Atomic force microscopy-mediated mechanobiological profiling of complex human tissues

Cho, David H.; Aguayo, Sebastian; Cartagena-Rivera, Alexander X.

doi:10.1016/j.biomaterials.2023.122389

Cited by 11 publications

(2 citation statements)

References 171 publications

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“…However, it is important to apply the required corrections for the processing, such as the correction for the finite sample thickness. Finally, AFM is a standard technique for the measurement of mechanical properties at the nano- and microscale, which means certain specificity related to this spatial scale, surface effects, and local heterogeneities (Cho et al, 2023; Efremov et al, 2020b). On the other hand, AFM provides the data at the scale where cells interact with the surrounding media, which is important in the development and testing of biomaterials.…”

Section: Resultsmentioning

confidence: 99%

Mechanical characterization of soft biomaterials: which time and spatial scale to choose?

Krivega,

Kotova,

Timashev

et al. 2024

Preprint

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The mechanical properties of soft gels hold significant relevance in biomedicine and biomaterial design, including the development of tissue engineering constructs and bioequivalents. It is important to adequately characterize the gel's mechanical properties since they play a role both in the overall structural properties of the construct and in cells' physiological responses. The question remains which approach for mechanical characterization is most suitable for specific biomaterials. Our investigation is centered on the comparison of three types of gels and four distinct mechanical testing techniques: shear rheology, compression, microindentation, and nanoindentation by atomic force microscopy. While analyzing an elastic homogeneous synthetic hydrogel (polyacrylamide gel), we observed close mechanical results across the different testing techniques. However, our findings revealed more distinct outcomes when assessing a highly viscoelastic gel (Ecoflex) and a heterogeneous biopolymer hydrogel (enzymatically crosslinked gelatin). To ensure the precise data interpretation, we introduced correction factors to account for the boundary conditions inherent in many of the testing methods. The results of the study underscore the critical significance of considering both the temporal and spatial scales in mechanical measurements of biomaterials. Furthermore, they encourage the employment of a combination of diverse testing techniques, particularly in the characterization of heterogeneous viscoelastic materials such as biological samples. The obtained results will contribute to refining mechanical testing protocols and advance the development of soft gels for tissue engineering.

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Section: Resultsmentioning

confidence: 99%

Mechanical characterization of soft biomaterials: which time and spatial scale to choose?

Krivega,

Kotova,

Timashev

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Finally, AFM is a standard technique for the measurement of mechanical properties at the nano- and microscale, which means certain specificity related to this spatial scale, surface effects, and local heterogeneities. 62,63 The method requires careful calibration of the cantilever stiffness, tip radius, and precise adjustment of the optical lever system to obtain reliable mechanical values, as emphasized in the recent works. 36,64 On the other hand, AFM provides the data at the scale where cells interact with the surrounding media, which is important in the development and testing of biomaterials.…”

Section: Resultsmentioning

confidence: 99%

Mechanical characterization of soft biomaterials: which time and spatial scale to choose?

Krivega,

Kotova,

Timashev

et al. 2024

Soft Matter

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Pathogenesis‐Guided Engineering: pH‐Responsive Imprinted Polymer Co‐Delivering Folate for Inflammation‐Resolving as Immunotherapy in Implant‐Related Infections

Costa,

Nagay,

Villa

et al. 2024

Adv Funct Materials

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Folate (FT) is a suitable targeting ligand for folate receptors (FOLR) overexpressed on inflamed cells. Thus, FT‐loaded polymers can be used as FOLRs‐targeted immunotherapy to positively modulate the inflammatory process. A novel biodegradable imprinted polymer with a FT delivery mechanism driven by pH changes [PCL‐MIP@FT] is designed with molecularly imprinted technology. The pH mechanism is validated in vitro, demonstrating that an acidic environment accelerated and increased the release of FT for a period of 7 days (∼100 µg mL−1). For the first time, FT receptors (FOLR‐1 and FOLR‐3) are discovered and also overexpressed on activated human gingival fibroblasts, representing a favorable target in the oral environment. Although FT itself does not have antimicrobial effects, the nanomechanical properties of biofilm are changed after topical FT administration. In vivo systemic toxicity of PCL‐MIP@FT has been demonstrated to be a safe biomaterial (up to 1.3 mg kg−1). When the PCL‐MIP@FT is assessed in the subcutaneous tissue, it promoted an alleviating inflammation and may be able to stimulate tissue repair. The present findings have demonstrated the reliable in vitro and in vivo anti‐inflammatory actions of FT‐loaded polymer and support its use as a novel drug‐free therapeutic platform for modulating and mitigating inflammatory responses in dental implant‐related infections.

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Atomic force microscopy-mediated mechanobiological profiling of complex human tissues

Cited by 11 publications

References 171 publications

Mechanical characterization of soft biomaterials: which time and spatial scale to choose?

Mechanical characterization of soft biomaterials: which time and spatial scale to choose?

Mechanical characterization of soft biomaterials: which time and spatial scale to choose?

Pathogenesis‐Guided Engineering: pH‐Responsive Imprinted Polymer Co‐Delivering Folate for Inflammation‐Resolving as Immunotherapy in Implant‐Related Infections

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