among children aged 8-12 years with (n = 75) or without (n = 165) DF in China to investigate the relationship between ESR gene polymorphisms and DF. Gene polymorphisms were genotyped using the PCR-RFLP procedure. Children carrying R allele of ER RsaI had significantly increased risk of DF (Odds ratio (OR) = 1.821; 95% confidence interval (CI), 1.013-3.274) compared to children carrying r allele of ER RsaI in endemic fluorosis villages. For children with high-loaded fluoride status, carrying X allele of ESRα XbaI had a significantly decreased risk of DF (OR = 0.542; 95% CI, 0.314-0.936) compared to carrying x allele. This study provides the first evidence of an association between polymorphisms in the ESR gene with DF in high-fluoride-exposed populations. Further studies are needed to confirm the association.

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Association between estrogen receptor α polymorphisms and equol production, and its relation to bone mass

Cited by 4 publications

References 24 publications

Hidden Nickel Deficiency? Nickel Fertilization via Soil Improves Nitrogen Metabolism and Grain Yield in Soybean Genotypes

Hidden Nickel Deficiency? Nickel Fertilization via Soil Improves Nitrogen Metabolism and Grain Yield in Soybean Genotypes

Differentially expressed genes and signalling pathways are involved in mouse osteoblast-like MC3T3-E1 cells exposed to 17-β estradiol

Association of Dental Fluorosis with Polymorphisms of Estrogen Receptor Gene in Chinese Children

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