Association between Ki67 Index and Clinicopathological Features in Colorectal Cancer

Pan, Li; Xiao, Zhitao; Braciak, Todd A.; Ou, Qing-Jan; Chen, Gong; Oduncu, Fuat

doi:10.1159/000450623

Cited by 33 publications

(28 citation statements)

References 20 publications

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“…Staining for Ki67 tissue expression was performed using the primary anti-Ki67 antibody (1:50, Tokyo, Japan). The Ki67 was calculated for each sample as the percentage of positively stained tumor cells among all counted tumor cells [20].…”

Section: Immunohistochemistry (Ihc)mentioning

confidence: 99%

Exosomal circRNA-100338 promotes hepatocellular carcinoma metastasis via enhancing invasiveness and angiogenesis

Huang

et al. 2020

J Exp Clin Cancer Res

292

198

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Background: Exosomes play crucial roles in regulating the crosstalk between normal and cancer cells in the tumor microenvironment, and in regulating cancer proliferation, migration and invasion through their cargo molecules. Methods: We analyzed the pro-invasiveness of exosomal circRNA-100,338 in HCC using the transwell invasion assay. The co-culture of human umbilical vein endothelial cells (HUVEC) and exosomes derived from HCC cell lines were used to evaluate the impact of HCC derived exosomes on HUVEC. Nude mice models were used to validate the findings in vitro. Clinically, quantitative RT-PCR was used to quantify the expression of serum exosomal circRNA-100,338 in HCC patients at both pre-surgery within one week and post-surgery within three weeks. Results: We aim to investigate the pro-invasive role of exosomal circRNA-100,338 in HCC metastasis. We for the first time demonstrated that circRNA-100,338 was highly expressed in both highly metastatic HCC cells and their secreted exosomes. The transwell invasion assay showed that the overexpression or knockdown of exosomal circRNA-100,338 significantly enhanced or reduced the invasive abilities of HCC cells. Subsequently, in vitro and in vivo assays showed that exosomal circRNA-100,338 affected the cell proliferation, angiogenesis, permeability, and vasculogenic mimicry (VM) formation ability of human umbilical vein endothelial cells (HUVEC), and tumor metastasis. Furthermore, we also observed that the persistent high expression of exosomal circRNA-100,338 in serum of HCC patients who underwent curative hepatectomy may be a risk indicator of pulmonary metastasis and poor survival. Conclusions: Our findings indicated that metastatic ability of HCC cells could be enhanced by transferring exosomal circRNA-100,338 to recipient HUVECs, which could affect proangiogenic activity by regulating angiogenesis.

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Section: Immunohistochemistry (Ihc)mentioning

confidence: 99%

Exosomal circRNA-100338 promotes hepatocellular carcinoma metastasis via enhancing invasiveness and angiogenesis

Huang

et al. 2020

J Exp Clin Cancer Res

292

198

View full text Add to dashboard Cite

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“…In the light of its clinical significance, the Ki67 labeling index has been widely used as a diagnostic approach for assessing a number of tumor types (26). Recently, Li et al suggested that a high Ki67 index can be regarded as an independent prognostic biomarker associated with clinicopathological features of patients with stage III and IV CRC (29).…”

Section: Discussionmentioning

confidence: 99%

Independent Correlation Between Ki67 Index and Circulating Tumor Cells in the Diagnosis of Colorectal Cancer

Yang

Jin

et al. 2017

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“…Proliferation index was assessed using Ki67 staining and percentage positive staining assessed. Patients were then graded as low or high using a cut‐off value of 30% . Additional clinical data was taken from pathological reports after resection.…”

Section: Methodsmentioning

confidence: 99%

The association between markers of tumour cell metabolism, the tumour microenvironment and outcomes in patients with colorectal cancer

Roseweir

Clark

McSorley

et al. 2019

Intl Journal of Cancer

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Tumour cell anaerobic metabolism has been reported to be a prognostic factor in colorectal cancer. The present study investigated the association between monocarboxylate transporter (MCT) 1, MCT 2, lactate dehydrogenase (LDH) 1 and LDH 5, the tumour microenvironment, and outcome in patients with colorectal cancer. A cohort of 150 patients with stage I-III CRC were utilised to assess tumour cell expression of MCT-1, MCT-2, LDH-1 and LDH-5 by immunohistochemistry. Expression levels were dichotomised and associations with tumour factors, the tumour microenvironment and survival analysed. Nuclear LDH-5 associates with poor prognosis (HR 1.68 95% CI 0.99-2.84, p = 0.050) and trends toward increased tumour stroma percentage (TSP, p = 0.125). Cytoplasmic MCT-2 also trends toward increased TSP (p = 0.081). When combined into a single score; nuclear LDH-5 + TSP significantly associated with decreased survival independent of stage (HR 2.61 95% CI 1.27-5.35, p = 0.009), increased tumour budding (p = 0.002) and decreased stromal T-lymphocytes (p = 0.014). Similarly, cytoplasmic MCT-2 + TSP significantly associated with decreased survival (HR 2.32 95% CI 1.31-4.11, p = 0.003), decreased necrosis (p = 0.039), and increased tumour budding (p = 0.004). The present study reports that the combination of TSP and nuclear LDH-5 was significantly associated with survival, increased tumour budding, and decreased stromal T-lymphocytes. This supports the hypothesis that increased stromal invasion promotes tumour progression via modulation of tumour metabolism. Moreover, MCT-2 and LDH-5 may provide promising therapeutic targets for patients with stromal-rich CRC.

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Association between Ki67 Index and Clinicopathological Features in Colorectal Cancer

Cited by 33 publications

References 20 publications

Exosomal circRNA-100338 promotes hepatocellular carcinoma metastasis via enhancing invasiveness and angiogenesis

Exosomal circRNA-100338 promotes hepatocellular carcinoma metastasis via enhancing invasiveness and angiogenesis

Independent Correlation Between Ki67 Index and Circulating Tumor Cells in the Diagnosis of Colorectal Cancer

The association between markers of tumour cell metabolism, the tumour microenvironment and outcomes in patients with colorectal cancer

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