Association between SLC11A1 (NRAMP1) polymorphisms and susceptibility to tuberculosis in Chinese Holstein cattle

Liu, Kaihua; Zhang, Bin; Teng, Zhaochun; Wang, Youtao; Dong, Guodong; Xu, Cong; Qin, Bo; Song, Chunlian; Chai, Jun; Yang, Li; Shi, Xiaowen; Shu, Xianghua; Yi-fang, Zhang

doi:10.1016/j.tube.2016.11.003

Cited by 24 publications

(26 citation statements)

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“…The frequency of the alternative allele at this SNP was found to be highest in the Sahiwal cattle, thought to be more resistant to bTB ( Ameni et al, 2007 ; Vordermeier et al, 2012 ), and lowest in HF cattle, which seem to be more susceptible to bTB ( Ameni et al, 2007 ; Vordermeier et al, 2012 ). Interestingly, this SNP has previously been associated with resistance/susceptibility to bTB in Chinese Holstein cattle, where the presence of the alternative allele in the CG and GG genotypes appeared to confer resistance to M. bovis infection ( Liu et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

“…A previous study has identified single nucleotide polymorphisms (SNPs) in the bovine SLC11A1 gene that are associated with susceptibility to bTB (Cheng et al, 2015). A study in Taiwanese HF cattle identified SNPs in exon 4 and intron 4 that were associated with enhanced susceptibility to bTB (Cheng et al, 2015), while in a Chinese HF cattle population, associations with bTB susceptibility/resistance were found with SNPs in exon 11 and introns 5 and 9 (Liu et al, 2017). Additionally polymorphisms in the 3 UTR of the bovine SLC11A1 gene, that have been shown to cause changes to the lengths of two microsatellites (MS1 and MS2), have been identified in various breeds of cattle (Paixao et al, 2006;Vazquez-Flores et al, 2006;Hasenauer et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Genetic Variation in the Bovine SLC11A1 Gene, Its Influence on the Expression of NRAMP1 and Potential Association With Resistance to Bovine Tuberculosis

et al. 2020

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Bovine tuberculosis (bTB), caused by Mycobacterium bovis , is a chronic zoonotic disease where host genetics is thought to contribute to susceptibility or resistance. One of the genes implicated is the SLC11A1 gene, that encodes for the natural resistance-associated macrophage protein 1 (NRAMP1). The aim of this study was to identify SLC11A1 polymorphisms and to investigate any resulting functional differences in NRAMP1 expression that might be correlated with resistance/susceptibility to M. bovis infection. Sequencing of the SLC11A1 gene in cDNA isolated from Brown Swiss, Holstein Friesian, and Sahiwal cattle identified five single nucleotide polymorphisms (SNPs) in the coding region, but only one of these (SNP4, c.1066C>G, rs109453173) was present in all three cattle breeds and therefore warranted further investigation. Additionally, variations of 10, 11, and 12 GT repeats were identified in a microsatellite (MS1) in the SLC11A1 3′UTR. Measurement of NRAMP1 expression in bovine macrophages by ELISA showed no differences between cells generated from the different breeds. Furthermore, variations in the length of the MS1 microsatellite did not impact on NRAMP1 protein expression as analyzed by luciferase reporter assay. However, further analysis of the ELISA data identified that the presence of the alternative G allele at SNP4 was associated with increased expression of NRAMP1 in bovine macrophages. Since NRAMP1 has been shown to influence the survival of intracellular pathogens such as M. bovis through the sequestering of iron, it is possible that cattle expressing the alternative G allele might have an increased resistance to bTB through increased NRAMP1 expression in their macrophages.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of Genetic Variation in the Bovine SLC11A1 Gene, Its Influence on the Expression of NRAMP1 and Potential Association With Resistance to Bovine Tuberculosis

et al. 2020

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“…Polymorphism in the Slc11a1 gene (SLC11A1 in humans) is associated with susceptibility to infection by microbes in different hosts. These include Mycobacterium tuberculosis infection of humans, mice, pigs, and cattle [16]; infection of chickens and mice by S. enterica serovars Pullorum and Enteritidis [17]; and Brucella abortus infection of cattle [18]. The G169D substitution in the Slc11a1 protein, found in mouse strains frequently used in research (e.g., C57BL6/J and Glossary mRNA leader: the 5′ region of an mRNA up to the start codon of the associated coding region.…”

