Astragalus polysaccharide modulates ER stress response in an OVA-LPS induced murine model of severe asthma

Lü, Yuan; Xing, Qiong-Qiong; Ding, Dou; Zhao, Xia

doi:10.1016/j.ijbiomac.2016.09.058

Cited by 31 publications

(12 citation statements)

References 48 publications

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“…We can nd that treatment effects of WYHY decoction is better than XQL decoction in the regulation of autophagy and improved airway in ammation. The reason may be that WYHY added Ginseng (Radix et Rhizoma) and Astragali (Radix) on the basis of XQL decoction.Many experimental studies have found that Ginseng and Astragali can regulating the autophagy related gene through PI3K-mTOR pathway [44][45][46][47][48][49], and Ginseng and Astragali can also alleviate various kinds of in ammation [50][51][52][53][54]. Therefore, according to the results of this experiment, it can be concluded that WYHY decoction can better regulate autophagy and alleviate airway in ammation of cold asthma rats by added Ginseng (Radix et Rhizoma) and Astragali (Radix).…”

Section: Discussionmentioning

confidence: 81%

Winyanghuayin Decoction and Xiaoqinglong Decoction Treatment Airway Inflammation of Cold Asthma Through PI3K-mTOR Signal Pathway

Chang

Zhang

Qing-xiang

et al. 2020

Preprint

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Abstract Background: Airway inflammation plays a pivotal role in cold asthma. Winyanghuayin decoction and Xiaoqinglong decoction can significantly improve airway inflammation. The aim of the present study was to explore the therapeutic effect and mechanism of Winyanghuayin decoction and Xiaoqinglong decoction on OVA-induced cold asthma in rat and compare the effects of Winyanghuayin decoction and Xiaoqinglong decoction for treatment of cold asthma ratsMethods: The cold asthma rat model was induced by OVA and ice water swim stress, treated with Winyanghuayin decoction and Xiaoqinglong decoction respectively. The pulmonary function of asthmatic rats was detected by MIR Spirolab; The pathological changes of lung tissue were observed by HE staining; The autophagy in the lung of rats were observed by transmission electron microscopy; The eosinophils, lymphocytes, macrophages and total cells in bronchoalveolar lavage fluid (BALF) were counted under light microscope. The expression of IL-10\IL-13\TNF-α and TGF-β1 in serum were measured by enzyme-linked immunosorbent assay (ELISA); The protein level of LC3-II\I, ATG, PI3K and mTOR expression in lung tissues were detected by Western blot; Real-time polymerase chain reaction (RT-PCR) detected the expression of autophagy gene ATG3\ATG5\ATG7 and ATG12.Results: Winyanghuayin decoction and Xiaoqinglong decoction can alleviate airway inflammation and improve airway hyper-responsiveness, significantly increased the level of autophagy in lung tissue and decreased the protein level of PI3K and mTOR in lung tissue of cold asthma rats. Winyanghuayin decoction have better regulation ability on autophagy and PI3K-mTOR signaling pathway.Conclusion: Winyanghuayin decoction and Xiaoqinglong decoction may alleviate the airway inflammation symptoms by enhancing autophagy in lung tissue dependent on the PI3K-mTOR signaling pathway. Winyanghuayin decoction was more effective than Xiaoqinglong decoction for treatment of cold asthma.

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Section: Discussionmentioning

confidence: 81%

Winyanghuayin Decoction and Xiaoqinglong Decoction Treatment Airway Inflammation of Cold Asthma Through PI3K-mTOR Signal Pathway

Chang

Zhang

Qing-xiang

et al. 2020

Preprint

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“…For example, avian pathogenic E. coli challenge activated TLR4 and NF-κB and stimulated the expression of ICAM-1, VCAM-1, TNF-α and IL-1β in 21-day old chicken ileum intestinal tissue (Zhong et al, 2014). APS inhibited the increase in the expression levels of TLR4 and NF-κB in 35-day old broilers jejunal mucosa (Liu et al, 2015) and the translocation of NF-κB in murine (Lu et al, 2016) with LPS-induced. Our findings showed that APS inhibited the increase induced by E. coli in both TLR4 and NF-κB mRNA expression in 7-day old chicken's intestine tissue, which coincided with the notion that APS inhibited E. coli induced increase of ICAM-1, VCAM-1, TNF-α, IL-1β and iNOS mRNA expression, and alleviated the decline in EGF and bFGF mRNA expression.…”

Section: Discussionmentioning

confidence: 99%

Suppresses of Astragalus Polysaccharide on E. coli-Induced Injured Intestinal Microvascular through TLR4-NF-κB Signal Pathways in Chickens

Wang

Huang

et al. 2019

Braz. J. Poult. Sci.

