Association between mitochondrial DNA haplotype compatibility and increased efficiency of bovine intersubspecies cloning

Yan, Hao; Zhou, Yan; Ma, Qiang; Jiao, Fei; Huang, Shu‐Zhen; Zeng, Fanyi; Zeng, Yi‐Tao

doi:10.1016/j.jcg.2010.12.003

Cited by 14 publications

(15 citation statements)

References 32 publications

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“…A haplotype-specific influence of mitochondrial metabolism on nuclear gene expression has been outlined for ageing, cancer, and other diseases [9] as well as for evolutionary processes [18,20,22,34]. Our study now indicates that cytoplasmatically mediated modifications of the nuclear epigenome should also be considered as possible regulators of these effects on downstream components of health [12,[29][30][31]. The previously elusive molecular mechanisms uncovered here are likely candidates to mediate the documented mito-nuclear effects on the expression of health and life history traits such as longevity and reproductive success.…”

Section: Discussionsupporting

confidence: 52%

“…Previous research has also found that variation in the mt genotype can be associated with variation in mtDNA copy number [57,58]. Suggestions that epigenetic nuclear variation may be related to mtDNA copy number per cell [28,29,59] may therefore be an attractive hypothesis to be tested in future research.…”

Section: Discussionmentioning

confidence: 96%

“…Mitochondrial function is largely dependent on the nuclear genome, first and foremost due to the fact that the vast majority of proteins present in mitochondria are nuclear-encoded. However, studies have also shown or suggested that mitochondria can influence nuclear gene expression and might be involved in the epigenetic regulation of the nuclear genome [9,12,17,[28][29][30][31][32]. For instance, depleting mitochondria in cultured human kidney cells caused changes in nuclear DNA methylation, an important bearer of epigenetic information [33].…”

Section: Introductionmentioning

confidence: 99%

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The Cytoplasm Affects the Epigenome in Drosophila melanogaster

et al. 2018

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Cytoplasmic components and their interactions with the nuclear genome may mediate patterns of phenotypic expression to form a joint inheritance system. However, proximate mechanisms underpinning these interactions remain elusive. To independently assess nuclear genetic and epigenetic cytoplasmic effects, we created a full-factorial design in which representative cytoplasms and nuclear backgrounds from each of two geographically disjunct populations of Drosophila melanogaster were matched together in all four possible combinations. To capture slowly-accumulating epimutations in addition to immediately occurring ones, these constructed populations were examined one year later. We found the K4 methylation of histone H3, H3K4me3, an epigenetic marker associated with transcription start-sites had diverged across different cytoplasms. The loci concerned mainly related to metabolism, mitochondrial function, and reproduction. We found little overlap (<8%) in sites that varied genetically and epigenetically, suggesting that epigenetic changes have diverged independently from any cis-regulatory sequence changes. These results are the first to show cytoplasm-specific effects on patterns of nuclear histone methylation. Our results highlight that experimental nuclear-cytoplasm mismatch may be used to provide a platform to identify epigenetic candidate loci to study the molecular mechanisms of cyto-nuclear interactions.

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

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The Cytoplasm Affects the Epigenome in Drosophila melanogaster

et al. 2018

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“…Previous reports provided evidence for oocyte mtDNA haplotype influences on SCNT efficiency in cattle [12,13,32]. Yan et al [14,33] demonstrated that donor-host mtDNA haplotype compatibility improved bovine SCNT efficiency; thus, homotypic SCNT blastocysts possess a more successful epigenetic symmetry pattern than heterotypic SCNT blastocysts. It is unclear in the present study whether the differential protein expression patterning was reflective of a donor-host mtDNA incompatibility.…”

Section: Discussionmentioning

confidence: 99%

Comparative Proteomic Analysis of Liver Mitochondrial Proteins Derived from Cloned Adult Pigs Reconstructed with Meishan Pig Fibroblast Cells and European Pig Enucleated Oocytes

