Atractylodes macrocephala Koidz promotes intestinal epithelial restitution via the polyamine—Voltage-gated K+ channel pathway

Song, Haixing; Li, Ru-Liu; Xu, Chen; Wang, Yiyu; Cai, Jiazhong; Liu, Jia; Chen, Weiwen

doi:10.1016/j.jep.2013.12.049

Cited by 33 publications

(20 citation statements)

References 42 publications

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“…Modern studies have demonstrated that extracts or compounds from CR [7][8][9] or GRR [10][11][12][13] possess multiple physiological effects, such as anti-inflammation, antioxidation, gastrointestinal function regulation, and immune function regulation. We have shown that AMR stimulated gastrointestinal epithelial repair through polyamine-mediated Ca 2+ and K + signaling pathways [14][15][16]. In addition, Chrubasik et al reported the antiinflammatory activity and immune-modulatory effects of ZR [17,18].…”

Section: Introductionmentioning

confidence: 75%

RETRACTED: Antiulcerogenic Activity of Li‐Zhong Decoction on Duodenal Ulcers Induced by Indomethacin in Rats: Involvement of TLR‐2/MyD88 Signaling Pathway

Song

Zeng

Chen

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Background. Administration of nonsteroidal anti-inflammatory drugs (NSAIDs) often causes small intestinal ulcers in patients, but few effective drugs are currently available to manage such serious adverse events of NSAIDs. Li-Zhong decoction (LZD), a well-known traditional Chinese medicine (TCM) formula, is commonly prescribed for treatment of gastrointestinal diseases. The present study aimed to investigate the anti-ulcerogenic activity of LZD on indomethacin- (IND-) induced duodenal ulcer in rats. Mechanistic studies of action of LZD were focused on involvement of TLR-2/MyD88 signaling pathway. Methods. Fifty male Sprague-Dawley (SD) rats were randomly and evenly divided into five groups: normal control, ulcer control (IND, 25 mg/kg), IND + esomeprazole (ESO, 4.17 mg/kg), and IND + low and high doses of LZD (3.75 and 7.50 g/kg). Macroscopic and histopathological examinations were performed for evaluation of ulcer index (UI), curative index (CI), and microscopic score (MS). Levels of duodenal inflammatory biomarkers and cytoprotective mediators including interleukin-4 (IL-4), IL-10, tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2) were measured by ELISA. Expression levels of TLR-2 and MyD88 mRNA were assessed by qRT-PCR. The expression and distribution of TLR-2 and MyD88 proteins were analyzed by western blot and immunohistochemistry, respectively. Results. Gross and microscopic examinations of the IND-treated rats revealed severe duodenal hemorrhagic necrosis, inflammatory infiltration, villus destruction, and crypt abscess, while LZD-treated rats manifested these pathological events to a markedly lesser degree. LZD significantly decreased UI and MS, increased CI, preserved the integrity of the villus and crypt, and normalized the tissue architecture of the duodenum of rats. The elevated TNF-α levels in the IND-treated rats were markedly diminished in the LZD-treated rats, while lower levels of IL-4, IL-10, and PGE2 observed in IND-treated rats were significantly increased in LZD-treated rats. Interestingly, improvement of immune function in duodenal mucosa by reduction of mRNA and protein expression levels of TLR-2 and MyD88 was also observed in rats treated with LZD. Consistently, immunohistochemical analyses revealed a lower co-localization of TLR-2 and MyD88 proteins in the duodenal mucosa of LZD-treated rats as compared to the IND-induced rats. Conclusions. Our data demonstrate that LZD protects the duodenal mucosa from IND-caused lesions, which is at least partially attributable to the interaction of its potential cytoprotective and anti-inflammatory mechanisms together with enhancement of the mucosal immunity through TLR-2/MyD88 signaling pathway.

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Section: Introductionmentioning

confidence: 75%

RETRACTED: Antiulcerogenic Activity of Li‐Zhong Decoction on Duodenal Ulcers Induced by Indomethacin in Rats: Involvement of TLR‐2/MyD88 Signaling Pathway

Song

Zeng

Chen

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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show abstract

“… 2 – 7 In addition, there were several reports that AM promotes the intestinal healing process of inflammation such as intestinal epithelial restitution. 8 TH, commonly known as dandelions, are traditionally used for anti-inflammatory and diuretic effect. According to recent reports, 9 – 13 TH may be effective at liver cancer, breast cancer or uterine cancer and also had antioxidant activities by inhibiting of process of nitric oxide and prostaglandin E2.…”

