Association between Genetic Polymorphisms of miR-1307, miR- 1269, miR-3117 and Breast Cancer Risk in a Sample of South East Iranian Women

Abstract

Introduction: MicroRNAs (miRNAs) play an essential role in the susceptibility and development of cancer cells. Objective: Examining the dependency of breast cancer risk with genetic polymorphisms of miR-1307, miR-1269, and miR-3117 in a sample of Iranian women (southeast region). Methods: The case-control study consisted of 520 individuals (260 diagnosed BC patients, 260 healthy individuals). The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used for genotyping of miR-1307 rs7911488, miR-1269 rs73239138, and miR-3117 (rs4655646 and rs7512692) polymorphisms. Results and Conclusion: This study provided evidence that miR-1307 rs7911488 polymorphism significantly reduced the risk of BC in heterozygous AG genotype, as well as dominant (AG+GG) genotype and G allele. A significant correlation was found between dominant (AA+AG) genotype, the A allele and protection against BC due to miR-1269 rs73239138 in the sample of study. In contrast, our findings suggested that AG genotype and G allele of miR-3117 rs4655646 polymorphism could increase BC's susceptibility among the southeastern Iranian females. The miR-3117 rs7512692 variant also increased the risk of BC in codominant, dominant and recessive models, as well as the T allele. The possible dependency of miR-1307, miR-1269, and miR-3117 variants with patients' clinicopathological characteristics and BC was also studied. It was concluded that there is a correlation between miR-3117 rs7512692 variant and tumor grade (p=0.031); also, a correlation between miR-1269 rs73239138 variant and progesterone receptor status (p=0.006). The current investigation revealed that miR-1307, miR-1269, and miR-3117 polymorphisms might play a crucial role in the Iranian population's vulnerability to BC.

Effect of MiR-375 Regulates YAP1 on the Invasion, Apoptosis, and Epithelial-Mesenchymal Transition of Cervical Cancer HeLa Cells

“…Abnormal expression of miRNA causes abnormal transcription and expression of its target genes, which provides important conditions for the occurrence and metastasis of malignant tumors [ 9 ]. With the continuous development of molecular biology technology in recent years, studies have found that a variety of miRNAs are abnormally expressed in cervical cancer [ 10 ], breast cancer [ 11 ], bladder cancer [ 12 ], and other malignant tumors, making miRNAs become new targets for targeted therapy of malignant tumors.…”

Impact of the HOTAIR (rs920778) and miR-3117 (rs7512692) variants on the Susceptibility and Clinicopathologic characteristics of patients with colorectal cancer

“…For the miR-3117 gene, the following sequences were identi ed: rs7512692-F: 5'-TGG CAG TTG CTG GTA CTC TT-3' and rs7512692-R: 5'-CTCAAGTCTCCTCCCCCATC-3'. for the variant rs4655646-F: 5´ TGG CAT GTG AGG AAA GTT GGA-3´ and rs4655646-R: 5´ AGATATTGGGCCTCTACCCGT-3´ [30].…”

“…Five microliters of each PCR product were subjected to digestion with 5U of the following restriction enzymes: The HOTAIR rs920778 (MspI) and miR-3117 rs7512692 (Hpy1881II) and rs4655646 (TaqI) (New England Biolabs, USA) were digested according to the manufacturer's instructions and separated in a 6% polyacrylamide gel. Genotypes were identi ed according to the methods described by Kashani et al ( 2021) and Sarabandi et al (2021). To ensure the quality of the genotyping processes, approximately 10% of the randomly selected samples were reprocessed, and the results were found to be 100% consistent.…”

miR-1269a and miR-1269b: Emerging Carcinogenic Genes of the miR-1269 Family

“…rs73239138 is a single nucleotide polymorphism located in the sequence of miR-1269a. miR-1269a rs73239138 is also associated with a reduced risk of breast cancer among women in southeastern Iran (Sarabandi et al, 2021). In gastric cancer and liver cancer cells, overexpressed miR-1269a can inhibit the apoptosis of gastric cancer cells.…”