Takeda

Tasai

Iwamoto³

et al. 2012

Journal of Reproduction and Development

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Abstract. Somatic cell nuclear transfer (SCNT) has been exploited in efforts to clone and propagate valuable animal lineages. However, in many instances, recipient oocytes are obtained from sources independent of donor cell populations. As such, influences of potential nuclear-cytoplasmic incompatibility, post SCNT, are largely unknown. In the present study, alterations in mitochondrial protein levels were investigated in adult SCNT pigs produced by microinjection of Meishan pig fetus fibroblast cells into enucleated matured oocytes (maternal Landrace genetic background). Mitochondrial fractions were prepared from liver samples by mechanical homogenization and differential centrifugation. Liver mitochondria were then subjected to two-dimensional difference gel electrophoresis (2-D DIGE). Protein expression changes were confirmed with a volume ratio greater than 2 fold (P < 0.05). 2-D DIGE analysis further revealed differential expression of three proteins between the Meishan (n=3) and Landrace (n=3) breeds. Differential expression patterns of 16 proteins were detected in SCNT pig liver tissue (n=3) when compared with Meishan control samples. However, none of the 16 proteins correlated with the three differentially expressed Meishan and Landrace liver mitochondrial proteins. In summary, alteration of mitochondrial protein expression levels was observed in adult SCNT pigs that did not reflect the breed difference of the recipient oocytes. Comparative proteomic analysis represents an important tool for further studies on SCNT animals.

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“…Previous reports provided evidence for the influence of oocyte mtDNA haplotype on SCNT efficiency in cattle [70][71][72]. The mitochondria transmitted by nuclear transfer may affect the phenotypic changes observed in cloned animals [73].…”

Section: Mitochondrial Proteomic Approach In Cloned Cattle and Pigsmentioning

confidence: 99%

Mitochondrial DNA transmission and confounding mitochondrial influences in cloned cattle and pigs

Takeda

2013

Reprod Medicine & Biology

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Although somatic cell nuclear transfer (SCNT) is a powerful tool for production of cloned animals, SCNT embryos generally have low developmental competency and many abnormalities. The interaction between the donor nucleus and the enucleated ooplasm plays an important role in early embryonic development, but the underlying mechanisms that negatively impact developmental competency remain unclear. Mitochondria have a broad range of critical functions in cellular energy supply, cell signaling, and programmed cell death; thus, affect embryonic and fetal development. This review focuses on mitochondrial considerations influencing SCNT techniques in farm animals. Donor somatic cell mitochondrial DNA (mtDNA) can be transmitted through what has been considered a ''bottleneck'' in mitochondrial genetics via the SCNT maternal lineage. This indicates that donor somatic cell mitochondria have a role in the reconstructed cytoplasm. However, foreign somatic cell mitochondria may affect the early development of SCNT embryos. Nuclear-mitochondrial interactions in interspecies/intergeneric SCNT (iSCNT) result in severe problems. A major biological selective pressure exists against survival of exogenous mtDNA in iSCNT. Yet, mtDNA differences in SCNT animals did not reflect transfer of proteomic components following proteomic analysis. Further study of nuclear-cytoplasmic interactions is needed to illuminate key developmental characteristics of SCNT animals associated with mitochondrial biology.

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Association between mitochondrial DNA haplotype compatibility and increased efficiency of bovine intersubspecies cloning

Cited by 14 publications

References 32 publications

The Cytoplasm Affects the Epigenome in Drosophila melanogaster

The Cytoplasm Affects the Epigenome in Drosophila melanogaster

Comparative Proteomic Analysis of Liver Mitochondrial Proteins Derived from Cloned Adult Pigs Reconstructed with Meishan Pig Fibroblast Cells and European Pig Enucleated Oocytes

Mitochondrial DNA transmission and confounding mitochondrial influences in cloned cattle and pigs

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