Association between Estrogen, Vitamin D and Microrna17 Gene Polymorphisms and Periapical Lesions

Abstract

This study evaluated the association between polymorphisms in genes encoding estrogen receptors 1 (ESR1) and 2 (ESR2), vitamin D receptor (VDR) and in microRNA17 (which binds to ESR1 and VDR) with persistent apical periodontitis (PAP) after the endodontic treatment. We included 162 patients who completed endodontic treatment at least one year ago and presented apical periodontitis at the beginning of the root canal therapy. Clinical and radiographic exams were performed to evaluate the presence of PAP or healthy periradicular tissues (healed). Saliva samples were collected as a genomic DNA. The genotyping of ESR1 (rs2234693 and rs9340799), ESR2 (rs1256049 and rs4986938), VDR (rs739837 and rs2228570) and miRNA17 (rs4284505) were performed by real-time PCR. Chi-square test was used to the distribution of genotype and allele frequencies. Haplotype analysis was also performed. Eighty-nine patients were included in the “healed” group and 73 in the “PAP” group. No association was found between the allelic and genotypic polymorphisms studied and PAP (p>0.05). Haplotype analysis also did not demonstrated an association (p>0.05). In conclusion, the genetic polymorphisms in ESR1, ESR2, VDR and miRNA17 are not associated with PAP.

Association of microRNA 17 host gene variant (rs4284505) with susceptibility and severity of systemic lupus erythematosus

“…28 Accumulating evidence identified various genetic variants within the MIR17HG gene, including (rs4284505; g.91349218A>G), (rs17735387; g.91353800G>T), (rs1428; g.91354516C>A), (rs7336610; g.91352883C>T), and (rs72640333; g.91352673T>A) that are destined to be correlated with different disorders involving multiple myeloma, 13 breast cancer, 4 multiple sclerosis, 21 and periapical lesions. 20 Until now, to the best of our knowledge, this is the first study investigating the association of MIR17HG (rs4284505; A>G) variant with the development of SLE among a sample of Middle Eastern population. Our findings indicated that the A/G genotype revealed a protective effect against the development of SLE, and this protective effect remains significant after stratifying the patients according to the presence of lupus nephritis.…”

“…miRNAs are large family clusters of tiny noncoding RNAs that are implicated in genomic and epigenomic regulation. 20 The primary function of miRNAs is to target the designated mRNAs causing their instability and/or degradation, mediating their inhibitory effects. 28 Accumulating evidence identified various genetic variants within the MIR17HG gene, including (rs4284505; g.91349218A>G), (rs17735387; g.91353800G>T), (rs1428; g.91354516C>A), (rs7336610; g.91352883C>T), and (rs72640333; g.91352673T>A) that are destined to be correlated with different disorders involving multiple myeloma, 13 breast cancer, 4 multiple sclerosis, 21 and periapical lesions.…”

“…13 On the contrary, another study investigated the association of MIR17HG (rs4284505) variant with the risk of persistent apical periodontitis and observed no significant association of this variant under recessive and dominant models with the progression of the disease. 20 Also, we encountered a single study that revealed a protective effect of the MIR17HG rs4284505*A allele with the development of breast carcinoma among the Australian population. 4 These conflicting findings confirm the previous notion that the genetic variants often exert their effect in "a celltype-specific and context-dependent manner."…”

“…miRNAs are large family clusters of tiny noncoding RNAs that are implicated in genomic and epigenomic regulation 20 . The primary function of miRNAs is to target the designated mRNAs causing their instability and/or degradation, mediating their inhibitory effects 28 .…”

“…The most common SNP of this gene cluster is rs4284505 (G>A), which identified within the first intron of the MIR17HG. This variant has been associated with several diseases, 4,12,13,20,21 but its impact on SLE development and severity has not been assessed. In this sense, the authors were inspired to investigate the association of MIR17HG (rs4284505) variant with the development of SLE and with the available clinical and laboratory data in a sample of the Middle Eastern population.…”