Association of RUNX2 and TNFSF11 genes with production traits in a paternal broiler line

Grupioni, Natália Vinhal; Stafuzza, Nedenia Bonvino; Carvajal, Alejandra; Ibelli, A. M. G.; Peixoto, J. O.; Ledur, Mônica Corrêa; Munari, Danísio Prado

doi:10.4238/gmr16019443

Cited by 10 publications

(8 citation statements)

References 28 publications

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“…The knockout of this gene, also known as CBFA1, in mice results in skeletal abnormalities and Cbfa1-null mutant embryos are smaller and their weight is reduced, compared with normal littermates (Otto et al 1997). Interestingly this gene has also been associated with carcass-and growth-related traits in broilers (Grupioni et al 2017) and goats (Jiang et al 2019). It might therefore be possible that RUNX2 has been the target of selection.…”

Section: Discussionmentioning

confidence: 99%

Detection of selection signatures in Limousin cattle using whole‐genome resequencing

et al. 2020

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Summary Limousin, a renowned beef breed originating from central France, has been selectively bred over the last 100 years to improve economically important traits. We used whole‐genome sequencing data from 10 unrelated Limousin bull calves to detect polymorphisms and identify regions under selection. A total of 13 943 766 variants were identified. Moreover, 311 852 bi‐allelic SNPs and 92 229 indels located on autosomes were fixed for the alternative allele in all sequenced animals, including the previously reported missense deleterious F94L mutation in MSTN. We performed a whole‐genome screen to discover genomic regions with excess homozygosity, using the pooled heterozygosity score and identified 171 different candidate selective sweeps. In total, 68 candidate genes were found in only 57 of these regions, indicating that a large fraction of the genome under selection might lie in non‐coding regions and suggesting that a majority of adaptive mutations might be regulatory in nature. Many QTL were found within candidate selective sweep regions, including QTL associated with shear force or carcass weight. Among the putative selective sweeps, we located genes (MSTN, NCKAP5, RUNX2) that potentially contribute to important phenotypes in Limousin. Several candidate regions and genes under selection were also found in previous genome‐wide selection scans performed in Limousin. In addition, we were able to pinpoint candidate causative regulatory polymorphisms in GRIK3 and RUNX2 that might have been under selection. Our results will contribute to improved understanding of the mechanisms and targets of artificial selection and will facilitate the interpretation of GWASs performed in Limousin.

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Section: Discussionmentioning

confidence: 99%

Detection of selection signatures in Limousin cattle using whole‐genome resequencing

et al. 2020

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“…Our research group has characterized the genetic architecture for growth and body composition traits in an experimental population originated from a Brazilian broiler line (TT Reference Population). Candidate genes participating in the performance, organs, carcass traits ( Venturini et al, 2015 ), and bone integrity ( Fornari et al, 2014 ; Grupioni et al, 2017 ) were already discovered in this broiler population. Also, QTL associated with production traits, including carcass fat deposition ( Moreira et al, 2018 ), feed efficiency, and body weight ( Moreira et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

SAP30 Gene Is a Probable Regulator of Muscle Hypertrophy in Chickens

et al. 2021

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Animals with muscle hypertrophy phenotype are targeted by the broiler industry to increase the meat production and the quality of the final product. Studies characterizing the molecular machinery involved with these processes, such as quantitative trait loci studies, have been carried out identifying several candidate genes related to this trait; however, validation studies of these candidate genes in cell culture is scarce. The aim of this study was to evaluate SAP30 as a candidate gene for muscle development and to validate its function in cell culture in vitro. The SAP30 gene was downregulated in C2C12 muscle cell culture using siRNA technology to evaluate its impact on morphometric traits and gene expression by RNA-seq analysis. Modulation of SAP30 expression increased C2C12 myotube area, indicating a role in muscle hypertrophy. RNA-seq analysis identified several upregulated genes annotated in muscle development in treated cells (SAP30-knockdown), corroborating the role of SAP30 gene in muscle development regulation. Here, we provide experimental evidence of the involvement of SAP30 gene as a regulator of muscle cell hypertrophy.

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“…Currently, there are many studies in the world aimed at identifying genomic associations between individual SNPs and breeding traits in chickens (Fornari et al, 2014;Cruz et al, 2015;Grupioni et al, 2017). This abundance of identified links in the future can help in the mapping of polymorphisms deterrmining complex traits in poultry.…”

Section: Introductionmentioning

confidence: 99%

Efficiency of using SNP markers in the <i>MSTN</i> gene in the selection of the Pushkin breed chickens

Дементьева¹,

Vakhrameev²,

Larkina³

et al. 2020

Vestn. VOGiS

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In the poultry industry, indicators reflecting the growth rate of young stock and the exterior characteristics of chickens are important benchmarks for breeding. Traditional selection based on phenotypic evaluation is characterized by low efficiency with a low character inheritance ratio and is difficult to apply in small groups of animals and birds bred in bioresource collections. The use of molecular genetic markers associated with economically important traits makes it possible to carry out early selection of birds. This entails an increase in the profitability of the poultry industry. Recently, single nucleotide polymorphisms (SNPs) have served as convenient markers for selection purposes. For five generations (P1–P5), an experimental selection of hens of the Pushkin breed was carried out for live weight. It was based on selection for single nucleotide polymorphism rs313744840 in the MSTN gene. As a result, a significant increase in the frequency of allele A in this gene, from 0.11 to 0.50, took place. The association of SNP markers with meat qualities in the experimental group led to changes in the exterior profile of an adult bird at 330 days of age. The individuals with the AA and AG genotypes had the greatest live weight and longest body. As a result of selection, the bird on average became larger due to an increase in the number of heterozygous individuals with long bodies and large chest girths. The depth of the chest and the width of the pelvis increased due to an increase in the frequency of allele A in the experimental population. A tendency towards an increase in these indicators with the substitution of G with A in the genotype was found. Saturation of the population with desirable alleles led to an increase in the average live weight of the chickens. Analysis of the exterior parameters of adult birds showed that this growth is achieved by increasing the depth and volume of the bird body, and not by increasing the length of the limbs. Thus, marker selection carried out for five generations in the experimental population of Pushkin breed chickens to increase body weight has reliably (p < 0.001) changed the exterior profile of adult birds.

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Association of RUNX2 and TNFSF11 genes with production traits in a paternal broiler line

Cited by 10 publications

References 28 publications

Detection of selection signatures in Limousin cattle using whole‐genome resequencing

Detection of selection signatures in Limousin cattle using whole‐genome resequencing

SAP30 Gene Is a Probable Regulator of Muscle Hypertrophy in Chickens

Efficiency of using SNP markers in the <i>MSTN</i> gene in the selection of the Pushkin breed chickens

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