Association Study between T2DM and CAPN10 SNP-19 (rs3842570) Polymorphism in Navi Mumbai Population

Abstract

Genetic research has brought a lot of new knowledge in the area of genetic predisposition of type 2 diabetes mellitus (T2DM). It has been proposed that excessive insulin resistance and obesity are also responsible for the higher incidence of type 2 diabetes. Calpain-10 (CAPN10) is a member of a large family of intracellular proteases. The polymorphism at deletion/insertion SNP19 of this gene influences susceptibility to T2DM. The aim of the study was to determine whether calpain-10 (ins/del SNP19) polymorphism contributes significantly to susceptibility to T2DM in population of Navi Mumbai. The study included randomly selected 75 patients of which 33 had T2DM and 42 served as control subjects. Mean waist-to-hip ratio, HDL, LDL, VLDL, cholesterol, and triglyceride showed no difference whereas mean of age, FBS, and body mass index showed significant differences between the control and diabetes subjects. Genotyping of calpain-10 (ins/del SNP19) polymorphism was performed by polymerase chain reaction method. Among 75 participants, for allele-specific SNP19, genotype frequencies of allele1 (2R-32 bp), heterozygous allele (2R-3R 32 bp), and allele 2 (3R-32 bp) were 20 (26.6%), 36 (48%), and 19 (25.3%) observed, respectively. The results from the present study have indicated that CAPN10 (SNP19) shows no significant association with T2DM and more extensive studies on T2DM using candidate gene approach may provide better preventive measures and potential disease diagnostic tools.

Association of CAPN10 gene (rs3842570) polymorphism with the type 2 diabetes mellitus among the population of Noakhali region in Bangladesh: a case-control study

“…For example, strong associations between CAPN10 polymorphisms and T2DM have been found in Mexican Mestizos, south Indians, Javanese, Koreans, Egyptians, Tunisian Arabs, and Spanish population. Conversely, studies conducted in the Iraqi and Navi Mumbai populations of India did not reveal a robust correlation with T2DM [ 51 , 52 ]. In this genetic association study, we evaluated whether the CAPN10 SNP-19 (rs3842570) was associated with the risk of T2DM among the Noakhali region of Bangladeshi population.…”

“…The association between CAPN10 polymorphism and the risk of T2DM has been established in certain populations [16,19,25,30,31,33,34,35,48]. However, this relationship has not been consistently observed across all populations investigated [49][50][51][52]. For example, strong associations between CAPN10 polymorphisms and T2DM have been found in Mexican Mestizos, south Indians, Javanese, Koreans, Egyptians, Tunisian Arabs, and Spanish population.…”

Dysregulated miRNA-375, IL-17, TGF-β, and Microminerals Are Associated with Calpain-10 SNP 19 in Diabetic Patients: Correlation with Diabetic Nephropathy Stages

“…The Calpain SNP-19 (rs3842570) SNP is an insertion-deletion polymorphism (HGVS: NC_000002.11: g. 241534262_2 41534293 dup 32) SNP-19 is a two-allele insertion/deletion (indel) polymorphism. Indel-19 (simple tandem repeat insertion/deletion variant with either two or three copies of a 32 bp fragment) is believed to be associated with a high risk of T2DM and an increased BMI [12].…”