Association of single nucleotide polymorphism of chicken uncoupling protein gene with muscle and fatness traits

Liu, S.; Wang, S.Z.; Li, Z.H.; Li, H.

doi:10.1111/j.1439-0388.2007.00654.x

Cited by 21 publications

(17 citation statements)

References 22 publications

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“…Many researchers attribute the striking difference to genetic variations, for example, SNP (Liu et al, 2007;Leng et al, 2009;Chen et al, 2012). In the present study, our results showed that PPARγ promoter was differentially methylated in the lean and fat chicken lines and that PPARγ promoter methylation and PPARγ mRNA expression were negative correlated, suggesting DNA methylation variation may also attribute to the chicken fat trait difference in the divergently selected chicken lines.…”

Section: Disussionmentioning

confidence: 46%

“…The Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF) have been established by long-term divergent selection on abdominal fat percentage (AFP) and plasma very low density lipoprotein concentration since 1996 (Liu et al, 2007). The G0 generation of the NEAUHLF both derived from the commercial Arbour Acres grandsire line, which was then divided into 2 lines according to plasma very low density lipoprotein concentration at 7 wk of age.…”

Section: Introductionmentioning

confidence: 99%

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Epigenetic DNA methylation in the promoters of Peroxisome Proliferator-Activated Receptor γ in chicken lines divergently selected for fatness1

Sun

Gao

Qiao

et al. 2014

Journal of Animal Science

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Peroxisome proliferator-activated receptor γ is a master regulator of adipocyte differentiation and function. Expression of PPARγ in mammals is regulated by DNA methylation; however, it is currently unknown whether chicken PPARγ expression is regulated by DNA methylation. To enhance our understanding of molecular mechanisms underlying chicken adipose tissue development and adipogenesis, we investigated the promoter methylation status and gene expression of PPARγ gene in Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF). Deoxyribonucleic acid methylation was analyzed by bisulfite sequencing method, and mRNA expression was detected by real-time quantitative real time reverse-transcription polymerase chain reaction (RT-PCR). The analyzed region located from -1,175 to -301 bp upstream of the translation start codon ATG contains 6 CpG dinucleotides, which are located at positions -1,014, -796, -625, -548, -435, and -383 bp, respectively. The results revealed that the 3 CpGs at positions -548, -435, and -383 bp showed differential methylation between the lean and fat chicken lines, but the other 3 CpG sites at positions -1,014, -796, and -625 bp did not. PPARγ gene promoter methylation in both chicken lines decreased with age, and PPARγ promoter methylation levels were significantly higher in lean than fat broilers at 2 wk of age (79.9 to 64.5%; P < 0.0001), at 3 wk of age (66.7 to 58.3%; P < 0.0001), and at 7 wk of age (50.0 to 42.7%; P = 0.0004). Real-time quantitative RT-PCR analysis showed that, negatively correlated with DNA methylation (Pearson's r = -0.653, P = 0.0057), PPARγ expression was increased with age and significantly lower in lean than fat chicken lines at 2, 3, and 7 wk of age (P < 0.0001). In conclusion, our findings suggest that chicken PPARγ is regulated by DNA methylation during adipose tissue development.

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Section: Disussionmentioning

confidence: 46%

Section: Introductionmentioning

confidence: 99%

Epigenetic DNA methylation in the promoters of Peroxisome Proliferator-Activated Receptor γ in chicken lines divergently selected for fatness1

Sun

Gao

Qiao

et al. 2014

Journal of Animal Science

Self Cite

View full text Add to dashboard Cite

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“…All the birds were under the same feeding conditions. The information about NEAUHLF and feeding conditions were as previously described (Liu et al 2007).…”

Section: Materials and Methods Experimental Populations And Managementmentioning

confidence: 99%

Cloning, tissue expression and polymorphisms of chicken Krüppel‐like factor 7 gene

Zhang¹,

Wang²,

Zhang³

et al. 2013

Animal Science Journal

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Krüppel-like factor 7 (KLF7) has been extensively studied in mammalian species, but its role in birds is still unclear. In the current study, cloning and sequencing showed that the full-length coding region of chicken KLF7 (Gallus gallus KLF7, gKLF7) was 891 bp long, encoding 296 amino acids. In addition, real-time RT-PCR analysis showed that gKLF7 was broadly expressed in all 15 chicken tissues selected, and its expression was significantly different in spleen, proventriculus, abdominal fat, brain, leg muscle, gizzard and heart between fat and lean broilers at 7 weeks of age. Additionally, one novel single nucleotide polymorphism (SNP), XM_426569.3: c. A141G, was identified in the second exon of gKLF7. Association analysis showed that this locus was significantly associated with fatness traits in Arbor Acres broiler random population and the eighth generation of Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF) population (P < 0.05). These results suggest that gKLF7 might be a candidate gene for chicken fatness traits.

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“…Sherman et al (2008) observed significant association between bovine UCP3 gene mutations and feed efficiency traits (average daily gain, feed conversion ratio and growth efficiency). Recently, some statistically significant associations of the UCP3 gene variants with fatness traits were also found in chicken (Zhao et al, 2006;Liu et al, 2007). Searching for polymorphisms of the porcine UCP3 gene have been already carried out in several European and Chinese native breeds (Li et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Association of porcine UCP3 gene polymorphisms with fatness traits in a PietrainJinhua F2 population

Chen¹,

Zhao²,

Zhu³

et al. 2011

Afr. J. Biotechnol.

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Uncoupling protein 3 (UCP3) is a mitochondrial transmembrane carrier which uncouples oxidative phosphorylation and plays an important role in energy homeostasis and fatty acid transporting. In this study, two missense mutations (T221C and A448G) were detected in porcine UCP3 gene. Genotype frequencies were analyzed using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) in eight pig breeds (n = 384). The results revealed that Chinese native breeds had fixed genotype CC at T221C polymorphic loci. Allele G at the A448G loci has higher frequency than allele A in all pure breeds, except for Pietrain. Association analysis between variants and fatness traits was carried out in a Pietrain×Jinhua F2 population (n = 274). The results showed that two single nucleotide polymorphism (SNPs) were significantly associated with intramuscular fat content (p = 0.023 and p = 0.000, respectively). These results indicated that single nucleotide polymorphism of UCP3 gene is potentially associated with fatness traits in pig.

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Association of single nucleotide polymorphism of chicken uncoupling protein gene with muscle and fatness traits

Cited by 21 publications

References 22 publications

Epigenetic DNA methylation in the promoters of Peroxisome Proliferator-Activated Receptor γ in chicken lines divergently selected for fatness1

Epigenetic DNA methylation in the promoters of Peroxisome Proliferator-Activated Receptor γ in chicken lines divergently selected for fatness1

Cloning, tissue expression and polymorphisms of chicken Krüppel‐like factor 7 gene

Association of porcine UCP3 gene polymorphisms with fatness traits in a PietrainJinhua F2 population

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