Associations between Streptococcus uberis strains from the animal environment and clinical bovine mastitis cases

Wente, Nicole; Klocke, Doris; Paduch, Jan-Hendrik; Zhang, Yanchao; Seeth, Martin tho; Zoche-Golob, Veit; Reinecke, F.; Möhr, E.; Krömker, Volker

doi:10.3168/jds.2019-16669

Cited by 39 publications

(41 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Resistance to β-lactam antibiotics was present, with 11 (15.5%) isolates resistant to penicillin, three (4.2%) to oxacillin, one to first-generation (cefazolin) and fourth-generation (cefquinome) cephalosporins, and two isolates resistant to third-generation (cefoperazone and ceftiofur). 1 Susceptible, 2 Intermediate, 3 Resistant, 4 Amoxicillin/clavulanic acid, 5 Ampicillin, 6 Cefazolin, 7 Cefoperazone, 8 Cefquinome, 9 Ceftiofur, 10 Enrofloxacin, 11 Erythromycin, 12 Florfenicol, 13 Lincomycin, 14 Oxacillin, 15 Penicillin, 16 Tetracycline, 17 Trimethoprim/sulfamethoxazole.…”

Section: Antimicrobial Profilingmentioning

confidence: 99%

“…Although its primary reservoir is the dairy farm environment [5][6][7], some Strep. uberis strains show a contagious transmission mode [8,9], deserving further investigation. Molecular genotyping contributes to understanding the modes of dissemination and can be useful in epidemiological studies of Strep.…”

Section: Introductionmentioning

confidence: 99%

“…uberis mastitis. The most commonly used subtyping methods are MLVA (Multiple Loci VNTR Analysis; VNTR, Variable Number of Tandem Repeats), MLST (MultiLocus Sequencing typing), PFGE (Pulsed Field Gel Electrophoresis), and RAPD-PCR (Random Amplified Polymorphic DNA-PCR) [8,[10][11][12]. Generally, Strep.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genotyping and Antimicrobial Susceptibility Profiling of Streptococcus uberis Isolated from a Clinical Bovine Mastitis Outbreak in a Dairy Farm

et al. 2021

View full text Add to dashboard Cite

Streptococcus uberis, an environmental pathogen responsible also for contagious transmission, has been increasingly implicated in clinical mastitis (CM) cases in Europe. We described a 4-month epidemiological investigation of Strep. uberis CM cases in an Italian dairy farm. We determined molecular characteristics and phenotypic antimicrobial resistance of 71 Strep. uberis isolates from dairy cows with CM. Genotypic variability was investigated via multiplex PCR of housekeeping and virulence genes, and by RAPD-PCR typing. Antimicrobial susceptibility was assessed for 14 antimicrobials by MIC assay. All the isolates carried the 11 genes investigated. At 90% similarity, two distinct clusters, grouping 69 of the 71 isolates, were detected in the dendrogram derived from the primer ERIC1. The predominant cluster I could be separated into two subclusters, containing 38 and 14 isolates, respectively. Strep. uberis strains belonging to the same RAPD pattern differed in their resistance profiles. Most (97.2%) of them were resistant to at least one of the drugs tested, but only 25.4% showed a multidrug resistance phenotype. The highest resistance rate was observed for lincomycin (93%), followed by tetracycline (85.9%). This study confirmed a low prevalence of β-lactam resistance in Strep. uberis, with only one isolate showing resistance to six antimicrobial classes, including cephalosporins.

show abstract

Section: Antimicrobial Profilingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genotyping and Antimicrobial Susceptibility Profiling of Streptococcus uberis Isolated from a Clinical Bovine Mastitis Outbreak in a Dairy Farm

et al. 2021

View full text Add to dashboard Cite

show abstract

“…According to the authors' research for the recent 15 years, pathogenic streptococci and staphylococci have been most often detected from raw milk and udder secretions of patients with mastitis in cows. A special place, as noted (Hadzevych et al, 2019;Wente et al, 2019), in studies of mastitis have enterobacteria from the group of Escherichia coli, such as Escherichia, Citrobacter, Enterobacter, Klebsiella, Serratia, as well as corynebacteria and pseudomonads. The increase in the content of somatic cells in milk due to the growth in the number of leukocytes and the entry of microorganisms into the mammary alveoli signals that a cow has mastitis.…”

