Association of mitochondrial function with feed efficiency in rainbow trout: Diets and family effects

Eya, Jonathan C.; Ashame, Martha F.; Pomeroy, Charles F.

doi:10.1016/j.aquaculture.2011.08.037

Cited by 33 publications

(23 citation statements)

References 49 publications

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“…The two enzymes play important roles for energy metabolism; therefore, abnormal activities of these enzymes could imply disorders in energy metabolism. Moreover, there is also a relationship between growth characteristics and mitochondrial gene expression, as a down-regulation of some mitochondrial genes was observed in slow-growing Rainbow Trout Oncorhynchus mykiss (Eya et al 2011). In the present study, the expression of ND1 and CYTB was downregulated.…”

Section: Variablementioning

confidence: 99%

“…In the present study, the expression of ND1 and CYTB was downregulated. According to the literature, reduced expression of ND1 suggests that the assembly of the entire complex I and the catalytic activity of this complex are impaired (Eya et al 2011). In addition, the lower activities of complex I could cause oxidative damage to the respiratory chain proteins due to the increased generation of reactive oxygen species (ROS; Bottje et al 2002).…”

Section: Variablementioning

confidence: 99%

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Association of Mitochondrial Dysfunction with Oxidative Stress and Immune Suppression in Blunt Snout Bream Megalobrama amblycephala Fed a High‐Fat Diet

Liu

et al. 2014

J Aqua Anim Hlth

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High-fat diets may have favorable effects on growth, partly based on protein sparing, but high-fat diets often lead to fatty liver (excessive fat deposition in the liver), which may be deleterious to fish growth and health. The goal of this study was therefore to investigate possible adverse effects and how they develop. Juvenile Blunt Snout Bream Megalobrama amblycephala (initial weight ± SE = 17.70 ± 0.10 g) were fed two diets (5% fat [control] or 15% fat). After 8 weeks, fish that were fed the 15% fat diet showed a high rate of mortality and poor growth. The histological results clearly showed that the high fat intake resulted in fat and glycogen accumulation and structural alterations of the hepatocytes, mitochondria, and nuclei. In the high-fat group, impairments of the mitochondria included mitochondrial swelling and the loss of cristae and matrix. Fish that were given the 15% fat diet exhibited low succinate dehydrogenase and Na(+),K(+)-ATPase activities and increased cytochrome-c release from the mitochondria. Expression of genes for complex I and III subunits of the mitochondrial respiratory chain were down-regulated in fish that received the high-fat diet. Increases in malondialdehyde level and the ratio of oxidized glutathione to reduced glutathione suggested oxidative stress in the livers of fish from the high-fat diet group. Moreover, the lower leukocyte count, lysozyme and alternative complement activities, and globulin level in fish that received the high-fat diet indicated suppressive immune responses. Overall, the intake of excessive fat impaired mitochondrial bioenergetics and physiological functions. The dysfunction of the mitochondria subsequently mediated oxidative stress and hepatocyte apoptosis, which in turn led to the reduced efficacy of the immune system.

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Section: Variablementioning

confidence: 99%

Section: Variablementioning

confidence: 99%

Association of Mitochondrial Dysfunction with Oxidative Stress and Immune Suppression in Blunt Snout Bream Megalobrama amblycephala Fed a High‐Fat Diet

Liu

et al. 2014

J Aqua Anim Hlth

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“…through influences on body size; Kaplan & Salthe 1979;Scott & Fore 1995) and thus impact fitness. Furthermore, variation in feeding performance has been linked to mitotype in other vertebrates (Bottje & Carstens 2009;Eya et al 2011), suggesting that this is a relevant aspect of organismal performance to evaluate in relation to mtDNA introgression.…”

Section: Hypothesismentioning

confidence: 99%

Individual performance in relation to cytonuclear discordance in a northern contact zone between long‐toed salamander (Ambystoma macrodactylum) lineages

