Association of single nucleotide polymorphisms in miR- 499 and miR-196a with susceptibility to breast cancer

Abstract

Purpose: To investigate the relationship between miR-499 rs3746444 and miR-196a rs11614913 polymorphisms, and susceptibility to breast cancer in an Iranian population. Methods: This case-control study was performed on a population of 200 subjects comprising 100 breast cancer patients (case/observation group) and 100 healthy individuals (control group). Amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) was used to genotype these polymorphisms. P-values and odd ratios were determined, and p-values < 0.05 and odd ratios > 1 were considered statistically significant. Results: There were no significant differences between observation and control groups with respect to rs11614913 T/C polymorphism. The rs11614913 T allele was not identified as a risk factor for susceptibility to breast cancer (OR = 0.86, 95 % CI = 0.85 -1.3, p = 0.46). However, there were significant differences between observation and control groups with respect to rs3746444 T/C polymorphism. It was observed that cytosine-cytosine (CC) (OR = 4.5,, p = 0.06), and cytosine-thymine (CT) (OR = 1.9, 95% CI = 1-3.6, p = 0.04) genotypes had protective influence against susceptibility to breast cancer. Conclusion: These results indicate that CC and CT genotypes are associated with reduced risk of breast cancer. In particular, the presence of C allele is significantly associated with a low risk of breast cancer. These findings may provide useful information for prevention and early diagnosis of breast cancer. This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest

Association between MIR499A rs3746444 polymorphism and breast cancer susceptibility: a meta-analysis

“…A similar observation was documented in homozygous and allele models when data from Hu et al 24 was removed. In addition, the homozygous model was also heavily influenced by Qi et al 29 , while the allele model was heavily influenced by Doulah et al 31 , He et al 15 , Omrani et al 28 and Qi et al 29 Despite this, the direction and magnitude of the association were not affected by any of the studies, and the P values were still at the borderline of statistical significance. In silico analysis.…”

“…The reference list of these records were also hand-searched to identify additional studies. Ultimately, 12 records (which comprised 13 studies) were included in the meta-analysis 15,16,[24][25][26][27][28][29][30][31][32][33] . We also included preliminary data from our laboratory in the meta-analysis, making the total number of included studies 14.…”

Evidences from a Systematic Review and Meta-Analysis Unveil the Role of MiRNA Polymorphisms in the Predisposition to Female Neoplasms

“…The full texts of the remaining 79 records were evaluated and 10 articles were excluded for the following reasons: insufficient reported data ( n : 4) [16,17,18,19], studying other genes or polymorphisms ( n : 3) [20,21,22], not a case-control design ( n : 2) [23,24], and a duplicate study ( n : 1) [25]. Finally, a total of 69 articles remained [26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67,68,69,70,71,72,73,74,75,76,77,78,79,80,81,82,83,84,85<...>…”

“…Overall, 31 association studies, including 24 studies on BCa, six studies on GCa, and one study on both BCa and GCa, were included [29,31,32,33,35,38,43,44,46,47,50,52,55,57,60,62,63,65,67,68,70,73,77,78,80,81,82,85,87,92]. The article by Linhares et al [57] is composed of two studies on separate ethnicities.…”

“…Among these, there were studies that were carried out among Asians ( n : 23), Caucasians ( n : 5), Africans ( n : 1), South Americans ( n : 1), or non-Caucasian Brazilians ( n : 1). The genotype counts among the control group of nine studies deviated from HWE expectations [31,33,50,55,57,60,70,78,92]. A meta-analysis of the association between miR-196a2 rs11614913 and the risk of female neoplasms was carried out while using thirty-one studies, including 11034 cases and 12955 controls, and the results revealed significant associations (Table 2 and Figure 2: top-right panel, pooled ORG (95%CI): 0.91 (0.83–0.99), P : 0.03).…”