Section: Introductionmentioning

confidence: 99%

Impact of various milking equipment on incidence of mastitis in dairy herd

Palii¹,

Kovalchuk²,

Boyko³

2020

Ukr J Ecol

View full text Add to dashboard Cite

It was found that the lowest percentage of cows with mastitis was recorded in winter: 10.3% (P≤0.001) when milking with ‘De Laval’ equipment and 17.7% (P≤0.001) when milking with the ADM-8A unit. The highest incidence of mastitis in cows was observed in summer: 21.0% (P≤0.001) and 27.1% (P≤0.001), respectively, when milking cows at milking parlors ‘De Laval’ and ADM-8A. It was found that, when using milking equipment ADM-8A, adult cows are 2.1-1.7 times more prone to mastitis compared to cows of the 1st and 2nd calving. When milking cows with the ‘De Laval’ unit, the incidence of mastitis in adult cows is 4.3-1.1 times higher than in cows of the 1st and 2nd calving. Gentle milking mode on the ‘De Laval’ equipment allowed to increase the number of completely healthy animals to 74%, which is 9.6% more than when using the milking unit ADM-8A (64.4%), and to reduce the incidence of clinical mastitis in cows by 3.4 times. It has been established that in cows with disorders in the udder the content of somatic cells in the secretion of the udder significantly changes (with a high degree of correlation) in all periods of the functional state of the body. Thus, during subclinical mastitis r=l0.72 (P≤0.001) and udder irritation r=l0.58 (P≤0.05). At the beginning of lactation, subclinical mastitis is accompanied with significant changes in the activity of enzymes: muramidase r=l0.84 (P≤0.001), lactoperoxidase r=l0.65 (P≤0.01) and lactoferrin r=l0.66 (P≤0.01).

show abstract

“…A microbial factor is of particular importance in the occurrence of mastitis. In this case, microorganisms can be a direct cause of mastitis or complicate the developmental processes in the udder that occur as a result of exposure of the mammary gland to adverse environmental factors (Wente et al, 2019). The inflammatory process in the mammary gland leads to damage and destruction of milk-producing cells, resulting in a bad secretion.…”

Section: Introductionmentioning

confidence: 99%

Species composition of microbiota of cows udder and raw milk quality at mastitis

Palii¹,

Ulko²,

Bogomolov³

et al. 2020

Ukr J Ecol

View full text Add to dashboard Cite

To increase the productivity of cows and improve the nutritional and sanitary-technological qualities of milk is one of the most important tasks in the development of dairy farming in all countries of the world. The cause of degradation of these parameters is such a widespread disease of cows as mastitis. The social significance of mastitis is manifested in the fact that the causative agents of mastitis in cows can cause disease in humans. Since streptococci and staphylococci play a leading role in mastitis, they are the most common in milk. Studies have shown that udder disease in cows with mastitis at the beginning of lactation has a certain seasonal nature and its main peaks occur in March-May and September-November. During these months, from 2.5% to 4.1% of cows from the total number of livestock of the farm were found to have mastitis. Physiochemical features of milk from the cows with mastitis were an increase in the number of somatic cells to 1,500,000/cm3, increase of milk pH to 7.3, caused by the breakdown of milk proteins into ammonia, the increase in milk electrical conductivity to 5.92-7.54 mS/cm, associated with the entry of sodium and chlorine ions from the blood into the milk. In studies of cows with mastitis, 16 species of bacteria were isolated from the secretion of the udder. In monoculture, the microflora, that was found in 30.5% of cows, consisted of E. coli, St. epidermidis, C. freundi, Sh. Dysentery, St. aureus, St. hyicus spp. Chromogenes, Str. Agalactiae, St. lentus, and St. intermediate. In 69.5% of cows with mastitis, the microflora was found in various associations. The most common associations of bacteria were St. epidermidis + St. aureus + Str. agalactiae + Str. haemolyticus; E. coli + Str. agalactiae; Str. agalactiae + St. epidermidis; St. epidermidis + St. aureus + Str. agalactiae. We developed a method for diagnosing mastitis in cows, which was to diagnose the disease at early stage of inflammation of the mammary glands by studying the composition of the milk the examined lobe of the udder.

show abstract

Associations between Streptococcus uberis strains from the animal environment and clinical bovine mastitis cases

Cited by 39 publications

References 36 publications

Genotyping and Antimicrobial Susceptibility Profiling of Streptococcus uberis Isolated from a Clinical Bovine Mastitis Outbreak in a Dairy Farm

Genotyping and Antimicrobial Susceptibility Profiling of Streptococcus uberis Isolated from a Clinical Bovine Mastitis Outbreak in a Dairy Farm

Impact of various milking equipment on incidence of mastitis in dairy herd

Species composition of microbiota of cows udder and raw milk quality at mastitis

Contact Info

Product

Resources

About