Asymmetric polymerase chain assay combined with propidium monoazide treatment and unmodified gold nanoparticles for colorimetric detection of viable emetic Bacillus cereus in milk

Li, Fan; Li, Fulai; Yang, Guotai; Aguilar, Zoraida P.; Lai, Weihua; Xu, Hengyi

doi:10.1016/j.snb.2017.08.154

Cited by 50 publications

(24 citation statements)

References 26 publications

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“…The assay has been demonstrated for sensitive detection of HIV DNA with the naked eye, showing great promise for use in the POC settings. More recently, Xu et al demonstrated that unmodified Au NPs could also be used for colorimetric detection of asymmetric PCR products by combining salt-induced nanoparticle aggregation assay [119]. In this design, PCR-produced single-stranded products protected Au NPs from aggregation in the presence of high salt concentration.…”

Section: Plasmonics-enhanced Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Plasmonic molecular assays: Recent advances and applications for mobile health

Wei

2018

Nano Res.

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Plasmonics-based biosensing assays have been extensively employed for biomedical applications. Significant advancements in use of plasmonic assays for the construction of point-of-care (POC) diagnostic methods have been made to provide effective and urgent health care of patients, especially in resourcelimited settings. This rapidly progressive research area, centered on the unique surface plasmon resonance (SPR) properties of metallic nanostructures with exceptional absorption and scattering abilities, has greatly facilitated the development of cost-effective, sensitive, and rapid strategies for disease diagnostics and improving patient healthcare in both developed and developing worlds. This review highlights the recent advances and applications of plasmonic technologies for highly sensitive protein and nucleic acid biomarker detection. In particular, we focus on the implementation and penetration of various plasmonic technologies in conventional molecular diagnostic assays, and discuss how such modification has resulted in simpler, faster, and more sensitive alternatives that are suited for point-of-use. Finally, integration of plasmonic molecular assays with various portable POC platforms for mobile health applications are highlighted.

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Section: Plasmonics-enhanced Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Plasmonic molecular assays: Recent advances and applications for mobile health

Wei

2018

Nano Res.

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“…Short shelf-life and easy contamination is mostly common in this product. Studies evaluating the detection of pathogens in spiked milk using biofunctionalized inorganic nanoparticles has been reported [68,76,91,95,100,101,106,117]. Investigation by Ma et al [63] on the colorimetric detection of S. typhi using aptamer modified-AuNPs had 96.4% recoveries similar to those obtained using the plate counting methods (104.3%).…”

Section: Application Of Functionalized Inorganic Nps For Pathogens Dementioning

confidence: 74%

“…Incorporation and combination of functionalized inorganic nanoparticles with molecular biological tools such as DNA extraction and PCR reagents as reported in some reviewed studies is gradually enhancing its application in rapid, real-time and pathogen detection [120]. Other works by Li et al [117] and Du et al [121] documented that inorganic nanoparticles could still produce some colorimetric signals without binding to any bio-receptor. This suggests that direct attachment to PCR products with some concentration of salt solution ensures the accuracy of PCR products by eliminating false-positive results.…”

Section: Advantages Of Inorganic Nanoparticles To Signal Amplificationmentioning

confidence: 99%

Functionalized Inorganic Nanoparticles for the Detection of Food and Waterborne Bacterial Pathogens

Daramola¹,

Torimiro²,

Fadare³

et al. 2020

NNR

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Infections acquired from ingesting contaminated food and water poses an adverse effect on public health and safety, thus affecting nations' economy. Technical approaches developed over years have contributed adequately to microbial detection in food and water, yet, unveiling spaces for more improvement on early and rapid detection of pathogens. This review highlights different strategy assessing bio-functionalized inorganic nanoparticles towards the detection of pathogens in food and water samples. Conjugates of several bio-receptors and inorganic nanoparticles showed rapid, real-time, repeatability, and appreciable limit of detection in targeted pathogens. A patent referenced in this study established the biocompatibility of bio-functionalized inorganic nanoparticles mechanism. Unique attributes exhibited by bio-functionalized inorganic nanoparticles showed potential and improvement of the existing bio-sensing pathogen detection methods. Each of the identified strategies described showed a promising pathway accommodating the development of simple, and even the fabrication of low-cost materials for easy detection of bacterial pathogens in food and water products.

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“…This type of assay can utilize the strong absorption and light scattering properties of GNPs that were described above. GNPs have extinction coefficients that are >1,000 times higher than organic dyes, and the colorimetric changes display high sensitivity (Niu et al, 2014) and can often be detected by the naked eye (Bui, Ahmed, & Abbas, 2015;Du et al, 2018;Kim et al, 2018;Li et al, 2018). In order to achieve specificity toward foodborne pathogens of interest, targeting moieties such as antibodies (Bui et al, 2015), oligonucleotides (Du et al, 2018), or aptamers (Kim et al, 2018;Ma, Song, Zhou, Xia, & Wang, 2017;Niu et al, 2014) can be conjugated to the surfaces of GNPs.…”

Section: Solution-based Colorimetric Assays Using Gnpsmentioning

confidence: 99%

“…Viable emetic Bacillus cereus was similarly visually detected in milk by Li et al (2018) using an unmodified GNP assay developed with propidium monoazide (PMA)-asymmetric polymerase chain reaction (as PCR). In this assay, the PMA serves to eliminate false positives given by non-viable bacteria present in tested samples as it prevents amplification of PMAintercalated DNA.…”

Section: Solution-based Colorimetric Assays Using Gnpsmentioning

confidence: 99%

Single and multiple detections of foodborne pathogens by gold nanoparticle assays

Pissuwan

Gazzana

Mongkolsuk

et al. 2019

WIREs Nanomed Nanobiotechnol

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A late detection of pathogenic microorganisms in food and drinking water has a high potential to cause adverse health impacts in those who have ingested the pathogens. For this reason there is intense interest in developing precise, rapid and sensitive assays that can detect multiple foodborne pathogens. Such assays would be valuable components in the campaign to minimize foodborne illness. Here, we discuss the emerging types of assays based on gold nanoparticles (GNPs) for rapidly diagnosing single or multiple foodborne pathogen infections. Colorimetric and lateral flow assays based on GNPs may be read by the human eye. Refractometric sensors based on a shift in the position of a plasmon resonance absorption peak can be read by the new generation of inexpensive optical spectrometers. Surface‐enhanced Raman spectroscopy and the quartz microbalance require slightly more sophisticated equipment but can be very sensitive. A wide range of electrochemical techniques are also under development. Given the range of options provided by GNPs, we confidently expect that some, or all, of these technologies will eventually enter routine use for detecting pathogens in food. This article is categorized under: Diagnostic Tools > Biosensing

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Asymmetric polymerase chain assay combined with propidium monoazide treatment and unmodified gold nanoparticles for colorimetric detection of viable emetic Bacillus cereus in milk

Cited by 50 publications

References 26 publications

Plasmonic molecular assays: Recent advances and applications for mobile health

Plasmonic molecular assays: Recent advances and applications for mobile health

Functionalized Inorganic Nanoparticles for the Detection of Food and Waterborne Bacterial Pathogens

Single and multiple detections of foodborne pathogens by gold nanoparticle assays

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