Association between SNPs at IL-17A and IL-17F and susceptibility to accelerated silicosis

Abstract

The association between single nucleotide polymorphisms (SNPs) in the interleukin (IL)-17 gene and silicosis has been evaluated in different populations. The aim of the present study was to analyze the association between SNPs at IL-17A (-832A/G) and IL-17F (+7488A/G) and susceptibility to accelerated silicosis in the Iranian Kurdish population. We studied 48 patients with accelerated silicosis and 62 controls. Genomic DNA was isolated using the "salting out" method. PCR-RFLP was performed for all SNPs typing. The frequencies of A/A, A/G, and G/G genotypes at IL-17A (-832A/G) were 4 (8.33%), 23 (47.92%), and 21 (43.75%) in patients and 5 (8.06%), 35 (56.45%), and 22 (35.48%) in controls, respectively. The frequencies of A and G alleles at IL-17 (-832A/G) were 31 (32.29%) and 65 (67.71%) in patients, and 45 (36.29%) and 79 (63.71%) in the controls, respectively. The frequencies of A/A, A/G, and G/G genotypes at IL-17F (+7488A/G) were 1 (2.08%), 47 (97.92%), and 0 (0%) in patients, and 11 (17.74%), 51 (82.26%), and 0 (0%) in the controls, respectively. The frequencies of A and G alleles at IL-17F (+7488A/G) were 49 (51.04%) and 47 (48.96%) in patients, and 73 (58.87%) and 51 (41.13%) in the controls, respectively. IL-17F (+7488A/G) genotype was more frequent among the cases compared with controls (97.92% vs. 82.26%). The frequency of the IL-17F (+7488A/G) genotype was significantly greater in patients with accelerated silicosis (odds ratio = 10.13 95%; confidence interval = 1.2-81.5; p = 0.008). The IL-17F (+7488A/G) genotype revealed a significantly increased risk of accelerated silicosis ( p < 0.05). The IL-17F (+7488 G) allele was associated with an increased risk of accelerated silicosis, but in the case of the IL-17A (-832A/G) polymorphism, a significant association was not observed.

Evaluation of Oxidative Stress Parameters and Genotoxic Effects in Patients With Work-Related Asthma and Silicosis

“…In our study, it was shown that serum 8-OHdG levels increased approximately 3 times in the exposure group and 2 times in the silicosis group compared with the control group. Hassani et al 44 demonstrated the importance of IL-17 in the development of lung fibrosis secondary to silica exposure. Recently, studies have shown that the IL-23/IL-17 axis has a significant impact on lung inflammation and pulmonary fibrosis 45 .…”

Silica‐associated lung disease: An old‐world exposure in modern industries

“…All had died by the end of the 3-year follow-up. 56 Thirty-two men screened in a small agate mill in Guangzhou, China, found 15 cases of accelerated silicosis (prevalence of 47%) 27 3 ‡ 40-52 -2 (67%) Iran (2000-2007) 28 23 § 29 (13.7) 2 (0.6) 10 (43%) Italy (2004-2008) 29 1 ¶ 54 4 1 UK (2015) 30 6 ‡ 33.8 (5.9) 13 (5.3) 0 Iran (2017) 31 48 41 46 § 33 (29-37) 11 (9-17) 1 (2%) USA (2014) 42 1 ¶ 37 10 0 USA (1999-2003) 43 35 § --35 (100%) Italy (2015) 44 3 ‡ 48 (3.5) 13 (6) 0 Israel (2016) 45 47 19 § 47 (13.33) 11 (3.58) -Belgium (2018) 48 2 ‡ -<10 0 Australia (2018) 49 7 § 44 (26-61) 7 (4-10) 1 LTx (1%) Sandblaster Spain (2015) 50 1 ¶ 27 5 1 Czech Republic (2015) 51 1 ¶ 38 3.2 1 Turkey (2018) 52 1 ¶ 28 2 0 USA (2018) † † 53 101 § ---Stone crusher India (2015) 54 1 2 8 1 0 India (2015) 55 1 3 5 2 0 Jewellery and semi-precious stone workers India (2015) 56 19 -3.4 (1.7) 12 (63%) Brazil (2017) 57 57 32 12.5 6 (11%) China (2018) 34 15 (47% prevalence) 29 (4.9) 3.5 (1.7)…”

Evaluation of interleukin-17 receptor (IL-17RA) gene expression in PBMCs of patients with premature coronary artery disease

“…Numerous infl ammatory cytokines contribute in every part of atherosclerosis (17)(18)(19)(20)(21)(22)(23)(24)(25). In this regard, the roles of chemoattractants including chemokine 2 (CCL2) and CCL5, which promote the recruitment of monocytes (18), as well as secretion of pro-infl ammatory cytokines: IL-1 β, IL-6, and TNF-α in NF-κB signaling in infl ammation (19) have been identifi ed.…”