Association of polymorphisms in the calpain I, calpain II and growth hormone genes with tenderness in bovine M. longissimus dorsi

Correlation between expression level of the bovine DNAJA1 gene and meat tenderness was recently found in Charolais longissimus thoracis muscle samples, suggesting that this gene could play an important role in meat tenderness. Here, we report the validation of polymorphisms within the bovine DNAJA1 gene, and the haplotype variability and extent of linkage disequilibrium in the three main French beef breeds (Blonde d'Aquitaine, Charolais, Limousin). Genotyping 18 putative SNPs revealed that 16 SNPs were polymorphic within the breeds tested. Two SNPs were removed from further analyses as one SNP had a low genotyping call rate, while the other SNP was not in Hardy-Weinberg equilibrium. The degree of heterozygosity observed for the remaining 14 SNPs varied between breeds, with Charolais being the breed with the highest genetic variation and Blonde d'Aquitaine the lowest. Linkage disequilibrium and haplotype structure of DNAJA1 were different between breeds. Eighteen different haplotypes, including three shared by all breeds, were discovered, and two to three tag SNPs (depending on the breed) are sufficient to capture all the genetic variability seen in these haplotypes. The results of this study will facilitate the design of optimal future association studies evaluating the role of the DNAJA1 gene in meat tenderness.

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“…The effects of genetic markers derived from these two genes have been confirmed in independent studies [14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 73%

Genetic Variability and Linkage Disequilibrium Patterns in the Bovine DNAJA1 Gene

et al. 2009

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“…The SF average of Piedmontese, Simmental, Angus, Hereford, Irish, Charolais and their crossbreeds with the CAPN1 316 CC genotype found lower than the other two genotypes (GC and GG) [8,9,13,33] . Brahman breeds have a similar structure also [3,22] .…”

Section: Discussionmentioning

confidence: 79%

Boz Irk Sığırın Et Gevrekliğini Belirlemede CAST ve CAPN1 Genlerindeki Kimi SNP’lerin Kullanımı

Kök¹,

Atalay²

2017

Kafkas Univ Vet Fak Derg

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The aim of this study is to investigate the relationship between some genotypic characteristics of purebred Turkish Grey Cattle (TGC) and beef tenderness characteristics. There is a linear relationship between meat tenderness and the increasing calcium activity after slaughtering. Calpastatin (CAST) is a neutral protease inhibitor of Calpain (CAPN1) in mammalian tissues. The three polymorphic places in the CAST and the CAPN1 genes in cattle (UoG-CAST, CAPN1 316 and 4751) are known as the markers of beef quality. The relationship between the tenderness traits of the longissimus dorsi (LD) and the 3 beef quality markers (3 SNPs) in pure TGC have been investigated and discussed. PCR-RFLP and ARMS-PCR methods were utilized to identify the genotypes. In order to determine the water holding capacity (WHC), cooking loss (CL) and the shear force (SF), samples extracted from LD were probed. The average and standard error SF of a two-year-old TCG bred in extensive conditions was 4.339±0.217 kg/cm 2 for the heifers and 4.689±0.569 kg/cm 2 for bulls. Both alleles of the UoG-CAST (C/G), the CAPN1 316 (C/G) and the CAPN1 4751(C/T) polymorphisms in the samples were observed. The average SF of 3.943±0.441 kg/cm 2 , 4.537±1.666 kg/cm 2 , and 3.869±0.721 kg/cm 2 were used for the CAST-CC, the CAPN1 4751-CC and the CAPN1 316-GC, respectively in order to cut the muscle fibers of the genotypes that have a positive effect on tenderness. No cattle of the CAPN1 316-CC genotype was identified among the samples. The average and standart error WHC, CL and SF values for the entire sample including heifers and bulls were found as 11.693±0.761%, 26.952±0.636%, and 4.483±0.252 kg/cm 2 , respectively. In conclusion, the presence of genetic variation in specific SNP markers of beef tenderness in purebred TGC can contribute to the process of raising TGC with more tender meat.

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“…Significant associations with shear force (an objective measure of tenderness) were identified for three SNPs at the mu-calpain locus (Page et al 2002. The 316 and 4751 polymorphisms in particular have been validated in a number of independent populations (Corva et al 2007, Costello et al 2007, Curi et al 2009, Gill et al 2009, Morris et al 2006). An SNP in the calpain 3 gene has also been patented for association with tenderness (Barendse 2007).…”

Section: Dna Markers For Tendernessmentioning

confidence: 98%

Genomics of Fruit Quality and Disorders

2012

OMICs Technologies

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Association of polymorphisms in the calpain I, calpain II and growth hormone genes with tenderness in bovine M. longissimus dorsi

Cited by 30 publications

References 37 publications

Genetic Variability and Linkage Disequilibrium Patterns in the Bovine DNAJA1 Gene

Genetic Variability and Linkage Disequilibrium Patterns in the Bovine DNAJA1 Gene

Boz Irk Sığırın Et Gevrekliğini Belirlemede CAST ve CAPN1 Genlerindeki Kimi SNP’lerin Kullanımı

Genomics of Fruit Quality and Disorders

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