Section: Introductionmentioning

confidence: 99%

Heme Oxygenase-1 Induction and Anti-inflammatory Actions ofAtractylodes macrocephalaandTaraxacum herbaExtracts Prevented Colitis and Was More Effective than Sulfasalazine in Preventing Relapse

et al. 2017

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Background/AimsIn inflammatory bowel disease (IBD), repeated bouts of remission and relapse occur in patients and can impose a risk of colitis-associated cancer. We hypothesized that plant extracts of Atractylodes macrocephala (AM) or Taraxacum herba (TH) may be better than sulfasalazine for treating this disease because these extracts can promote additional regeneration.MethodsMurine intestinal epithelial IEC-6 cells were pretreated with AM or TH before a lipopolysaccharide (LPS)-induced challenge. Acute colitis was induced with 7 days of dextran sulfate sodium (DSS) in male C57BL/6 mice, and extracts of AM and TH were administered for 2 weeks before DSS administration.ResultsIn vitro studies demonstrated that AM or TH treatment reduced LPS-induced COX-2 and tumor necrosis factor-α mRNA levels but increased heme oxygenase-1 (HO-1). Oral preadministration of AM and TH rescued mice from DSS-induced colitis by inhibiting inflammatory mediators via inactivated extracellular signal regulated kinase and repressed nuclear factor κB and signal transducer and activator of transcription 3, but the effect was weaker for sulfasalazine than that for the extracts. Anti-inflammatory activities occurred via the inhibition of macrophage and T lymphocyte infiltrations. Unlike sulfasalazine, which did not induce HO-1, TH extracts afforded significant HO-1 induction.ConclusionsBecause the AM or TH extracts were far superior in preventing DSS-induced colitis than sulfasalazine, AM or TH extracts can be considered natural agents that can prevent IBD relapse.

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“…Recent studies showed that HuR is one of the key regulators maintaining the intestinal homeostasis post-transcriptionally (Liu et al, 2016). GA increased HuR protein levels both in cytoplasm (Figure 3A,B) and nucleus (Figure 3C,D), exhibiting the same modulatory effect as spermidine (SPD) (Figure 3A–D), one of the polyamines that promotes the proliferation of IECs (Song et al, 2014). The depletion of polyamines by DFMO increased HuR levels in cytoplasm (Figure 3E,F) while decreasing HuR levels in nucleus (Figure 3G,H).…”

Section: Resultsmentioning

confidence: 90%

Glycyrrhetinic Acid Maintains Intestinal Homeostasis via HuR

et al. 2019

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Glycyrrhetinic acid (GA) is one of the main components of the traditional Chinese medicine of licorice, which can coordinate and promote the effects of other medicines in the traditional prescription. We found that GA could promote the proliferation, decrease the apoptotic rate, and attenuate DFMO-elicited growth arrest and delay in restitution after wounding in IEC-6 cells via HuR. GA failed to promote proliferation and to suppress apoptosis after silencing HuR by siRNA in IEC-6 cells. Furthermore, with the model of small intestinal organoids developed from intestinal crypt stem cells, we found that GA could increase HuR and its downstream ki67 levels to promote intestinal organoid development. In the in vivo assay, GA was shown to maintain the integrity of the intestinal epithelium under the circumstance of 48 h-fasting in rats via raising HuR and its downstream genes such as EGF, EGFR, and MEK. These results suggested that via HuR modulation, GA could promote intestinal epithelium homeostasis, and therefore contribute to the absorption of constituents from other medicines co-existing in the traditional prescription with licorice in the small intestine. Our results provide a new perspective for understanding the effect of licorice on enhancing the therapeutic effect of traditional prescriptions according to the traditional Chinese medicine theory.

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Atractylodes macrocephala Koidz promotes intestinal epithelial restitution via the polyamine—Voltage-gated K+ channel pathway

Cited by 33 publications

References 42 publications

RETRACTED: Antiulcerogenic Activity of Li‐Zhong Decoction on Duodenal Ulcers Induced by Indomethacin in Rats: Involvement of TLR‐2/MyD88 Signaling Pathway

RETRACTED: Antiulcerogenic Activity of Li‐Zhong Decoction on Duodenal Ulcers Induced by Indomethacin in Rats: Involvement of TLR‐2/MyD88 Signaling Pathway

Heme Oxygenase-1 Induction and Anti-inflammatory Actions ofAtractylodes macrocephalaandTaraxacum herbaExtracts Prevented Colitis and Was More Effective than Sulfasalazine in Preventing Relapse

Glycyrrhetinic Acid Maintains Intestinal Homeostasis via HuR

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