Atlantic salmon (Salmo salar L.) serum vitellogenin neutralises infectivity of infectious pancreatic necrosis virus (IPNV)

García, José Ángel López; Munro, E S; Monte, Milena Mira; Fourrier, Mickael; Whitelaw, Jason; Smail, D. A.; Ellis, A.E.

doi:10.1016/j.fsi.2010.04.010

Cited by 74 publications

(41 citation statements)

References 18 publications

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“…Vitellogenin is synthesized in different tissues in different species, and eventually accumulates in the ovary, where it promotes ovarian development (Jeon et al, 2010). Vitellogenin was recently reported to play a role in the host immune system in fish (Garcia et al, 2010). In the present study, vitellogenin and the vitellogenin receptor were not found to be expressed in testes, whereas there was moderate expression in the ovary, suggesting that vitellogenin may be involved in ovarian development.…”

Section: Genes Related To Reproduction and Sex Determinationcontrasting

confidence: 43%

Comparative transcriptome analysis of testes and ovaries for the discovery of novel genes from Amur sturgeon (Acipenser schrenckii)

Jin¹,

Zhang²,

Dong³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Sturgeons (Acipenser schrenckii) are of high evolutionary, economic, and conservation value, and caviar isone of the most valuable animal food products in the world. The Illumina HiSeq2000 sequencing platform was used to construct testicular and ovarian transcriptomes to identify genes involved in reproduction and sex determination in A. schrenckii. A total of 122,381 and 114,527 unigenes were obtained in the testicular and ovarian transcriptomes, respectively, with average lengths of 748 and 697 bp. A total of 46,179 genes were matched to the nonredundant nr database. GO (31,266), KEGG (39,712), and COG analyses (20,126) were performed to identify potential genes and their functions. Twenty-six gene families involved in reproduction and sex determination were identified from the A. schrenckii testicular and ovarian transcriptomes based on functional annotation of non-redundant transcripts and comparisons with the published literature. Furthermore, 1309 unigenes showed significant differences between the testes and ovaries, including 782 genes that were up-regulated in the testes and 527 that were upregulated in the ovaries. Eleven genes were involved in reproduction and sex determination mechanisms. Furthermore, 19,065 simple sequence repeats (SSRs) were identified in the expressed sequence tagged dataset, and 190,863 and 193,258 single nucleotide polymorphisms (SNPs) were obtained from the testicular and ovarian transcriptomic databases, respectively. This study provides new sequence information about A. schrenckii, which will provide a basis for the further study of reproduction and sex determination mechanisms in Acipenser species. The potential SSR and SNP markers isolated from the transcriptome may shed light on the evolution and molecular ecology of Acipenser species.

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Section: Genes Related To Reproduction and Sex Determinationcontrasting

confidence: 43%

Comparative transcriptome analysis of testes and ovaries for the discovery of novel genes from Amur sturgeon (Acipenser schrenckii)

Jin¹,

Zhang²,

Dong³

et al. 2015

Genet. Mol. Res.

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“…Knowledge about this protein could, eventually, provide unique illustrations on how insect social organization can be achieved at the level of protein structure. Moreover, insights into the molecular properties of vitellogenins are of broader general interest, as alternative functions of this family of yolk precursor proteins are now surfacing in diverse taxa: including in the immune defense against viruses and bacteria in fish (Garcia et al, 2010;Li et al, 2008;Shi et al, 2006), and in aging in Caenorhabditis elegans (Nakamura et al, 1999). …”

Section: Discussionmentioning

confidence: 99%

Deconstructing honeybee vitellogenin: novel 40 kDa fragment assigned to its N terminus

Havukainen

Halskau

Skjærven

et al. 2011

Journal of Experimental Biology

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SUMMARYVitellogenin, an egg-yolk protein precursor common to oviparous animals, is found abundantly in honeybee workers -a caste of helpers that do not usually lay eggs. Instead, honeybee vitellogenin (180kDa) participates in processes other than reproduction: it influences hormone signaling, food-related behavior, immunity, stress resistance and longevity. The molecular basis of these functions is largely unknown. Here, we establish and compare the molecular properties of vitellogenin from honeybee hemolymph (blood) and abdominal fat body, two compartments that are linked to vitellogenin functions. Our results reveal a novel 40kDa vitellogenin fragment in abdominal fat body tissue, the main site for vitellogenin synthesis and storage. Using MALDI-TOF combined with MS/MS mass-spectroscopy, we assign the 40kDa fragment to the N terminus of vitellogenin, whereas a previously observed 150kDa fragment corresponded to the remainder of the protein. We show that both protein units are N glycosylated and phosphorylated. Focusing on the novel 40kDa fragment, we present a homology model based on the structure of lamprey lipovitellin that includes a conserved b-barrel-like shape, with a lipophilic cavity in the interior and two insect-specific loops that have not been described before. Our data indicate that the honeybee fat body vitellogenin experiences cleavage unlike hemolymph vitellogenin, a pattern that can suggest a tissue-specific role. Our experiments advance the molecular understanding of vitellogenin, of which the multiple physiological and behavioral effects in honeybees are well established. Supplementary material available online at

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“…Установлено, что овариальная жидкость содержит полный набор заменимых и незаменимых аминокислот [13]. Кроме того, ряд вителлогенинов в её составе способен ослаблять окислительный стресс путём поглощения свободных радикалов или посредством противомикробной и противовирусной активности [14,15].…”

Section: Introductionunclassified

The Sturgeon Ovarian Liquid and the Perch Roe Extract are Able to Enhance the Recovery of the Fibroblasts after their Stress-induced Premature Senescence

Mikhailova

Belyaeva

Kozlova

et al. 2018

BMCRM

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Ovarian liquid and fish roe are valuable sources of biologically active compounds. In order to study mechanisms of aging and also to search for biologically active compounds capable of inhibiting aging, we have modelled premature senescence in human embryonic fibroblasts by incubating of cells with Н2О2. Cell senescence was assessed by staining for β-galactosidase (SA-β-Gal) at pH 6.0; cell proliferation was further evaluated by the optical method. The dried ovarian liquid of the Siberian sturgeon and the extract of the perch roe were able to enhance recovery of the cells after induction of their premature senescence caused by oxidative stress. In contrast to the extract from perch roe and the extracts from fish muscle, dried ovarian fluid gave such an effect without the growth of proliferation.

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Atlantic salmon (Salmo salar L.) serum vitellogenin neutralises infectivity of infectious pancreatic necrosis virus (IPNV)

Cited by 74 publications

References 18 publications

Comparative transcriptome analysis of testes and ovaries for the discovery of novel genes from Amur sturgeon (Acipenser schrenckii)

Comparative transcriptome analysis of testes and ovaries for the discovery of novel genes from Amur sturgeon (Acipenser schrenckii)

Deconstructing honeybee vitellogenin: novel 40 kDa fragment assigned to its N terminus

The Sturgeon Ovarian Liquid and the Perch Roe Extract are Able to Enhance the Recovery of the Fibroblasts after their Stress-induced Premature Senescence

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