Section: The Macrophage Protein Slc11a1 Mediates Resistance To Several Intracellular Pathogensmentioning

confidence: 99%

How Pathogens Feel and Overcome Magnesium Limitation When in Host Tissues

Blanc‐Potard

Groisman²

2021

Trends in Microbiology

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Host organisms utilize nutritional immunity to limit the availability of nutrients essential to an invading pathogen. Nutrients may include amino acids, nucleotide bases, and transition metals, the essentiality of which varies among pathogens. The mammalian macrophage protein Slc11a1 (previously Nramp1) mediates resistance to several intracellular pathogens. Slc11a1 is proposed to restrict growth of Salmonella enterica serovar Typhimurium in host tissues by causing magnesium deprivation. This is intriguing because magnesium is the most abundant divalent cation in all living cells. A pathogen's response to factors such as Slc11a1 that promote nutritional immunity may therefore reflect what the pathogen 'feels' in its cytoplasm, rather than the nutrient concentration in host cell compartments. HighlightsNutritional immunity is a host defense mechanism that limits nutrient availability to an invading pathogen.

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“…Using a logistic regression approach was found that SLC11A1-SNP1, SLC11A1-SNP3, and SLC11A1-SNP5 were significantly associated with susceptibility/resistance to TB. Haplotype analysis showed that nine haplotypes were potentially resistant to TB (Liu et al 2017).…”

Section: Tuberculosismentioning

confidence: 99%

Resistencia y resiliencia a las enfermedades en las razas de rumiantes locales: un enfoque en América del Sur

et al. 2020

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La pérdida de variabilidad genética de interés ha tomado importancia no sólo la producción, sino también porque puede tener un efecto negativo en la epidemiología de las enfermedades animales. La actividad ganadera puede presentar considerables pérdidas económicas periódicas debido a la eliminación de animales, la reducción de la productividad, la falta de expresión del potencial genético de los animales, los costes de tratamiento, la asistencia laboral y profesional, el cierre del comercio debido a los obstáculos sanitarios y la competencia con los mercados extranjeros. Por lo tanto, la resistencia a las enfermedades es un atributo deseado en la producción ganadera y la salud animal puede estar limitando en los sistemas de producción de ganado. Existe cierta confusión en la terminología: la resistencia se define como la capacidad del huésped para ejercer cierto grado de control sobre el ciclo de vida del patógeno, mientras que la tolerancia define el impacto de la infección en el rendimiento animal. Un concepto muy cercano a la tolerancia es la resiliencia, que se puede definir como mantener la capacidad productiva del animal frente a la infección. Existen numerosos informes que indican las razas locales como un importante reservorio de resistencia genética natural a las enfermedades como un proceso de adaptabilidad al medio ambiente. Refiriéndose a las principales enfermedades de los animales domésticos (tuberculosis, brucelosis, fiebre aftosa, etc.), numerosos genes han sido identificados y estudiados como biomarcadores de resistencia y tolerancia como los genes BOLA, CD, NOD y SLC11A1 entre los más importantes. Aunque el factor limitante para que los programas de reproducción incluyan resistencia a enfermedades genéticas es la necesidad de cuantificar los fenotipos de resistencia. Esto puede ser costoso y logísticamente difícil, y es una barrera significativa para la selección de la resistencia a las enfermedades. Por esta razón, las características de resistencia a la enfermedad son un objetivo atractivo para los estudios genómicos y generalmente son objeto de estos estudios.

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Association between SLC11A1 (NRAMP1) polymorphisms and susceptibility to tuberculosis in Chinese Holstein cattle

Cited by 24 publications

References 24 publications

Analysis of Genetic Variation in the Bovine SLC11A1 Gene, Its Influence on the Expression of NRAMP1 and Potential Association With Resistance to Bovine Tuberculosis

Analysis of Genetic Variation in the Bovine SLC11A1 Gene, Its Influence on the Expression of NRAMP1 and Potential Association With Resistance to Bovine Tuberculosis

How Pathogens Feel and Overcome Magnesium Limitation When in Host Tissues

Resistencia y resiliencia a las enfermedades en las razas de rumiantes locales: un enfoque en América del Sur

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