Associations between novel single nucleotide polymorphisms in the Bos taurus growth hormone gene and performance traits in Holstein-Friesian dairy cattle

Abstract

Growth hormone, produced in the anterior pituitary gland, stimulates the release of insulin-like growth factor-I from the liver and is of critical importance in the control of nutrient utilization and partitioning for lactogenesis, fertility, growth, and development in cattle. The aim of this study was to discover novel polymorphisms in the bovine growth hormone gene (GH1) and to quantify their association with performance using estimates of genetic merit on 848 Holstein-Friesian AI (artificial insemination) dairy sires. Associations with previously reported polymorphisms in the bovine GH1 gene were also undertaken. A total of 38 novel single nucleotide polymorphisms (SNP) were identified across a panel of 22 beef and dairy cattle by sequence analysis of the 5' promoter, intronic, exonic, and 3' regulatory regions, encompassing approximately 7 kb of the GH1 gene. Following multiple regression analysis on all SNP, associations were identified between 11 SNP (2 novel and 9 previously identified) and milk fat and protein yield, milk composition, somatic cell score, survival, body condition score, and body size. The G allele of a previously identified SNP in exon 5 at position 2141 of the GH1 sequence, resulting in a nonsynonymous substitution, was associated with decreased milk protein yield. The C allele of a novel SNP, GH32, was associated with inferior carcass conformation. In addition, the T allele of a previously characterized SNP, GH35, was associated with decreased survival. Both GH24 (novel) and GH35 were independently associated with somatic cell count, and 3 SNP, GH21, 2291, and GH35, were independently associated with body depth. Furthermore, 2 SNP, GH24 and GH63, were independently associated with carcass fat. Results of this study further demonstrate the multifaceted influences of GH1 on milk production, fertility, and growth-related traits in cattle.

Genome-wide associations for feed utilisation complex in primiparous Holstein–Friesian dairy cows from experimental research herds in four European countries

“…For example, the leptin gene on BTA4 (Van der Banos et al, 2008), LEPR on BTA3 Banos et al, 2008), SCD on BTA26 (Macciotta et al, 2008), PGF on BTA10 (Seidenspinner et al, 2011), SPP1 on BTA6 (Sheehy et al, 2009), GH on BTA19 (Mullen et al, 2010) and GHR on BTA20 (Banos et al, 2008;Waters et al, 2011). For all these candidate genes we looked at the groups of SNPs positioned surrounding these genes.…”

The effect of polymorphism in gene of insulin-like growth factor-I on the serum periparturient concentration in Holstein dairy cows

“…It seems that de-regulation of translation, via the 5'-UTR sequences in TT and CT genotypes, is responsible for a significant reduction in IGF-I expression and associated to poor reproductive performance in dairy herds. Most of the previous studies conducted on the IGF-I polymorphisms with the growth traits in beef cattle breed [10,13] or milk traits [20,21] . We demonstrated an association between the CC genotype and higher concentration of IGF-I during the 14 d before calving to 45 d postpartum in Holstein dairy cows; while the cow with TT genotype had the lowest level of IGF-I.…”

“…The following PCR conditions were applied to each assay; 50 mM KCl, 10 20 min of inactivation at 65 曟. The digested PCR products were analyzed by agarose gel 3% in 1 暳 TrisAcetic Acid-EDTA (TAE) buffer.…”

“…Several studies reported periparturient changes of IGF-I blood concentration in cattle [18,19] . It has been reported significant associations between single nucleotide polymorphisms (SNPs) in IGF-I and production traits in Holstein-Friesian cattle [20][21][22] . However, the effect of IGF-I genotypes on its Objective: To investigate the relationship between polymorphism within the 5'-untranslated region (5'-UTR) of IGF-I gene and its periparturient concentration in Iranian Holstein dairy cows.…”

“…Finally, the genomic DNAs were eluted in a volume of 100 毺L supplied elution buffer. The extracted DNA was diluted to a working concentration of [10][11][12][13][14][15][16][17][18][19][20] ng/毺L, and 2.4 毺L of it was used as a template in PCR.…”