2014

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Cytonuclear discordance in contact zones between related lineages is common, with mitochondrial clines often being displaced from clines in nuclear allele frequency. Proposed explanations for such a pattern include adaptive introgression of mtDNA or a neutral wake of mtDNA being left behind following hybrid zone movement. However, studies investigating these hypotheses are rare. Our previous survey of genetic variation in the long-toed salamander (Ambystoma macrodactylum) highlighted a potential case of cytonuclear discordance between two lineages in western Canada. Here, we use additional markers and samples to clarify the extent of this discordance. We simultaneously assess the feeding performance of individuals in a common environment to test for an association between mitotype and individual performance. The genetic results confirm a general pattern of cytonuclear discordance in the focal region. However, we also observed more limited introgression of a diagnostic nuclear marker. Intriguingly, although there were differences in individual performance associated with the transition between mitotypes, these differences were not fully explained by mitotype. Instead, the lowest performance was observed in individuals demonstrating the greatest mismatch between mtDNA and all nuclear markers, suggesting the potential for cytonuclear incompatibilities to be acting. These results highlight the complexity of understanding the causes and consequences of mtDNA introgression and cytonuclear discordance in contact zones.

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“…For instance, several studies have characterized responses of mitochondria to excessive dietary lipid level in terrestrial animals (De Schrijver & Privett, 1984;Slim et al, 1996;Korshunov et al, 1998;Skulachev, 1998;Sreekumar et al, 2002). Moreover, it has been demonstrated that high-feed efficiency (FE) is associated with increased mitochondrial function in terrestrial animals (Bottje & Carstens, 2009) as well as in some fish species (Eya et al, 2010(Eya et al, , 2011(Eya et al, , 2012. In these studies conducted on fishes, it was found that dietary fat and protein have significant effect on the mitochondrial enzymatic complexes I-V and the expression levels of genes encoding these complexes (Eya et al, 2010(Eya et al, , 2011(Eya et al, , 2012.…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary lipid levels on mitochondrial gene expression in low and high‐feed efficient families of rainbow trout Oncorhynchus mykiss

Eya

Yossa

Ashame

et al. 2014

Journal of Fish Biology

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A 2 × 3 factorial study was conducted to evaluate the effects of dietary lipid level on mitochondrial gene expression in mixed sex rainbow trout Oncorhynchus mykiss. Practical diets with a fixed crude protein content of 42%, formulated to contain 10% (42/10), 20% (42/20) and 30% (42/30) dietary lipid, were fed to triplicate groups of either low-feed efficient (F129; mean ± s.d. = 105.67 ± 3.04 g initial average mass) or high-feed efficient (F134; mean ± s.d. = 97.86 ± 4.02 g) families of fish, to apparent satiety, twice per day, for 108 days. At the end of the experiment, diets 42/20 and 42/30 led to similar fish condition factors, which were higher than that observed with diet 42/10 (P < 0.05). F134 fish fed diet 42/10 showed the highest hepato-somatic index, while there was no significant difference among all the other treatments (P < 0.05). When the group of F134 fish fed diet 42/10 was used as the calibrator for gene expression analysis, the five genes selected for their involvement in lipid metabolism (complex I-nd1, complex III-cytb, complex IV-cox1, complex IV-cox2 and complex V-atp6) were up-regulated in the muscle and down-regulated in both the liver and the intestine. There was a significant family × diet interaction regarding nd1, cox2 and atp6 in the liver; nd1, cytb, cox1, cox2 and atp6 in the intestine, and nd1, cytb, cox1, cox2 and atp6 in the muscle (P < 0.05). The overall results of this study constitute basic information for the understanding of molecular mechanisms of lipid metabolism at the mitochondrial level in fishes.

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Association of mitochondrial function with feed efficiency in rainbow trout: Diets and family effects

Cited by 33 publications

References 49 publications

Association of Mitochondrial Dysfunction with Oxidative Stress and Immune Suppression in Blunt Snout Bream Megalobrama amblycephala Fed a High‐Fat Diet

Association of Mitochondrial Dysfunction with Oxidative Stress and Immune Suppression in Blunt Snout Bream Megalobrama amblycephala Fed a High‐Fat Diet

Individual performance in relation to cytonuclear discordance in a northern contact zone between long‐toed salamander (Ambystoma macrodactylum) lineages

Effects of dietary lipid levels on mitochondrial gene expression in low and high‐feed efficient families of rainbow trout Oncorhynchus mykiss

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