Association between miRNA‐146a rs2910164 (G/C) polymorphism with the susceptibility to chronic HBV infection and spontaneous viral clearance in an Iranian population

Abstract

Hepatitis B virus (HBV) infection is one of the clinical dilemmas in chronic liver diseases. MicroRNAs (miRNAs) are small noncoding RNA molecules that play an important role in the pathogenesis of liver diseases and single nucleotide polymorphisms (SNPs) in miRNA genes affect the clinical course of HBV infection. Previous studies have shown that miRNA‐146a rs2910164 polymorphism can be associated with the pathogenesis of liver diseases such as hepatocellular carcinoma. The present study investigated the association between miRNA‐146a rs2910164 polymorphism and susceptibility to HBV infection in an Iranian population. The study comprised 266 patients with chronic HBV infection, 172 patients with spontaneous viral clearance (SVC) after acute HBV infection, and 266 healthy control adjusted for sex and age. The genotyping of the miRNA‐146a rs2910164 polymorphism was performed by polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) method. Our data revealed that GG genotype and G allele of miRNA‐146a rs2910164 SNP is dominated (P < 0.001) in patients with chronic HBV infection (Odds ratio [OR] = 3.92; 95% confidence interval [CI] = 2.1‐7.32). miRNA‐146a rs2910164 polymorphism showed a statistically significant association (P < 0.001) between CC genotype and allele C with SVC (OR = 2.92; 95% CI = 1.56‐546). Our findings suggest miRNA‐146a SNP (C/G) in our population may be associated with the susceptibility to HBV infection and CC genotype is associated with SVC. Also, the GG genotype and G allele at miRNA‐146a rs2910164 is associated with chronic HBV infection in our population.

Aberrant expression of two miRNAs promotes proliferation, hepatitis B virus amplification, migration and invasion of hepatocellular carcinoma cells: evidence from bioinformatic analysis and experimental validation

“…(D) Quantification from C. The two-independent t-test was used to compare the difference of wound healing rate and cell number. ÃÃ P < 0.01; ÃÃÃ P < 0.001. to HBV infection and with spontaneous viral clearance (Khanizadeh et al, 2019). TP53 mutation, the most common mutation in HCC, affects the progression and prognosis of HCC (Long et al, 2019).…”

The Association between Four Common Polymorphisms in microRNA and Risk of Hepatocellular Carcinoma: An Updated Meta-Analysis

“…1 , after precluding 193 irrelevant studies as judged from their titles and/or abstracts, the remaining 36 articles were reviewed for a more detailed evaluation. Based on the exclusion criteria, 7 studies were excluded: 1 about a review ( 25 ), 3 for insufficient data to evaluate OR and 95%CI ( 26 – 28 ), 2 for control group with HCC ( 29 , 30 ), and 1 for case group uncontained HCC( 31 ). Consequently, 29 studies met the inclusion criteria in this meta-analysis.…”

Peer Review #1 of "Aberrant expression of two miRNAs promotes proliferation, hepatitis B virus amplification, migration and invasion of hepatocellular carcinoma cells: evidence from bioinformatic analysis and experimental validation (v0.2)"

“…In China, the persistent exacerbation of HBV amplification is the main determinant of HCC recurrence (Yanming et al 2019). A recent study showed that miR-146a SNP (C/G) is associated with the susceptibility to HBV infection and with spontaneous viral clearance (Khanizadeh et al 2019). TP53 mutation, the most common mutation in HCC, affects the progression and prognosis of HCC (Long et al 2019).…”