Association between the Phe206Leu polymorphism of L-selectin and brucellosis

Abstract

Brucellosis remains a major zoonosis worldwide; therefore, better understanding of its immunology is a priority for the development of new therapeutic and vaccination strategies. Genetic factors appear to have an important role in the pathogenesis of infectious diseases such as brucellosis. Adhesion molecules, such as members of the selectin family, participate in the interaction between leukocytes and the endothelium, as well as in inflammatory cell recruitment. The impact of L-selectin polymorphisms on brucellosis has not so far been investigated. The aim of this study was to assess an L-selectin Phe206Leu (F206L) polymorphism in patients with active brucellosis, and to analyse its possible relationship with disease progression. A case-control association study was carried out on 619 subjects, including 374 patients with brucellosis and 245 age-and sex-matched healthy controls. Genomic DNA was isolated, and amplification of L-selectin genomic regions was performed by PCR incorporating sequence-specific primers (PCR-SSP) to distinguish the genotypes. The frequencies of the F206L polymorphism were studied. A significant difference in F206L polymorphism was found between patients with brucellosis and controls. The 206Leu allele was more frequent in patients than in healthy individuals (36?6 versus 28 %, P=0?003). In addition, there was an association between the presence of the 206Leu allele and a relapse of brucellosis (odds ratio 6?53, 95 % confidence interval 1?5-28?8, P=0?005). The higher frequency of L-selectin genotypes in patients with brucellosis than in control individuals, as well as the association between the 206Leu allele and the occurrence of brucellosis relapse, suggest that the F206L polymorphism could make individuals more vulnerable to brucellosis.

E‐selectin and L‐selectin polymorphisms in patients with periodontitis

“…Because L‐selectin also has a role in attracting leukocytes to inflammatory sites (28), we analyzed the L‐selectin Phe206Leu polymorphism in patients with periodontitis. Although recent studies have addressed the significant association between the Phe206Leu polymorphism of L‐selectin with certain disorders such as immunoglobulin A nephropathy (29) and brucellosis (22), we were not able to detect any statistically significant association of this polymorphism with periodontitis. This finding indicates that different genes appear to be related to host susceptibility for periodontitis.…”

“…The polymorphisms investigated in the present study have been previously associated with diseases/conditions such as coronary artery disease (16,17), atherosclerosis (18,19), high blood pressure (20), systemic lupus erythromatosus (21) and brucellosis (22). As no study has yet been undertaken on the possible association between the E‐selectin Ser128Arg (A561C) and L‐selectin Phe206Leu (T668C) polymorphisms and periodontal disease, we evaluated their contribution in the susceptibility to periodontitis.…”

Soluble l‐Selectin as an Independent Biomarker of Bronchial Asthma

“…However, the roles of these two variables in asthma have not been determined. In addition, our previous studies in an Iranian population indicated that there is an association between inflammatory diseases (coronary artery diseases and brucellosis) and F206L polymorphism of l ‐selectin . The present study was conducted to determine the association of plasma levels of s l ‐selectin and F206L polymorphism of l ‐selectin with asthma in a population from Hamadan province of Iran.…”

Polymorphism in ICAM-1, PECAM-1, E-selectin, and L-selectin genes in Tunisian patients with inflammatory bowel disease

“…In Chinese patients with coronary heart disease, the frequency of the L-selectin gene 213P allele was significantly higher than that in the control group [60]. The allelic frequency of 206L allele was also found significantly increased among Iranian patients with brucellosis than in healthy individuals [41]. Among Asian Indian pediatric patients with celiac disease [39], the allelic frequency of L-selectin 206 L was found at an increased frequency, although insignificant, in celiac disease patients as compared with healthy controls.…”

“…A polymorphism in the gene encoding L-selectin has been identified in exon 3, which encodes for the epidermal growth factor (EGF) domain. A phenylalanine to leucine mutation at position 206 and its association with celiac disease [39], coronary heart disease [40], and brucellosis [41] has been reported.…”