Association betweenGPX5Gene Polymorphism and Selenium Concentration in Liver and Kidney of Wild Boars from West Pomerania Province, Poland

Abstract

The aim of the experiment was to detect polymorphism in the GPX5 gene and to determine associations between individual genotypes and selenium concentration in liver and kidney of wild boars from West Pomerania province in Poland. The polymorphism in GPX5 gene was detected using the PCR-RFLP method with specific primers and the restriction enzyme HinfI. Two different alleles of the GPX5 gene were identified -allele 1B (0.37) and 2B (0.63). The relationships between the GPX5 genotypes and concentrations of selenium in liver and kidney were analyzed, revealing statistically significant (P≤0.05) differences between wild boars carrying different GPX5 genotypes in both genders. Obtained results indicated that wild boars with the 2B2B genotype had the highest selenium concentration in livers and kidneys compared to 1B1B and 1B2B animals and these differences were statistically significant (P≤0.05).

Novel polymorphisms in GPX5 gene and their association with reproductive and productive traits in Lumsniang (Hampshire x Niang Megha) pigs under subtropical hilly region of Meghalaya

“…The genotypic frequencies of AA, AG and GG were 0.157, 0.478 and 0.365 and allelic frequencies of A and G were 0.396 and 0.604, respectively, in the studied breed. The frequency of 1B2B (AG) genotype obtained in the present study is in consonance with the findings of Terman et al (2016), who reported 1B2B genotype was the most frequent genotype with a frequency of 0.50 and the 1B1B genotype was least frequent (0.12) in wild boars. Likewise, Buske et al (2006) also found that farrowing, farrowing intervals, litter size at birth, litter size at weaning, average birth weight, average weaning weight; and body weight were recorded at monthly intervals.…”

“…Thus, genetic variations could be probably affected by artificial selection, because selection may significantly change the genotypic and allelic distribution of GPX5 gene. Terman et al (2016) also found that the investigated pig populations were not in Hardy-Weinberg equilibrium, which is compatible with our findings. In this study, a total of one SNP locus in GPX5 i.e., SNP-HinfI (g.1896A>G) detected in the Intron 1, and no mutation caused a change of amino acid (Fig.…”

“…1A). The PCR product of similar size has also been reported by Buske et al (2006) in commercial pig population (Large White x Landrace) and Terman et al (2016) found in wild boars by amplification of this intron of the GPX5. When these fragments were digested with a restrictionenzyme, Hinf1, revealed three band patterns (Fig.…”

“…GPX5 is a noncanonical selenium-independent GPX gene, which encodes an epididymis-specific enzyme in mice, rats, pigs, monkeys as well as in humans (Terman et al, 2016). GPX5 accounts for more than 95% of epididymal GPX mRNA and protein when combined with GPX3 (Brigelius-Flohé and Maiorino, 2013).…”

“…Barranco et al (2016) also observed the presence of GPX5 in seminal plasma and identified that boars with high GPX5 levels had higher farrowing rates and litter sizes than boars with low GPX5 levels. Moreover, Terman et al (2016) studied the relationship between GPX5 genotypes and selenium concentrations in the liver and kidneys of wild boars and their findings revealed the highest selenium concentrations in the liver and kidneys of wild boars with the 2B2B genotype as compared to 1B1B and 1B2B genotypes of animals.…”