Association between fine particulate matter and coronary heart disease: A miRNA microarray analysis

Guo, Jianhui; Xie, Xiaoxu; Wu, Jieyu; Yang, Ling; Ruan, Qishuang; Xu, Xingyan; Wei, Donghong; Wen, Yeying; Wang, Tinggui; Hu, Yuduan; Lin, Ya-Wen; Chen, Mingjun; Wu, Jiadong; Lin, Shuo; Li, Huangyuan; Wu, Siying

doi:10.1016/j.envpol.2022.120163

Cited by 7 publications

(2 citation statements)

References 52 publications

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“…Various epidemiological studies have demonstrated the correlation between PM exposure and morbidity and mortality related to respiratory and cardiovascular diseases [29,30]. The exact mechanism via which PM deteriorates health remains unclear; however, most studies suggest that the association between the generation of ROS and the consequent OS-induced plays a central role in generating PM-induced cytotoxicity [31].…”

Section: Discussionmentioning

confidence: 99%

Impact of Particulate Matter Exposure and Aerobic Exercise on Circulating Biomarkers of Oxidative Stress, Antioxidant Status, and Inflammation in Young and Aged Mice

Cho,

Roh

2023

Life

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Exposure to particulate matter (PM) and exercise training can have antagonistic effects on inflammatory responses and the balance between pro-oxidants and antioxidants in the body. However, the underlying mechanisms of these effects remain unclear. This study aimed to investigate the effects of PM exposure and aerobic exercise training on oxidative stress, antioxidant status, and inflammation in mice of different ages. Two groups of male C57BL/6 mice, comprising forty 1-month-old and forty 12-month-old mice, were exposed to either PM or exercise training or both for 8 weeks. PM exposure led to significantly higher 8-hydroxydeoxyguanosine (8-OHdG), malondialdehyde (MDA), interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α) levels (p < 0.05) and significantly lower superoxide dismutase (SOD) and catalase (CAT) activities (p < 0.05) in both age groups exposed to PM compared to the control groups. Conversely, aerobic exercise training led to significantly lower 8-OHdG, MDA, IL-1β, IL-6, and TNF-α levels (p < 0.05) and significantly higher SOD and CAT activities (p < 0.05) in both age groups receiving exercise training, compared to those exposed to PM. Moreover, young mice in the exercise training and PM group showed significantly lower 8-OHdG, MDA, and IL-1β levels (p < 0.05) and significantly higher SOD and CAT activities (p < 0.05) than young mice in the PM exposure group. However, these levels did not vary significantly between the group of old mice that either received exercise training or exposure to PM. Our results suggest that while PM exposure could cause pro-oxidant/antioxidant imbalances and inflammatory responses, regular aerobic exercise could ameliorate these negative effects, although these vary with age. Nevertheless, the antioxidant and anti-inflammatory effects of exercise were countered by PM exposure, especially in older mice.

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Section: Discussionmentioning

confidence: 99%

Impact of Particulate Matter Exposure and Aerobic Exercise on Circulating Biomarkers of Oxidative Stress, Antioxidant Status, and Inflammation in Young and Aged Mice

Cho,

Roh

2023

Life

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“…Studies have shown that airborne particulate matter may contribute to bronchial inflammation and affect immune regulation in lung injury [ 28 , 29 ]. The expression of miRNAs is altered after PM 2.5 exposure, and miR-4726-5p expression is elevated in response to PM 2.5 stimulation and affects the development of coronary heart disease [ 30 ]. Exposure of H9C2 cells to PM 2.5 results in the upregulation of miR-421 expression and downregulation of SIRT3 expression, triggering mitochondrial damage and consequent cardiovascular diseases [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA miR-212-5p Regulates the MEK/ERK Signaling Pathway by Targeting A-Raf proto-oncogene serine/threonine-protein kinase (ARAF) to Regulate Cowshed PM2.5-Induced NR8383 Apoptosis

Sun,

Jia

et al. 2023

Toxics

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Objective: To investigate the role of miR-212-5p-targeted ARAF during the apoptosis of rat alveolar macrophages induced by cowshed PM2.5. Methods: miRNA and related target genes and pathways were predicted using the KEGG, TargetScan, and other prediction websites. NR8383 macrophages were treated with cowshed PM2.5 to establish an in vitro lung injury model in rats; meanwhile, for the assessment of cell viability, apoptosis, intracellular calcium ions, and mitochondrial membrane potential in NR8383 cells, RT-qPCR was used to detect the expression of miR-212-5p and the target gene ARAF. Results: The bioinformatic analyses showed that miR-212-5p and ARAF were involved in PM2.5-associated cellular damage. Exposure to different concentrations (0 μg/mL, 60 μg/mL, 180 μg/mL, 300 μg/mL) with different durations (0 h, 12 h, 24 h, 48 h) of cowshed PM2.5 resulted in apoptosis, increased intracellular calcium ions, and decreased mitochondrial membrane potential. The miR-212-5p mimic group showed an up-regulation of Bax and cleaved Caspase 3 expression but decreased Bcl2 expression compared to the NC group, and overexpression of ARAF up-regulated the expression of p-MEK1/2 and p-ERK1/2 and simultaneously reversed the above phenomena. Conclusions: miR-212-5p targets ARAF to affect the cowshed PM2.5-induced apoptosis through the MEK/ERK signaling pathway, providing a potential target for relevant farming industry and pathology studies.

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Highly sensitive ECL detection of miRNA based on self-generated coreactant and target-induced catalyzed hairpin assembly

Wang,

Liu,

Zhang

et al. 2024

Analytica Chimica Acta

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Association between fine particulate matter and coronary heart disease: A miRNA microarray analysis

Cited by 7 publications

References 52 publications

Impact of Particulate Matter Exposure and Aerobic Exercise on Circulating Biomarkers of Oxidative Stress, Antioxidant Status, and Inflammation in Young and Aged Mice

Impact of Particulate Matter Exposure and Aerobic Exercise on Circulating Biomarkers of Oxidative Stress, Antioxidant Status, and Inflammation in Young and Aged Mice

MicroRNA miR-212-5p Regulates the MEK/ERK Signaling Pathway by Targeting A-Raf proto-oncogene serine/threonine-protein kinase (ARAF) to Regulate Cowshed PM2.5-Induced NR8383 Apoptosis

Highly sensitive ECL detection of miRNA based on self-generated coreactant and target-induced catalyzed hairpin assembly

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