Association between genetic variability and titre of Grapevine Pinot gris virus with disease symptoms

Abstract

The present work was carried out in order to verify the possible association between a new grapevine disease, characterized by leaf mottling and deformation, and the genetic variability and concentration of Grapevine Pinot gris virus (GPGV), a recently identified virus tentatively associated with the pathology. After vineyard surveys and the establishment of real-time qPCR assays, characterization of GPGV isolates and evaluation of GPGV titre were assessed in more than 100 samples of grapevine Glera, collected from plants regardless of whether or not they showed the symptomatology. Results showed that there was an important association between the GPGV variants and manifestation of the symptoms, and that grapevines with symptoms harboured significantly higher GPGV titre than symptomless vines. Moreover, an interesting relationship among the phylogenetic clustering of the isolates originating from plants with symptoms and some epidemiological characteristics of the disease was found. The current study confirmed the role of GPGV in the emergent disease characterized by grapevine leaf mottling and deformation.

Viral Diversity in Autochthonous Croatian Grapevine Cultivars

“…Since all GPGV-infected vines were multiply infected, it was not possible to connect disease symptoms to GPGV alone. According to Bertazzon et al (2016), expression of symptoms is not correlated only with GPGV variants, but also with virus populations; symptomatic vines have significantly higher virus populations when compared with symptomless vines.…”

With or Without You: Altered Plant Response to Boron-Deficiency in Hydroponically Grown Grapevines Infected by Grapevine Pinot Gris Virus Suggests a Relation Between Grapevine Leaf Mottling and Deformation Symptom Occurrence and Boron Plant Availability

“…Symptoms associated with GPGV infection include delayed budburst, stunted shoots, leaf distortion and mottling, increased berry acidity and poor yield (Saldarelli et al, 2015;AWRI, 2018). Symptoms are most distinct at the beginning of the season and are less apparent on late season growth, with infected plants reported to produce GLMD symptomless shoots and leaves from June onward (Bertazzon et al, 2017). The association between the symptomatology and the presence of the virus is unclear, with several reports documenting a great number of symptomless grapevines hosting GPGV (Giampetruzzi et al, 2012;Saldarelli et al, 2013Saldarelli et al, , 2015Forte et al, 2014;Mavrič Pleško et al, 2014;Bianchi et al, 2015).…”

First report of Grapevine Pinot gris virus infecting grapevine in Brazil

“…Chardonnay (Eichmeier et al 2016b). Also, Bertazzon et al (2016b) showed that there was an important association between GPGV variants and symptom expression, and that grapevines with symptoms harboured a significantly higher GPGV titre than symptomless vines. In the present study, it was not possible to associate specific symptoms with GPGV infections due to the frequent presence of multiple viral infections in the analyzed grapevines.…”