Association between miR-196a2 polymorphism and the development of hepatocellular carcinoma in the Egyptian population

Abstract

Background: Hepatocellular carcinoma (HCC) is one of the most prevalent cancers worldwide. Circulating microRNAs (miRNAs) are endogenous, small (17-25 nucleotides) non-coding RNAs that are overexpressed in many human cancers including HCC. Single-nucleotide polymorphisms (SNPs) of miRNAs play an important role in the pathogenesis of HCC. In our study, we aimed to evaluate the role of miR-196a2 rs11614913 polymorphism in the development of HCC. A total of 200 subjects, including 80 HCC patients, 60 patients with liver cirrhosis, and 60 healthy controls were selected. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was taken to determine miR-196a2 rs11614913 polymorphism. Results: The genotype distribution of the TC and CC, TC + CC genotypes, and the C allele were significantly higher in HCC patients than control and cirrhotic groups (P = 0.02, P = 0.005, and P = 0.003, respectively). Compared with the wild-type TT genotype, both the variant TC, CC, TC + CC genotypes were associated with an elevated risk of HCC (OR = 2.77, 95% CI = 1.27-6.04), (OR = 4.94, 95% CI = 1.74-14.07), (OR = 3.24, 95% CI = 1.55-6.78) respectively. Moreover, the C allele was correlated with an increased risk of HCC (OR = 2.30, 95% CI = 1.40-3.76) compared to the wide-type T allele. Also, there is no significant correlation between the different miR-196a2 genotypes and either the clinico-pathologic features of HCC or its aggressiveness. Conclusion: Our results suggest that the miR-196a2 rs11614913 polymorphism is associated with an increased risk of HCC in the Egyptian population.

Evaluation of diagnostic accuracy of serum calcium channel α2δ1 subunit in hepatocellular carcinoma-related cirrhosis

“…Since the liver cancer is one of the highest malignancies in Egypt and carries a bad prognosis, early diagnosis and management are essential [24].…”

Effect of miR-196a2 rs11614913 Polymorphism on Cancer Susceptibility: Evidence From an Updated Meta-Analysis

“…[9][10][11] MiR-196a2 is an important member of the miRNA-196 precursor family found in the homeobox (HOX) clusters region of the human genome. 12 An extensively studied miR-196a2 variant is rs11614913 (C > T), which is investigated in a plethora of cancers, including breast cancer, [13][14][15][16][17] gastric cancer, [18][19][20][21][22] hepatocellular carcinoma, [23][24][25] colorectal cancer, [26][27][28][29] lung cancer, [30][31][32] gynecological cancer, [33][34][35][36][37][38] prostate cancer, [39][40][41] and so on. Despite a large number of studies performed in almost all ethnic populations, the contribution of rs11614913 polymorphism to cancer risk is still inconclusive.…”

Association between miR-196a-2 Gene Polymorphism and Ovarian Cancer Prognosis in Egyptian Females

“…The CC genotype produced two fragments (125 and 24 bp), the TT homozygote produced one 149 bp fragment and the TC heterozygote produced three fragments (125, 149, and 24 bp). The experiment was performed in tripli¬cate (Gawish et al, 2020).…”

“…When it acts as an inhibitory factor of oncogenic molecules, it acts as a tumor suppressor and when it targets tumor suppressors, it acts as an oncogene (Chen et al, 2011). miR-196a-2 polymorphism has significant associations with various types of cancer, including breast, lung, esophageal, gastric, and hepatocellular cancer (Alshatwi et al, 2012;Hu et al, 2008;Tutar, 2014;Peng et al, 2010 andGawish et al, 2020). Carriers of the homozygote variant CC are more likely to develop gastric cancer compared with wild-type homozygote TT and heterozygote CT carriers and the C allele was significantly associated with lymph node metastasis of gastric cancer (Peng et al, 2010).…”