Association between Polymerization Degree of Apple Peel Polyphenols and Inhibition of Helicobacter pylori Urease

Abstract

Apple peel extracts and their fractions pooled according to their molecular size were prepared and evaluated for their inhibitory activity against Helicobacter pylori and Jack bean ureases. Urease Inhibitory effect of apple peel polyphenols (APPE) extracted from the Granny Smith variety was concentration-dependent and reversible. High molecular weight polyphenols (HMW) were more active against Helicobacter pylori and Jack bean ureases than low molecular weight polyphenols with IC50 values of 119 and 800 microg GAE/mL, respectively. The results suggest that monomeric compounds (mainly flavan-3-ols-and quercetin-O-glycosides) will not be implicated in the antiurease effect displayed by the apple peel polyphenolic extract. Thus, as a byproduct, apple peel is suitable for developing functional ingredients that could be useful for neutralizing an important Helicobacter pylori colonization factor.

The Gastrointestinal Tract as a Key Target Organ for the Health-Promoting Effects of Dietary Proanthocyanidins

“…Some studies suggest that PAC DP is a determining factor for urease inhibition or their protective effects on VacA-induced mucosal damage. LMW PAC fraction (mean DP of 3) derived from apple peel exhibited an urease inhibition fourfold lower than the high HMW fraction (mean DP of 9.5) (44). More recently, an aqueous extract of Peumus boldus Mol.…”

Optimization of Ultrasonic-Assisted Extraction of Phenolic Compounds from Apples

“…Additionally, extracts were spiked with known standards to confirm the identification. Some of them were tentatively identified by using literature data, like phloretin-2′-xyloglucoside (Tsao et al 2003;Alonso-Salces et al 2004;Pastene et al 2009;Balázs et al 2012;Jakobek et al 2013), quercetin-3-xyloside (Jakobek et al 2013), and pcoumaroylquinic acid (Tsao et al 2003;Jakobek et al 2013). Identified compounds are shown in Fig.…”

“…Many studies have associated apples and their nutrients with the positive influence on the human health. Bioactive polyphenols from apples have shown antiviral properties (Suárez et al 2010), the inhibition of Helicobacter pylory (Pastene et al 2009), and anticarcinogenic properties (Veeriah et al 2007). Due to many positive effects, apples are studied intensively.…”

Antielastase, antiurease and antioxidant activities of some 3,13-monohydroxy eicosanoic acid isomers

“…Many synthetic and natural apple polyphenols have shown inhibitory activity against urease activity. 38, 39 Xiao et al explained the urease inhibition activity of polyphenols by the ability of the hydroxyl group to form a complex with the nickel metal located at the active center of urease. 38 In this study, the urease inhibition activity of the monohydroxy C 20 acid isomers was found to increase dose dependently.…”