Association of polymorphic variants in MSTN, PRL, and DRD2 genes with intensity of young animal growth in Pushkin breed chickens

Митрофанова, О.В.; Дементьева, Н. В.; Krutikova, Anna; Yurchenko, O. P.; Vakhrameev, Anatoly B.; Terletskiy, V. P.

doi:10.3103/s0095452717030082

Cited by 12 publications

(8 citation statements)

References 14 publications

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“…Tools for the detection of SNP are presented in Table 1. Polymerase chain reaction was performed according to the previously described procedure (Mitrofanova et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

Efficiency of using SNP markers in the <i>MSTN</i> gene in the selection of the Pushkin breed chickens

Дементьева¹,

Vakhrameev²,

Larkina³

et al. 2020

Vestn. VOGiS

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In the poultry industry, indicators reflecting the growth rate of young stock and the exterior characteristics of chickens are important benchmarks for breeding. Traditional selection based on phenotypic evaluation is characterized by low efficiency with a low character inheritance ratio and is difficult to apply in small groups of animals and birds bred in bioresource collections. The use of molecular genetic markers associated with economically important traits makes it possible to carry out early selection of birds. This entails an increase in the profitability of the poultry industry. Recently, single nucleotide polymorphisms (SNPs) have served as convenient markers for selection purposes. For five generations (P1–P5), an experimental selection of hens of the Pushkin breed was carried out for live weight. It was based on selection for single nucleotide polymorphism rs313744840 in the MSTN gene. As a result, a significant increase in the frequency of allele A in this gene, from 0.11 to 0.50, took place. The association of SNP markers with meat qualities in the experimental group led to changes in the exterior profile of an adult bird at 330 days of age. The individuals with the AA and AG genotypes had the greatest live weight and longest body. As a result of selection, the bird on average became larger due to an increase in the number of heterozygous individuals with long bodies and large chest girths. The depth of the chest and the width of the pelvis increased due to an increase in the frequency of allele A in the experimental population. A tendency towards an increase in these indicators with the substitution of G with A in the genotype was found. Saturation of the population with desirable alleles led to an increase in the average live weight of the chickens. Analysis of the exterior parameters of adult birds showed that this growth is achieved by increasing the depth and volume of the bird body, and not by increasing the length of the limbs. Thus, marker selection carried out for five generations in the experimental population of Pushkin breed chickens to increase body weight has reliably (p < 0.001) changed the exterior profile of adult birds.

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“…Tools for the detection of SNP are presented in Table 1. Polymerase chain reaction was performed according to the previously described procedure (Mitrofanova et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

Efficiency of using SNP markers in the <i>MSTN</i> gene in the selection of the Pushkin breed chickens

Дементьева¹,

Vakhrameev²,

Larkina³

et al. 2020

Vestn. VOGiS

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“…Ранее нами уже были выявлены взаи-мосвязи между живой массой кур и заме-ной rs313744840 в гене миостатина [6,8]. Животные, несущие аллель А по этой заме-не, обладали повышенной скоростью роста.…”

Section: таблицаunclassified

“…Сейчас особое внимание уделяется обнаружению одно-нуклеотидных замен (SnP) в смысловых участках миостатинового гена, для того, чтобы выявить их ассоциации с признаками продуктивности [5,6].…”

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The Effect of Different Diets of Feeding on the Result of Associative Analysis of Polymorphism in the MSTN Gene and Growth of Live Weight in Chickens

Дементьева¹,

Митрофанова²,

Kudinov³

2018

IJAFR (МЖПФИ)

