Atmospheric pressure cold plasma as an antifungal therapy

Sun, Peng; Sun, Yi; Wu, Haiyan; Zhu, Weidong; López, José L.; Liu, Wei; Zhang, Jue; Li, Ruoyu; Fang, Jing

doi:10.1063/1.3530434

Cited by 84 publications

(58 citation statements)

References 27 publications

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“…Morfill et al report the same 4 log reductions with a treatment of only 5 s for a "barrier corona discharge" [115]. Notably, the inactivation of different Candida strains (although not biofilms) with a plasma jet was reported recently by Sun et al [80]. The device and treatment protocol was also used by Feng et al [79] and is based on the same design used in our study [49].…”

Section: Introductionsupporting

confidence: 60%

Cold DC-Operated Air Plasma Jet for the Inactivation of Infectious Microorganisms

Kolb

Mattson

Edelblute

et al. 2012

IEEE Trans. Plasma Sci.

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We evaluated a nonthermal plasma jet for a respective use to prevent infections from bacteria and yeasts. The plasma jet is generated from the flow of ambient air with 8 slm through a microhollow cathode discharge assembly that is operated with a direct current of 30 mA. With these parameters, the temperature in the jet reaches 43 • C at 10 mm from the discharge. Agar plates that were inoculated with Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumannii, and Candida kefyr were treated at this distance, moving the plates through the jet in a meander that covered a 2 cm by 2 cm area. Different exposure times were realized by changing the speed of the movement and adjusting the distance between consecutive passes. S. aureus was most responsive to the exposure with a reduction in the number of colony forming units of 5.5 log steps in 40 s. All other microorganisms show a more gradual inactivation with exposure times. For all bacteria, a clearing of the treated area is achieved in about 2.5-3.5 min, corresponding to log-reduction factors of 5.5-6.5. Complete inactivation of the yeast requires about 7 min. Both S. aureus and C. kefyr show considerable inactivation also outside the immediate treatment area, while P. aeruginosa and A. baumannii do not. We conclude that differences in the morphologies of the membrane structures are responsible for the diverging results, together with a targeted response to different agents provided with the plasma jet. For the gram negative bacteria, we hold short-lived agents, acting across a short range, responsible, while for the other microorganisms, longer lived species seem more important. Our measurements show that neither heat, ultraviolet radiation, nor the generation of ozone can be responsible for the observed results. The most prominent long lived reaction product found is nitric oxide, which, by itself or through induced chemical reactions, might affect cell viability.

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Section: Introductionsupporting

confidence: 60%

Cold DC-Operated Air Plasma Jet for the Inactivation of Infectious Microorganisms

Kolb

Mattson

Edelblute

et al. 2012

IEEE Trans. Plasma Sci.

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“…Although a few studies have reported the successful treatment of dental microorganism in root canal cavity [8,12], the fungi biofilms are still a big challenge in clinical therapy. In our previous study, successful treatment 10 strains of Candida biofilms (4 strains of Candida albicans, 3 strains of Candida glabrata, and 3 strains of Candida krusei) in vitro were demonstrated within 1 min's treatment [25,28]. However, it is important to realize the complex of the environment can compromise the efficiency of the therapy [29].…”

Section: Discussionmentioning

confidence: 94%

“…Tim Maisch et al [27] found that plasma at room temperature can lead to an 3 * 5 log 10 reduction on 24-h Candida albicans biofilms formed on 6-well plates, which is comparable to 70 % ethanol treatment. Successful treatment of Candida albicans by cold He/O 2 plasma has been demonstrated both in petri dish and liquid environment [25,28]. However, it is crucial to understand the complexity of the biofilm microenvironment on PMMA may compromise the efficiency of the therapy [29].…”

Section: Introductionmentioning

confidence: 99%

Inactivation of Candida albicans Biofilms on Polymethyl Methacrylate and Enhancement of the Drug Susceptibility by Cold Ar/O2 Plasma Jet

Wang

Sun

Pan

et al. 2015

Plasma Chem Plasma Process

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The antimicrobial effects of the cold plasma for the dental pathogenic microorganism propose a promising approach to the Denture Stomatitis (DS) treatment. However, it is crucial to understand that the complexity of the biofilm microenvironment may compromise the efficiency of the therapy. As one of the major issue for DS, Candida albicans biofilms (ATCC10231) formed on denture base resins were treated by cold Ar/O 2 (2 %) plasma jet. Spatial viability of the biofilms was investigated with confocal scanning laser microscopy through evaluating their inside cross-section properties. Results showed Candida albicans biofilms with thickness of *100 lm was completely inactivated by 8 min plasma treatment. Morphology change of the fungi was also observed by the scanning electron microscopy. Drug susceptibilities, the sessile minimum inhibitory concentration (SMIC50) of the biofilm for amphotericin B and fluconazole were decreased from [32 and [256 lg/mL to 8 and 64 lg/mL after 1 min's plasma treatment, respectively. The reactive species produced from plasma were monitored by optical emission spectroscopy. The successfully inactivation of Candida albicans biofilms and the significant enhancement of its drug susceptibilities induced by the plasma released reactive species propose a promising strategy for the treatment of DS caused by drug-resistant Candida albicans biofilms.

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“…36.9 ) [1][2][3]16 ] . The effects of electromagnetic fi elds on biological objects are still insuffi ciently studied.…”

Section: Fungicidal Effect Of Plasma and Radio-wave Treatmentmentioning

confidence: 98%

Fungicidal Effects of Plasma and Radio-Wave Pre-treatments on Seeds of Grain Crops and Legumes

Филатова

Ажаронок

Shik³

et al. 2011

NATO Science for Peace and Security Series A: Chemistry and Biology

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Atmospheric pressure cold plasma as an antifungal therapy

Cited by 84 publications

References 27 publications

Cold DC-Operated Air Plasma Jet for the Inactivation of Infectious Microorganisms

Cold DC-Operated Air Plasma Jet for the Inactivation of Infectious Microorganisms

Inactivation of Candida albicans Biofilms on Polymethyl Methacrylate and Enhancement of the Drug Susceptibility by Cold Ar/O2 Plasma Jet

Fungicidal Effects of Plasma and Radio-Wave Pre-treatments on Seeds of Grain Crops and Legumes

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