Association between miRNA Target Sites and Incidence of Primary Osteoarthritis in Women from Volga-Ural Region of Russia: A Case-Control Study

Abstract

Over the past decades, numerous studies on the genetic markers of osteoarthritis (OA) have been conducted. MiRNA targets sites are a promising new area of research. In this study, we analyzed the polymorphic variants in 3′ UTR regions of COL1A1, COL11A1, ADAMTS5, MMP1, MMP13, SOX9, GDF5, FGF2, FGFR1, and FGFRL1 genes to examine the association between miRNA target site alteration and the incidence of OA in women from the Volga-Ural region of Russia using competitive allele-specific PCR. The T allele of the rs9659030 was associated with generalized OA (OR = 2.0), whereas the C allele of the rs229069 was associated with total OA (OR = 1.43). The T allele of the rs13317 was associated with the total OA (OR = 1.67). After Benjamini-Hochberg correction, only rs13317 remained statistically significant. According to ethnic heterogeneity, associations between the T allele (rs1061237) with OA in women of Russian descent (OR = 1.77), the G allele (rs6854081) in women of Tatar descent (OR = 4.78), the C allele (rs229069) and the T allele (rs73611720) in women of mixed descent and other ethnic groups (OR = 2.25 and OR = 3.02, respectively) were identified. All associations remained statistically significant after Benjamini-Hochberg correction. Together, this study identified miRNA target sites as a genetic marker for the development of OA in various ethnic groups.

The Genetic Markers of Knee Osteoarthritis in Women from Russia

“…We found an association between the T allele of rs13317 in FGFR1 and the incidence of the total OA and knee OA in women from the Volga-Ural region of Russia. The G allele of rs6854081 in FGF2 was a risk factor for OA development for women of Tatar descent, the C allele of rs1061237 in COL1A1 was a risk factor for OA development for Russian women and the T allele of rs229069 in ADAMTS5 and the T allele of rs73611720 in GDF5 were risk factors for OA development for women of mixed descent [6]. Thus, several ethnospecific genetic markers of osteoarthritis have been identified, which makes it promising to continue research in this direction.…”

Polymorphic Variants of the <i>ADAMTS5</i> Gene as New Markers of Joint Hypermobility

“…Неизвестно, каким образом полиморфизм влияет на активность связывания микроРНК, поскольку сродственные микроРНК предсказаны только для аллелей G и С. Также не идентифицированы потенциальные микроРНК, обладающие сродством к полиморфному варианту rs9978597 [21]. Ранее нами был проведен поиск ассоциаций аллеля С локуса rs229069 с развитием ОА у 356 женщин из Республики Башкортостан и была выявлена ассоциация с ОА в общей выборке [22]. Исходя из этих данных, описанные полиморфные варианты гена ADAMTS5 являются маркерами патологии опорно-двигательного аппарата, однако необходимо дальнейшее их исследование с формированием более гомогенных групп сравнения по типу и фенотипу заболевания, а также этническим параметрам когорт.…”