ATP binding cassette proteins ABCG37 and ABCG33 function as potassium-independent cesium uptake carriers in Arabidopsis roots

Ashraf, M. Arif; Akihiro, Takashi; Ito, Kenichi; Kumagai, Sayaka; Sugita, Ryohei; Tanoi, Keitaro; Rahman, Abidur

doi:10.1016/j.molp.2021.02.002

Cited by 23 publications

(13 citation statements)

References 57 publications

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“…Cold stress down regulates SEC7 containing ARF-GEF GNOM at translational level in Arabidopsis [ 56 ]. Long-term (Cs + ) metal stress reduces expression of cesium uptake transporters ABCG37 and ABCG33 without affecting their transcription [ 57 ]. E3 ubiquitin ligase IRT Degradation Factor 1 (IDF1) degrades ferrous Fe uptake transporter IRON-REGULATED TRANSPORTER1 (IRT1) depending on environmental conditions to maintain Fe homeostasis [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

Actin Isovariant ACT7 Modulates Root Thermomorphogenesis by Altering Intracellular Auxin Homeostasis

Parveen

Rahman

2021

IJMS

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High temperature stress is one of the most threatening abiotic stresses for plants limiting the crop productivity world-wide. Altered developmental responses of plants to moderate-high temperature has been shown to be linked to the intracellular auxin homeostasis regulated by both auxin biosynthesis and transport. Trafficking of the auxin carrier proteins plays a major role in maintaining the cellular auxin homeostasis. The intracellular trafficking largely relies on the cytoskeletal component, actin, which provides track for vesicle movement. Different classes of actin and the isovariants function in regulating various stages of plant development. Although high temperature alters the intracellular trafficking, the role of actin in this process remains obscure. Using isovariant specific vegetative class actin mutants, here we demonstrate that ACTIN 7 (ACT7) isovariant plays an important role in regulating the moderate-high temperature response in Arabidopsis root. Loss of ACT7, but not ACT8 resulted in increased inhibition of root elongation under prolonged moderate-high temperature. Consistently, kinematic analysis revealed a drastic reduction in cell production rate and cell elongation in act7-4 mutant under high temperature. Quantification of actin dynamicity reveals that prolonged moderate-high temperature modulates bundling along with orientation and parallelness of filamentous actin in act7-4 mutant. The hypersensitive response of act7-4 mutant was found to be linked to the altered intracellular auxin distribution, resulted from the reduced abundance of PIN-FORMED PIN1 and PIN2 efflux carriers. Collectively, these results suggest that vegetative class actin isovariant, ACT7 modulates the long-term moderate-high temperature response in Arabidopsis root.

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Section: Discussionmentioning

confidence: 99%

Actin Isovariant ACT7 Modulates Root Thermomorphogenesis by Altering Intracellular Auxin Homeostasis

Parveen

Rahman

2021

IJMS

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“…ABCG33 and ABCG37, two ABC proteins, have been shown to mediate redundantly potassium-independent Cs + influx in Arabidopsis roots (Ashraf et al 2021). In contrast, transporters mediating Cs + translocation from the roots and the shoot remain unclear.…”

Section: Kup9 Mutants Accumulate More Cs +mentioning

confidence: 99%

Disruption of AtHAK/KT/KUP9 enhances plant cesium accumulation under low potassium supply