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Современная генетика кур тесно связана с изучением полиморфизма генов, влияющих на рост и раз-витие птицы. Ген миостатина является одним из генов, ответственных за формирование мышечной массы у высших позвоночных. Целью нашей работы было определить влияние типа кормления на скорость роста курочек пушкинской породы в зависимости от генотипа по замене rs313744840 в гене миостатина. Одна группа кур (n = 40) выращивалась на кормах с повышенным содержанием протеина и обменной энергии, другая (n = 64) на корме для молодняка несушек. Курочки с генотипом АА, выращиваемые на бройлерных кормах, начали достоверно (p < 0,05) отличаться от своих сверстниц с генотипом AG с возраста 28 дней. Это преимущество в росте сохранялось до конца периода кормления кормом с повышенным содержанием протеина и обменной энергии. Для курочек, выращенных на кормах для молодняка кур-несушек, тенден-ция к преимуществу по живой массе у гомозигот АА наблюдалась до 40-дневного возраста, но отмеченные различия не были достоверными. К возрасту 90 дней эта группа незначительно обогнала гетерозигот AG по живой массе, а от гомозигот GG практически не отличалась. Курочки с генотипом АА, выращенные на корме с повышенным содержанием протеина и обменной энергии, имели достоверно большую живую массу лишь в возрасте 34 дня и 40 дней. Использование корма с повышенным содержанием протеина и обменной энергии позволяет реализовать генетический потенциал птицы. Husbandry, Sankt-Petersburg, e-mail: mo1969@mail.ru Modern genetics of chickens is closely related to the study of polymorphism of genes affecting the growth and development of birds. the MStngene is coding myostatin protein responsible for the formation of muscle mass in higher vertebrates. the purpose of our studies was to determine the influence of the type of feeding on the growth rate of the Pushkin breed chickens, depending on the genotype of SnP rs313744840 in the myostatin gene. one group of chickens (n = 40) was grown on broiler feed fodder the other (n = 64) at the stern for laying hens. Chickens with the genotype AA, grown on broiler feed, began to significantly (p < 0.05) differ from their peers with the AG genotype from the age of 28 days. this advantage in growth persisted until the end of the feeding period with food with an increased protein content and exchange energy. For chickens grown on feed for young laying hens, a tendency to predominate over live weight in AA homozygotes was observed up to 40 days of age, but the differences noted were not reliable. By the age of 90 days, this group slightly outperformed the heterozygote AG by the live weight, and from the homozygotes of GG was practically the same. Chickens with the AA genotype, grown on the stern with increased protein and metabolic energy, had a significantly large live weight only at the age of 34 days and 40 days. the use of feed with increased protein content and exchange energy makes it possible to realize the genetic potential of the bird. Ключевые слова: куры, пушкинская порода, живая масса, миостатин, ПЦР-ПДРФ THE EFFECT...

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“…In poultry, including chickens, plumage color can serve as a genetic marker, useful for the identification of breeds, populations and breeding groups with their characteristic features (Moiseyeva et al, 2012;Mitrofanova et al, 2017). The molecular genetic mechanism of the appearance of a particular type of color is not yet fully understood, since several genes can affect the same trait.…”

Section: Introductionmentioning

confidence: 99%

Molecular-genetic bases of plumage coloring in chicken

Макарова¹,

Митрофанова²,

Vakhrameev³

et al. 2019

Vestn. VOGiS

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The color of plumage in birds is an important feature, often determining descent to a particular species or breed. It serves as a key factor in the interaction of birds with each other due to their well-developed visual perception of the surrounding world. In poultry including chickens, the color of the plumage can be treated as a genetic marker, useful for identifying breeds, populations and breeding groups with their specific traits. The origin of diverse color plumage is the result of two interrelated physical processes, chemical and optical, due to which pigment and structural colors in the color are formed. The pigment melanin, which is presented in two forms, eumelanin and pheomelanin, is widely spread in birds. The basis for the formation of melanin is the aromatic amino acid tyrosine. The process of melano-genesis involves many loci, part of the complex expression of plumage color genes. In birds, the solid black color locus encodes the melanocortin 1 receptor (MC1R), mutations in which lead to a change in receptor activation and form different variants of the E locus. Using the GWAS analysis, possible genes affecting the formation of color in chickens were detected. The biosynthesis and types of melanin are affected by the activity of the enzyme tyrosine, and mutations in the tyrosinase gene (TYR) cause albinism in different species. The formation mechanism of brown, silver, gold, lavender and a number of other shades is determined by the influence on the work of the MC1R genes and TYR specific modifier genes. Thus, locus I currently associated with the PMEL17 gene inhibits the expression of eumelanin, and the MLPH gene affects tyrosinase function. Research on the mechanisms of formation of the secondary coloring of plumage in chickens is being actively conducted nowadays. The formation of a marble feather pattern is associated with the mutation of the endothelin B2 receptor (EDNRB2), in the coding part of the gene of which a polymorphism is found associated with the mo locus. The molecular base that causes the feather banding (locus B and autosomal recessive banding) is identified. Today, only some genes that determine the color of the plumage of chickens are studied and described. Different genes can produce similar plumage patterns, and different phenotypes can be determined by the polymorphism of a single gene. Using molecular methods, you can more accurately identify these differences. This overview shows the nature of melanin coloration in birds using the example of chickens of various breeds and also attempts to systematize knowledge about the molecular-genetic mechanisms of the appearance of various types of coloration.

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Association of polymorphic variants in MSTN, PRL, and DRD2 genes with intensity of young animal growth in Pushkin breed chickens

Cited by 12 publications

References 14 publications

Efficiency of using SNP markers in the <i>MSTN</i> gene in the selection of the Pushkin breed chickens

Efficiency of using SNP markers in the <i>MSTN</i> gene in the selection of the Pushkin breed chickens

The Effect of Different Diets of Feeding on the Result of Associative Analysis of Polymorphism in the MSTN Gene and Growth of Live Weight in Chickens

Molecular-genetic bases of plumage coloring in chicken

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