Associations between the White Sea invertebrates and oxygen-evolving phototrophic microorganisms

Molecular Identification and Ultrastructural and Phylogenetic Studies of Cyanobacteria from Association with the White Sea HydroidDynamena pumila(L., 1758)

“…Cyanophycin granules were rare. Despite the fact that this strain was able to greatly accumulate phycocyanin [7], phycobilisomes on ultrathin sections were not detected due to the high density of the surrounding cytoplasm. From the combination of morphological and ultrastructural signs of cyanobacteria 2Dp86E had similarities with the representatives of the genus Leptolyngbya , which includes groups of filamentous cyanobacteria in LPP-group B that exhibit very thin trichomes (<3  μ m) [17, 18].…”

“…Cyanobacterial isolates 1Dp66E-1, 2Dp86E, and 10Dp66E have been previously purified from the samples of the hydroid Dynamena pumila (L., 1758) collected in June and August of 2006 in the Rugozero Bay of the Kandalaksha Gulf of the White Sea (66° 34′ N, 33° 08′ E) [7]. Cyanobacterial isolates 1Dp66E-1, 2Dp86E, and 10Dp66E have been grown on solid (1.5% agar) and liquid medium BG-11 [9] at 25°C and an illumination of 50  μ mol photons m −2  s −1 .…”

“…Symbioses and associations between cyanobacteria and marine animals of high latitudes are poorly studied. Recently we isolated the several cyanobacteria from the associations with White Sea hydroid Dynamena pumila (L., 1758) [7]. Purification of the symbiotic cyanobacteria from the associations with animals is rarely successful.…”

“…Purification of the symbiotic cyanobacteria from the associations with animals is rarely successful. Four cyanobacteria were isolated and purified into culture from native samples of hydroid D. pumila and were identified based on morphological characteristics as representatives of the I, II, and IV subsections of phylum BX Cyanobacteria [7]. …”

Revealing of Non-Cultivable Bacteria Associated with the Mycelium of Fungi in the Kerosene-Degrading Community Isolated from the Contaminated Jet Fuel

“…Morphological features of fungal mycelium were evaluated by scanning electron microscopy (SEM). The samples were fixed with 2%( v / v ) glutaraldehyde and dehydrated through graded ethanol series (including 100% ethanol) [ 47 ]. Then, they were transferred into absolute acetone, dried at the CO 2 critical point on a Dryer HCP-2 (Hitachi, Tokyo, Japan), sputtered with gold and palladium in an IB-3 Ion Coater (Eico, Hitachinaka, Japan) [ 47 ], and examined under a scanning electron microscope JSM-6380LA (JEOL, Akishima, Japan).…”