At RAV1 and At RAV2 overexpression in cotton increases fiber length differentially under drought stress and delays flowering

Characterization of transgenic cotton (Gossypium hirsutum L.) over-expressing Arabidopsis thaliana Related to ABA-insensitive3(ABI3)/Vivparous1 (AtRAV1) and AtABI5 transcription factors: improved water use efficiency through altered guard cell physiology

“…2A), 361 which is consistent with the pattern seen for well-watered plants after eight days ( Fig. 2A) and 362 previous molecular and physiological results that showed these transgenics have a "less stressed" 363 phenotype (Mittal et al 2014(Mittal et al , 2015. With the caveat that relative AtABI5 protein levels in 364 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 13 seeds (Fig.…”

“…With the caveat that relative AtABI5 protein levels in 364 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 13 seeds (Fig. 1) may not reflect similar levels in leaves, taken together with prior results for 365 phenotypic effects of these specific ABI5 events correlating with increases in leaf area, WUE, 366 root biomass, and fiber length (Mittal et al, 2014(Mittal et al, , 2015, the immunoblot and ABA 367 quantifictation results are consistent with the working hypothesis of increased ABA 368 sensitivity/homeostasis in ABI5-and RAV1 over-expressing lines. analyzed the relationship between pore size and guard cells (i.e., pore area / guard cell area).…”

“…This was done using a two-tailed Student's t-test, assuming equal 286 variance except for ABA quantifictation, where a one-tailed t-test was used on grounds prior 287 work (Mittal et al 2014(Mittal et al , 2015 showed the test genotypes were drought tolerant/ABA 288 hypersensitive. 289…”

“…This observation is consistent 301 with prior results from greenhouse and field that the AtABI5 1-1-1 event has more extreme 302 phenotypic effects than AtABI5 13-4-1 for longer fiber lengths, larger leaf area, greater root 303 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 biomass, and higher WUE (Mittal et al, 2014(Mittal et al, , 2015. LA lower, but still detectable (inset arrow), 304 that endogenous stress response markers Responsive to ABA18 (GhRAB18), GhRAV, 325…”

“…Previous evidence showed that AtRAV1-and AtABI5-over-expressing cotton had "less 336 stressed" and drought avoidance phenotypes under imposed drought (Mittal et al 2014(Mittal et al , 2015, 337 therefore we measured ABA levels in cotyledons in response to drought stress to independently 338 address the hypothesis that ABA signaling processes may be impacted in these lines. On day 339 seven under drought-stress, control Coker312 plants showed a 'wilty' phenotype and had ABA 340…”