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Martin

Kanno

et al. 2021

Physiologia Plantarum

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Understanding the molecular mechanisms that underlie cesium (Cs + ) transport in plants is important to limit the entry of its radioisotopes from contaminated areas into the food chain. The potentially toxic element Cs + , which is not involved in any biological process, is chemically closed to the macronutrient potassium (K + ). Among the multiple K + carriers, the high-affinity K + transporters family HAK/KT/KUP is thought to be relevant in mediating opportunistic Cs + transport. Of the 13 KUP identified in A. thaliana, only HAK5, the major contributor to root K + acquisition under low K + supply, has been functionally demonstrated to be involved in Cs + uptake in planta. In the present study, we showed that accumulation of Cs + increased by up to 30% in two A. thaliana mutant lines lacking KUP9 and grown under low K + supply.Since further experiments revealed that Cs + release from contaminated plants to the external medium is proportionally lower in the two kup9 mutant alleles, we proposed that KUP9 disruption could impair Cs + efflux. By contrast, K + status in kup9 mutants is not affected, suggesting that KUP9 disruption does not alter substantially K + transport in experimental conditions used. The putative primary role of KUP9 in plants is further discussed. | INTRODUCTIONCesium is an alkali metal generally occurring at low concentrations in soil solution, predominantly as the monovalent cation Cs + (Greenwood & Earnshaw 1997). Although it has not been implicated in any biological process to date, Cs + is taken up by plants and can even be toxic (Hampton et al. 2004). Due to its low environmental concentration, the chemical toxicity of stable Cs + is rarely relevant in

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“…Arabidopsis DSO/ABCG11 transporter affects cutin and suberin Metabolism in reproductive organs and roots tissues [ 20 ]. Similarly, ABCG37 and ABCG33 functioned as caesium influx carriers in Arabidopsis roots [ 21 ]. These suggested the crucial roles of ABCG subfamily members to improve plant growth, development, and nutrients acquisition.…”

Section: Introductionmentioning

confidence: 99%

Identification of ABC transporter G subfamily in white lupin and functional characterization of L.albABGC29 in phosphorus use

et al. 2021

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Background White lupin (Lupinus albus) is a leguminous crop with elite adaptive ability in phosphorus-deficient soil and used as a model plant for studying phosphorus (P) use. However, the genetic basis of its adaptation to low P (LP) remains unclear. ATPase binding cassette (ABC) transports G subfamily play a crucial role in the transportation of biological molecules across the membrane. To date, identification of this subfamily has been analyzed in some plants, but no systematic analysis of these transporters in phosphorus acquisition is available for white lupin. Results This study identified 66 ABCG gene family members in the white lupin genome using comprehensive approaches. Phylogenetic analysis of white lupin ABCG transporters revealed six subclades based on their counterparts in Arabidopsis, displaying distinct gene structure and motif distribution in each cluster. Influences of the whole genome duplication on the evolution of L.albABCGs were investigated in detail. Segmental duplications appear to be the major driving force for the expansion of ABCGs in white lupin. Analysis of the Ka/Ks ratios indicated that the paralogs of the L.albABCG subfamily members principally underwent purifying selection. However, it was found that L.albABCG29 was a result of both tandem and segmental duplications. Overexpression of L.albABCG29 in white lupin hairy root enhanced P accumulation in cluster root under LP and improved plant growth. Histochemical GUS staining indicated that L.albABCG29 expression increased under LP in white lupin roots. Further, overexpression of L.albABCG29 in rice significantly improved P use under combined soil drying and LP by improving root growth associated with increased rhizosheath formation. Conclusion Through systematic and comprehensive genome-wide bioinformatics analysis, including conserved domain, gene structures, chromosomal distribution, phylogenetic relationships, and gene duplication analysis, the L.albABCG subfamily was identified in white lupin, and L.albABCG29 characterized in detail. In summary, our results provide deep insight into the characterization of the L.albABCG subfamily and the role of L.albABCG29 in improving P use.

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ATP binding cassette proteins ABCG37 and ABCG33 function as potassium-independent cesium uptake carriers in Arabidopsis roots

Cited by 23 publications

References 57 publications

Actin Isovariant ACT7 Modulates Root Thermomorphogenesis by Altering Intracellular Auxin Homeostasis

Actin Isovariant ACT7 Modulates Root Thermomorphogenesis by Altering Intracellular Auxin Homeostasis

Disruption of AtHAK/KT/KUP9 enhances plant cesium accumulation under low potassium supply

Identification of ABC transporter G subfamily in white lupin and functional characterization of L.albABGC29 in phosphorus use

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