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To investigate the hypothesis that APS can attenuate E. coli-induced microvascular dysfunction in chicken intestine, 60 0-day old male chickens were divided into three groups with 5 replications of 4 chicks. Chicken in the APS group were treated with 15 mg APS daily while the others were given 0 mg APS for 6 days. Then all 7-day old chicken were injected intraperitoneally by E. coli, except for the chicken in the control group. After 4 h, all chicken's intestinal samples were collected to detect gene expressions involved in inflammatory factors and adhesion molecules. Results showed that APS attenuated the signs of edema and hemorrhage in 7-day old chicken intestinal mucosa which were induced by E. coli injection. Consistently, APS significantly reduced the increasing mRNA levels of inflammatory factors such as Tumor necrosis factor-a (TNF-a), interleukin (IL)-1β and inducible nitric oxide synthase (iNOS) (p<0.05), the same results were observed in vascular adhesion molecules such as E-selectin, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). Moreover, we observed that APS supplementation in water suppressed significantly (p<0.05) the decline of reparative factors such as epithelial growth factor (EGF) and basic fibroblast growth factor (bFGF) in E. coli injected group. Furthermore, supplementation with APS substantially blocked (p<0.05) the increase in Toll-like receptor-4 (TLR4) and Nuclear factor (NF)-κB mRNA abundance (p=0.087) induced by E. coli infection. This study indicated that microvascular injured chicken intestine induced by E. coli would be attenuated with feeding APS, and the mechanism of repairing were probably mediated through TLR4-NF-κB signal pathways.

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“…Recently, monomers of Chinese traditional herbs have attracted more and more attention because of their desirable pharmacological effects. Many monomers from Chinese medicine were valuable in the treatment of asthma, such as baicalin [ 11 ], salidroside [ 12 ], astragalus membranaceus [ 13 ], and luteolin [ 14 ]. Although most of these monomers were investigated in murine models, their therapeutic effects shown on asthma are promising.…”

Section: Discussionmentioning

confidence: 99%

Paeonol Ameliorates Ovalbumin‐Induced Asthma through the Inhibition of TLR4/NF‐κB and MAPK Signaling

Tang

Huang

Song

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Asthma is a chronic inflammatory disease of the airways, with complex signaling pathways involved in its pathogenesis. It was reported that paeonol attenuated airway inflammation of ovalbumin (OVA)-induced mice. Therefore, it is of importance to further investigate the underlying mechanism. BALB/c mice were challenged with OVA for the asthma model, which was validated by the changed levels of IL-4, IFN-γ, and IgE. The elevation of IL-4 and the decreasing of IFN-γ were significantly in middle (p<0.05) or high (p<0.01) paeonol dose groups compared with OVA group. MIP-1β in bronchoalveolar lavage fluid (BALF) also decreased significantly in middle and high paeonol group compared with OVA group (p<0.01), which is similar to the change of its mRNA in lung tissues. Moreover, the inflammatory cells infiltration and collagen deposition were attenuated by paeonol and montelukast sodium via histology examination. At last the immune blot of the protein extracted from lung tissues demonstrated that paeonol decreased the expression of TLR4 and the nuclear translocation of NF-κB, as well as the phosphorylation levels of P38 and ERK in asthma model. In conclusion, paeonol ameliorated OVA-induced asthma through the TLR4/NF-κB and mitogen-activated protein kinase (MAPK) signaling.

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Astragalus polysaccharide modulates ER stress response in an OVA-LPS induced murine model of severe asthma

Cited by 31 publications

References 48 publications

Winyanghuayin Decoction and Xiaoqinglong Decoction Treatment Airway Inflammation of Cold Asthma Through PI3K-mTOR Signal Pathway

Winyanghuayin Decoction and Xiaoqinglong Decoction Treatment Airway Inflammation of Cold Asthma Through PI3K-mTOR Signal Pathway

Suppresses of Astragalus Polysaccharide on E. coli-Induced Injured Intestinal Microvascular through TLR4-NF-κB Signal Pathways in Chickens

Paeonol Ameliorates Ovalbumin‐Induced Asthma through the Inhibition of TLR4/NF‐κB and MAPK Signaling

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