Atomic force microscopy measurement of the elastic properties of the kidney epithelial cells

Adhesion force between calcium oxalate monohydrate crystal and kidney epithelial cells and possible relevance for kidney stone formation

“…In distinction from Ref. [5], where the force was measured between a silicon nitride tip and the biological cell, in the present paper the force was measured between a cell and the COM crystal, mounted by thermal epoxy glue onto the tip, as it is shown in Fig. 1.…”

“…The growth and acclimatization of LLC-PK 1 (ATCC, Gaithersburg, MD, Cat # CL-101) and MDCK (ATCC, Gaithersburg, MD, Cat # CCR-34) cells is described in Ref. [5].…”

“…This study was made because the hyperoxaluria (the excess of the oxalate concentration) is one of the dangerous kidney diseases. Before exposure to Ox, the acclimatization media [5] was removed and fresh acclimatization media was added with or without Ox.…”

“…[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15]). Some of these papers have dealt with renal epithelial cells, such as MDCK and LLC-PK 1 [4,5]. The properties of these cells were investigated in connection with the problem of forming of the kidney stones.…”

“…In our previous paper [5] the interaction of an AFM tip with kidney epithelial cells (MDCK and LLC-PK 1 ) was measured.…”