Association between maternal diet, smoking, and the placenta MTHFR 677C/T genotype and global placental DNA methylation

Diaz-Garcia, Hector; Vilchis-Gil, Jenny; Castro-Cerritos, Karla V.; Rivera-Susunaga, Luis E.; Klünder-Klünder, Miguel; Granados-Riveron, Javier T.; Gómez-López, Jaqueline; López-Torres, Adolfo; Sánchez-Urbina, Rocío

doi:10.1016/j.placenta.2023.12.017

Cited by 1 publication

(1 citation statement)

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“…The up-regulation of 2′-deoxyguanosine during early pregnancy may reflect the increased demand for nucleotides required for the rapid cellular proliferation and growth that occurs in the early stages of embryonic development. 2′-deoxyguanosine, a nucleoside analog, is known for its role in nucleic acid metabolism and has been implicated in various biological processes, including DNA replication and repair, which is integral to nucleic acid metabolism, suggests a connection between early gestational metabolic changes and nucleotide requirements for embryonic development ( 28–30 ). However, the precise mechanisms underlying the association between Hyodeoxycholic acid and 2′-deoxyguanosine levels and early pregnancy in sows remain to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

Metabolomics reveals early pregnancy biomarkers in sows: a non-invasive diagnostic approach

Ren,

Zhang,

et al. 2024

Front. Vet. Sci.

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In an effort to enhance reproductive management and reduce non-productive periods in swine breeding, this study presents a novel, non-invasive metabolomics approach for the identification of early pregnancy biomarkers in sows. Utilizing an untargeted metabolomics approach with mass spectrometry analysis, we examined saliva samples from pregnant (n = 6) and non-pregnant control sows (n = 6, artificially inseminated with non-viable sperm). Our analysis revealed 286 differentially expressed metabolites, with 152 being up-regulated and 134 down-regulated in the pregnant group. Among these, three metabolites, namely Hyodeoxycholic acid, 2′-deoxyguanosine, and Thymidine, emerged as potential early pregnancy biomarkers. These biomarkers were further evaluated using targeted LC–MS/MS quantification and qualification, accompanied by ROC curve analysis. The study confirmed Hyodeoxycholic acid and 2′-deoxyguanosine as promising biomarkers for early pregnancy detection, offering potential for future implementation in swine production environments. This research establishes a robust theoretical foundation for the development of innovative molecular diagnostic techniques and explores new avenues for molecular genetic breeding and non-invasive diagnostics, ultimately enhancing fertility and productivity in sow herds.

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Section: Discussionmentioning

confidence: 99%